Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (6): 827-837.DOI: 10.1016/S1671-2927(11)60068-0

• 论文 • 上一篇    下一篇

Natural Variation of Pto and Fen Genes and Marker-Assisted Selection for Resistance to Bacterial Speck in Tomato

SUN Wan-yu, ZHAO Wan-ying, WANG Yuan-yuan, PEI Cheng-cheng and YANG Wen-cai   

  1. Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2011-06-10 修回日期:2011-06-10 出版日期:2011-06-20 发布日期:2011-06-03
  • 通讯作者: YANG Wen-cai
  • 作者简介:SUN Wan-yu, E-mail: wanyu-920@163.com; Correspondence YANG Wen-cai, Professor, Tel: +86-10-62734136, E-mail: yangwencai@cau.edu.cn

Natural Variation of Pto and Fen Genes and Marker-Assisted Selection for Resistance to Bacterial Speck in Tomato

SUN Wan-yu, ZHAO Wan-ying, WANG Yuan-yuan, PEI Cheng-cheng and YANG Wen-cai   

  1. Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
  • Received:2011-06-10 Revised:2011-06-10 Online:2011-06-20 Published:2011-06-03
  • Contact: YANG Wen-cai
  • About author:SUN Wan-yu, E-mail: wanyu-920@163.com; Correspondence YANG Wen-cai, Professor, Tel: +86-10-62734136, E-mail: yangwencai@cau.edu.cn

摘要: The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or AvrPtoB. Fen is a gene encoding closely related functional protein kinases as the Pto gene. To investigate the status of resistance to the pathogen and natural variation of Pto and Fen genes in tomato, 67 lines including 29 growing in China were subject to disease resistance evaluation and fenthion-sensitivity test. Alleles of Pto and Fen were amplified from genomic DNA of 25 tomato lines using polymerase chain reaction (PCR) and sequences were determined by sequencing the PCR products. The results indicated that none of the 29 cultivars/hybrids growing in China were resistant to bacterial speck race 0 strain DC3000. Seven of eight tomato lines resistant to DC3000 were also fenthion-sensitive. Analysis of deduced amino acid sequences identified three novel residue substitutions between Pto and pto, and one new substitution identified between Fen and fen. A PCR-based marker was developed and successfully used to select plants with resistance to DC3000.

关键词: tomato, bacterial speck, Pto, Fen, natural variation, marker-assisted selection

Abstract: The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or AvrPtoB. Fen is a gene encoding closely related functional protein kinases as the Pto gene. To investigate the status of resistance to the pathogen and natural variation of Pto and Fen genes in tomato, 67 lines including 29 growing in China were subject to disease resistance evaluation and fenthion-sensitivity test. Alleles of Pto and Fen were amplified from genomic DNA of 25 tomato lines using polymerase chain reaction (PCR) and sequences were determined by sequencing the PCR products. The results indicated that none of the 29 cultivars/hybrids growing in China were resistant to bacterial speck race 0 strain DC3000. Seven of eight tomato lines resistant to DC3000 were also fenthion-sensitive. Analysis of deduced amino acid sequences identified three novel residue substitutions between Pto and pto, and one new substitution identified between Fen and fen. A PCR-based marker was developed and successfully used to select plants with resistance to DC3000.

Key words: tomato, bacterial speck, Pto, Fen, natural variation, marker-assisted selection