CmERFV-2调节CmCBF3和CmMYB44抑制低温下甜瓜果实蔗糖的积累

doi:10.1016/j.jia.2025.12.022

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (10): 4223-4235.DOI: 10.1016/j.jia.2025.12.022

CmERFV-2调节CmCBF3和CmMYB44抑制低温下甜瓜果实蔗糖的积累

收稿日期:2025-06-05 修回日期:2025-12-11 接受日期:2025-10-18 出版日期:2026-10-20 发布日期:2026-09-21

CmERFV-2 regulates CmCBF3 and CmMYB44 to inhibit sucrose accumulation in oriental melon fruit at low temperature

Fan Yang^{1, 2}, Ge Gao^{1, 2}, Cheng Wang^{1, 2}, Jingyue Guan^{1, 2}, Hongyan Qi^{1, 2}^#

¹College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
²Key Laboratory of Protected Horticulture of Education of Ministry and Liaoning Province/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China

Received:2025-06-05 Revised:2025-12-11 Accepted:2025-10-18 Online:2026-10-20 Published:2026-09-21
About author:Fan Yang, E-mail: yf2021200103@163.com; #Correspondence Hongyan Qi, E-mail: qihongyan@syau.edu.cn, hyqiaaa@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (U20A2044), the China Agriculture Research System of MOF and MARA (CARS-25) and Scientific and Technology Program, Liaoning, China (2022JH1/10200004).

摘要/Abstract

摘要：

低温可以参与调节植物生长、发育和品质形成。然而，低温影响甜瓜果实蔗糖积累的机制目前尚不清楚。在此，以‘HS’（High Sucrose）甜瓜为研究材料，在乙烯即将释放的阶段，分别在30/18 ℃（昼/夜）和22/10 ℃（昼/夜）的温度下处理进行。低温显著抑制了甜瓜果实的乙烯释放和蔗糖积累，而在低温下进行乙烯利处理部分恢复了甜瓜果实的乙烯产量和蔗糖含量。通过酵母单杂交,GUS活性分析以及荧光素酶检测，我们发现转录因子CmCBF3可以结合在CmACO1（ACC氧化酶1）的启动子上并抑制其活性，从而抑制乙烯的产生。在低温下过表达CmCBF3显著抑制了乙烯和蔗糖的合成。进一步的研究表明，低温可以促进CmERFV-2的表达，而CmERFV-2可以与CmCBF3启动子结合，进一步抑制乙烯合成。此外，CmMYB44作为一种负调控果实乙烯产生和蔗糖积累的转录因子，可以抑制CmACO1和CmSPS1（蔗糖磷酸合酶1）的表达。CmERFV-2通过与CmMYB44启动子结合进一步影响CmACO1和CmSPS1的表达，从而在低温下调节乙烯释放量和蔗糖含量。综上所述，本研究揭示了CmERFV-2影响甜瓜果实乙烯释放和蔗糖积累的作用机制，为低温环境下培育优质甜瓜品种奠定了基础。

Abstract:

Low temperature is involved in regulating plant growth, development, and quality formation. The mechanism by which low temperature affects sucrose accumulation in oriental melon fruit is currently unclear. Here, high-sucrose (HS) melons were used as the research materials and subjected to two temperature regimes of 30°C/18°C (day/night) and 22°C/10°C (day/night) at the onset of ethylene production. Low temperature significantly inhibited ethylene release and sucrose accumulation in melon fruit, while ethephon treatment at low temperature partially restored the ethylene production and sucrose content. Using yeast one-hybrid (Y1H), GUS activity analysis, and luciferase assay, we found that the transcription factor CmCBF3 could bind to CmACO1 (ACC oxidase 1) promoter and inhibit its activity, thereby suppressing ethylene production. Overexpression of CmCBF3 under low temperature significantly inhibited the synthesis of ethylene and sucrose. Further research showed that low temperature promoted CmERFV-2 expression, and CmERFV-2 could bind to CmCBF3 promoter to further inhibit ethylene synthesis. In addition, CmMYB44, a transcription factor that negatively regulated fruit ethylene production and sucrose accumulation, repressed the expression of CmACO1 and CmSPS1 (sucrose phosphate synthase 1). CmERFV-2 further affected the expression of CmACO1 and CmSPS1 by binding to CmMYB44 promoter, thereby regulating ethylene and sucrose content at low temperature. In summary, this study revealed the mechanism by which CmERFV-2 affects ethylene release and sucrose accumulation in oriental melon fruit, laying a foundation for breeding high-quality melon varieties suitable for low-temperature cultivation.

Key words: low temperature , ethylene , sucrose , CmACO1 , CmSPS1 , CmERFV-2

Fan Yang, Ge Gao, Cheng Wang, Jingyue Guan, Hongyan Qi. CmERFV-2调节CmCBF3和CmMYB44抑制低温下甜瓜果实蔗糖的积累[J]. Journal of Integrative Agriculture, 2026, 25(10): 4223-4235.

Fan Yang, Ge Gao, Cheng Wang, Jingyue Guan, Hongyan Qi. CmERFV-2 regulates CmCBF3 and CmMYB44 to inhibit sucrose accumulation in oriental melon fruit at low temperature[J]. Journal of Integrative Agriculture, 2026, 25(10): 4223-4235.

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CmERFV-2调节CmCBF3和CmMYB44抑制低温下甜瓜果实蔗糖的积累

CmERFV-2 regulates CmCBF3 and CmMYB44 to inhibit sucrose accumulation in oriental melon fruit at low temperature

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