Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic

doi:10.1016/S1671-2927(00)8668

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (8): 1377-1383.DOI: 10.1016/S1671-2927(00)8668

Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic

GUAN Da-wei, MA Ming-chao, MA Zhong-yu, JIANG Xin, LI Li, CAO Feng-ming, SHEN De-long, CHEN Hui-jun, LI Jun

1.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R.China
2.Key Laboratory of Plant Nutrition and Nutrient Cycling, Ministry of Agriculture, Beijing 100081, P.R.China

收稿日期:2011-05-09 出版日期:2012-08-01 发布日期:2012-09-09
通讯作者: Correspondence LI Jun, Tel/Fax: +86-10-82106208, E-mail: jli@caas.ac.cn
作者简介:GUAN Da-wei, Tel/Fax: +86-10-82108675, E-mail: dwguan@yahoo.com.cn;
基金资助:
This work was supported by the National 863 Program of China (2010AA10A203), the Basic Scientific and Business Fund and Central Public Research Project, China (202-10) and the Special Fund for Establishment of Modern Agricultural R&D System, Ministry of Agriculture, China (nycytx-004).

Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic

GUAN Da-wei, MA Ming-chao, MA Zhong-yu, JIANG Xin, LI Li, CAO Feng-ming, SHEN De-long, CHEN Hui-jun, LI Jun

1.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R.China
2.Key Laboratory of Plant Nutrition and Nutrient Cycling, Ministry of Agriculture, Beijing 100081, P.R.China

Received:2011-05-09 Online:2012-08-01 Published:2012-09-09
Contact: Correspondence LI Jun, Tel/Fax: +86-10-82106208, E-mail: jli@caas.ac.cn
About author:GUAN Da-wei, Tel/Fax: +86-10-82108675, E-mail: dwguan@yahoo.com.cn;
Supported by:
This work was supported by the National 863 Program of China (2010AA10A203), the Basic Scientific and Business Fund and Central Public Research Project, China (202-10) and the Special Fund for Establishment of Modern Agricultural R&D System, Ministry of Agriculture, China (nycytx-004).

摘要/Abstract

摘要： The symbiotic matching for nodulation of Bradyrhizobium japonicum strains is a synergy of multi-proteins and plays a key role in symbiotic nitrogen fixation in nature. Studies on mechanism of symbiotic matching are significant in both theory and practice. In this paper, B. japonicum USDA110-A with high symbiotic matching with high-oil content soybean cultivar Suinong 20 and B. japonicum 2178 with low symbiotic matching were selected for proteomic to reveal mechanism of different symbiotic nodulation. The results showed that the amount and categories of proteins identified in this test were different when the two strains were treated by symbiotic nodulation. There were 10 up-regulated proteins and 5 down-regulated proteins with significant difference for B. japonicum USDA110-A. Proteins associated with nodulation and metabolism of energy and material, which were propitious to symbiotic nodulation, were all up-regulated, such as PHDPS synthase, metal-dependent phosphohydrolase, glycosyl transferase family. In contrast, only 5 up-regulated and 7 down-regulated differential proteins were detected in B. japonicum 2178. Molecular chaperones and defensive proteins, which influence the folding of nascent polypeptide chains and the active of azotase were down-regulated. To a certain extent, the different responses of B. japonicum to daidzein were one of the most important reasons that cause varieties in symbiotic matching ability.

关键词: Bradyrhizobium japonicum, daidzein, symbiotic matching for nodulation, proteomic technique

Abstract: The symbiotic matching for nodulation of Bradyrhizobium japonicum strains is a synergy of multi-proteins and plays a key role in symbiotic nitrogen fixation in nature. Studies on mechanism of symbiotic matching are significant in both theory and practice. In this paper, B. japonicum USDA110-A with high symbiotic matching with high-oil content soybean cultivar Suinong 20 and B. japonicum 2178 with low symbiotic matching were selected for proteomic to reveal mechanism of different symbiotic nodulation. The results showed that the amount and categories of proteins identified in this test were different when the two strains were treated by symbiotic nodulation. There were 10 up-regulated proteins and 5 down-regulated proteins with significant difference for B. japonicum USDA110-A. Proteins associated with nodulation and metabolism of energy and material, which were propitious to symbiotic nodulation, were all up-regulated, such as PHDPS synthase, metal-dependent phosphohydrolase, glycosyl transferase family. In contrast, only 5 up-regulated and 7 down-regulated differential proteins were detected in B. japonicum 2178. Molecular chaperones and defensive proteins, which influence the folding of nascent polypeptide chains and the active of azotase were down-regulated. To a certain extent, the different responses of B. japonicum to daidzein were one of the most important reasons that cause varieties in symbiotic matching ability.

Key words: Bradyrhizobium japonicum, daidzein, symbiotic matching for nodulation, proteomic technique

GUAN Da-wei, MA Ming-chao, MA Zhong-yu, JIANG Xin, LI Li, CAO Feng-ming, SHEN De-long, CHEN Hui-jun, LI Jun. Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic[J]. Journal of Integrative Agriculture, 2012, 12(8): 1377-1383.

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Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic

Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic

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