芥菜型油菜高密度遗传图谱构建及芥酸，油酸和亚麻酸遗传调控解析

doi:10.1016/j.jia.2024.11.028

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2171-2189.DOI: 10.1016/j.jia.2024.11.028

芥菜型油菜高密度遗传图谱构建及芥酸，油酸和亚麻酸遗传调控解析

收稿日期:2024-01-28 修回日期:2024-11-12 接受日期:2024-06-11 出版日期:2025-06-20 发布日期:2025-05-12

Construction of a high-density genetic map to explore the genetic regulation of erucic acid, oleic acid, and linolenic acid contents in Brassica juncea

Wei Yan^1*, Jinze Zhang^1*, Yingfen Jiang², Kunjiang Yu¹, Qian Wang¹, Xu Yang¹, Lijing Xiao¹, Entang Tian^1#

¹Agricultural College, Guizhou University, Guiyang 550025, China
²Institute of Crop Science, Anhui Academy of Agricultural Sciences, Hefei 230001, China

Received:2024-01-28 Revised:2024-11-12 Accepted:2024-06-11 Online:2025-06-20 Published:2025-05-12
About author:#Correspondence Entang Tian, E-mail: erictian121@163.com *These authors contributed equally to this study.
Supported by:
This work was funded by the Scientific and Techno-logical Key Program of Guizhou Province, China (Qiankehezhicheng [2022] Key 031), the National Natural Science Foundation of China (32160483 and 32360497), the Post-Funded Project for the National Natural Science Foundation of China from Guizhou University ([2023]093), the Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province, China (Qiankehezhongyindi [2023]008), and the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions, China (Qianjiaoji [2023] 007).

摘要/Abstract

摘要：

芥菜型油菜是世界第三大油料作物，而其巨大表型变异背后的遗传机制大多未被深入研究。本文利用SLAF技术对来自芥菜型油菜Qichi881(蔬菜型)和YufengZC(油用型)亲本的197个F₈重组自交株系进行了重测序。序列分析共获得438895个高质量SLAF片段，包含47644个具有多态性片段及3887个可用于遗传图谱构建的多态性标记。创建的遗传图谱将3887个多态性标记分配到18个连锁群中,而获得的连锁群总长度为1830.23 cM, 相邻标记平均距离为0.47 cM。利用新创建的高密度遗传图谱，共检测到53个调控芥酸、油酸和亚麻酸含量的QTL，并将其整合成6个稳定表达的QTL，其中每个性状包含两个稳定表达QTL。对于每个性状来说，均有两个候选基因被克隆，而序列分析表明它们与各自稳定表达的QTL具有相同的位置。对于亚麻酸性状，开发出两个Bju.FAD3.A03和Bju.FAD3.B07基因的共显性标记，且均与亚麻酸含量表现出共分离现象，进一步证明了以上QTL定位及生物信息分析的准确性。此外，以上克隆基因的表达水平也进行了测定，并与作图群体不同株系的脂肪酸和含量表现出显著相关性。本研究结果将有助于芥菜型油菜脂肪酸性状的改良和分子育种。此外，本文还对新创建的高密度遗传图谱的潜在应用进行了讨论。

Abstract:

Rapeseed mustard (Brassica juncea L.) is the third most important oilseed crop in the world, but the genetic mechanism underlying its massive phenotypic variation remains largely unexplored. In this study, specific length amplified fragment sequencing (SLAF-Seq) was used to resequence a population comprising 197 F₈ recombinant inbred lines (RILs) derived from a cross between vegetable-type Qichi881 and oilseed-type YufengZC of B. juncea. In total, 438,895 high-quality SLAFs were discovered, 47,644 of which were polymorphic, and 3,887 of the polymorphic markers met the requirements for genetic map construction. The final map included 3,887 markers on 18 linkage groups and was 1,830.23 centiMorgan (cM) in length, with an average distance of 0.47 cM between adjacent markers. Using the newly constructed high-density genetic map, a total of 53 QTLs for erucic acid (EA), oleic acid (OA), and linolenic acid (LNA) were detected and integrated into eight consensus QTLs with two for each of these traits. For each of these three traits, two candidate genes were cloned and sequence analysis indicated colocalization with their respective consensus QTLs. The co-dominant allele-specific markers for Bju.FAD3.A03 and Bju.FAD3.B07 were developed and showed co-localization with their consensus QTLs and co-segregation with LNA content, further supporting the results of QTL mapping and bioinformatic analysis. The expression levels of the cloned homologous genes were also determined, and the genes were tightly correlated with the EA, OA and LNA contents of different lines. The results of this study will facilitate the improvement of fatty acid traits and molecular breeding of B. juncea. Further uses of the high-density genetic map created in this study are also discussed.

Key words: Brassica juncea , high-density genetic map , Bju.FAE1 , Bju.FAD2 , Bju.FAD3

Wei Yan, Jinze Zhang, Yingfen Jiang, Kunjiang Yu, Qian Wang, Xu Yang, Lijing Xiao, Entang Tian. 芥菜型油菜高密度遗传图谱构建及芥酸，油酸和亚麻酸遗传调控解析[J]. Journal of Integrative Agriculture, 2025, 24(6): 2171-2189.

Wei Yan, Jinze Zhang, Yingfen Jiang, Kunjiang Yu, Qian Wang, Xu Yang, Lijing Xiao, Entang Tian. Construction of a high-density genetic map to explore the genetic regulation of erucic acid, oleic acid, and linolenic acid contents in Brassica juncea[J]. Journal of Integrative Agriculture, 2025, 24(6): 2171-2189.

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芥菜型油菜高密度遗传图谱构建及芥酸，油酸和亚麻酸遗传调控解析

Construction of a high-density genetic map to explore the genetic regulation of erucic acid, oleic acid, and linolenic acid contents in Brassica juncea

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