大豆萌发期耐盐负调控基因GmMACPF1的鉴定与功能研究

doi:10.1016/j.jia.2025.10.002

摘要/Abstract

摘要：

大豆是重要的粮油作物，土壤盐渍化是影响大豆生长发育的重要非生物胁迫因素之一。萌发期是大豆发育的关键阶段。本研究对165份大豆突变株系的萌发期耐盐性进行了评价，筛选出5份优异耐盐种质资源。全基因组关联分析（GWAS）鉴定出11个显著关联和44个潜在关联SNP位点，发现5个与耐盐性相关的新位点。对候选区域qtl5-1和qtl5-2分析，确定Glyma.05G097200和Glyma.05G240200(GmMACPF1)为候选基因，两个基因在耐盐和盐敏感基因型间表达模式不同。在拟南芥中过表达GmMACPF1降低耐盐性，而拟南芥macpf1突变体的耐盐性提高。GmMACPF1在大豆驯化过程中受到了选择，其单倍型Hap1和Hap3的大豆具有更强的耐盐性。以上结果表明GmMACPF1在萌发期负调控耐盐性，为解析大豆萌发期响应盐胁迫的分子机制提供了理论依据。

Abstract:

Soybeans, a crucial grain and oil crop, are valued for their high protein and oil content. Soil salinization is a significant abiotic stress that negatively impacts soybean growth and development, resulting in reduced yield and quality. The germination period represents a critical phase in soybean development. This study evaluated salt tolerance in 165 soybean mutant lines during germination, identifying five elite salt-tolerant germplasm resources. Multi-environment genome-wide association studies (GWASs) identified 11 significantly associated and 44 suggestive single nucleotide polymorphisms, alongside five novel quantitative trait loci linked to salt tolerance. Analysis of candidate regions qtl5-1 and qtl5-2 identified Glyma.05G097200 and Glyma.05G240200 as promising candidate genes, exhibiting distinct expression patterns between salt-tolerant and -sensitive genotypes. Functional characterization in Arabidopsis demonstrated that overexpression of the soybean gene GmMACPF1 induced salt sensitivity, while the macpf1 mutant of Arabidopsis displayed enhanced salt tolerance. Additionally, GmMACPF1 underwent selection during soybean domestication, with haplotypes Hap1 and Hap3 conferring improved salt tolerance. These results indicate that GmMACPF1 functions as a negative regulator of salt tolerance during germination, offering novel insights into the molecular mechanisms governing soybean response to salt stress during this crucial developmental stage.

Key words: soybean , salt stress , GWAS , GmMACPF1 , haplotype analysis

Zhiri Xu, Yajun Zhao, Xiaoting Zhang, Jie Huang, Jie Hu, Yuanpeng Liu, Deyue Yu, Guizhen Kan. 大豆萌发期耐盐负调控基因GmMACPF1的鉴定与功能研究[J]. Journal of Integrative Agriculture, 2026, 25(10): 4040-4053.

Zhiri Xu, Yajun Zhao, Xiaoting Zhang, Jie Huang, Jie Hu, Yuanpeng Liu, Deyue Yu, Guizhen Kan. Identification and functional characterization of GmMACPF1 as a negative regulator of salt tolerance during germination[J]. Journal of Integrative Agriculture, 2026, 25(10): 4040-4053.

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