水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生

doi:10.1016/j.jia.2024.08.013

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (4): 1566-1574.DOI: 10.1016/j.jia.2024.08.013

水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生

收稿日期:2024-02-20 修回日期:2024-08-22 接受日期:2024-07-12 出版日期:2026-04-20 发布日期:2026-03-11

A novel effector of Aphelenchoides besseyi, AbPFN3, interacts with multiple host proteins to facilitate parasitic nematode and sustain infection in rice

Xin Huang^{1, 3}, Yuankai Chi¹, Wei Zhao¹, Wenkun Huang², Deliang Peng^2#, Rende Qi^1#

¹Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China
²State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
³Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-02-20 Revised:2024-08-22 Accepted:2024-07-12 Online:2026-04-20 Published:2026-03-11
About author:Xin Huang, E-mail: huangxin0924@126.com; #Correspondence Deliang Peng, E-mail: dlpeng@ippcaas.cn; Rende Qi, E-mail: rende7@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (32001881) and the National Key Research and Development Program of China (2023YFD1400400).

摘要/Abstract

摘要：

水稻干尖线虫（Aphelenchoides besseyi）可侵染水稻、大豆、棉花等多种作物，给农业生产造成严重损失。目前水稻干尖线虫效应子的研究较少，该线虫的侵染分子机制仍不明确。研究水稻干尖线虫的致病分子机理，找到致病的关键靶标，对于水稻干尖线虫绿色防控技术的开发具有重要的意义。本研究使用原位杂交、qPCR、Co-IP/MS、BiFC、转基因过表达等技术对水稻干尖线虫分泌的一个关键致病效应子AbPFN3的功能进行了解析。AbPFN3基因全长381 bp，编码氨基酸长度126 aa。序列分析显示，AbPFN3与马铃薯白线虫的GpPFN3的序列相似性最高，为59.52%；AbPFN3具有典型的profilin蛋白结构域，但不具有信号肽和跨膜结构域。通过qPCR证实了AbPFN3在幼虫中高表达，使用原位杂交的方法得出AbPFN3是一个在食道腺特异表达的基因。亚细胞定位显示，AbPFN3被定位于内质网、叶绿体等多个细胞器上。使用Co-IP/MS、pull-down和BiFC等方法探究AbPFN3与寄主蛋白的互作，明确了水稻中与AbPFN3存在互作关系的蛋白有OsAAC1、OsBAP31和OsSAUR50，同时确定了这种互作发生在内质网、细胞质和质膜等多个细胞器中。在拟南芥中稳定表达AbPFN3基因后，植株的表型产生了明显变化，转基因拟南芥的株高显著高于野生型。在基因表达层面，转基因拟南芥中AAC1和BAP31基因的表达量显著上调，相反RGA2和SAUR50的表达量显著下调。综上所述，水稻干尖线虫AbPFN3是一个由食道腺分泌的效应子，与多个寄主蛋白存在互作关系，可能通过影响寄主的细胞发育、防卫反应、能量运输等过程促进水稻干尖线虫的侵染。本研究丰富了对水稻干尖线虫效应子分子互作模式的认识，证实了水稻干尖线虫的效应子可以通过与多个寄主蛋白的互作对寄主的多个生物学过程进行调控，进而促进线虫的侵染和寄生。

Abstract:

The rice white tip nematode (RWTN) Aphelenchoides besseyi secretes effectors that manipulate host plant cells to facilitate successful parasitism and sustain infection. Although the number of identified RWTN effectors remains limited, their mechanisms of interaction with host plants are largely unknown. Profilins (PFNs) function as molecular hubs that regulate complex interaction networks. To advance understanding of PFN3 in plant-parasitic nematodes, we identified an effector from A. besseyi, designated AbPFN3. AbPFN3 is transcriptionally upregulated during the juvenile stage of the nematode, and in situ hybridization localized its expression to the esophageal glands. Three rice (Oryza sativa) proteins, ADP/ATP carrier protein 1 (OsAAC1), B-cell receptor-associated protein 31 (OsBAP31) and Small Auxin Up RNA 50 (OsSAUR50), were identified as interactors of AbPFN3, with interactions occurring in distinct cellular compartments, including the endoplasmic reticulum, cytoplasm, and plasma membrane. Transgenic analyses revealed that AbPFN3 expression significantly increased plant height and upregulated AAC1 and BAP31, while downregulating RGA2 and SAUR50. This study characterizes AbPFN3 as a novel effector secreted by A. besseyi that interacts with multiple host proteins, highlighting its potential role in modulating host defense responses and cell development processes.

Key words: Aphelenchoides besseyi , profilin 3 , protein–protein interactions , effector

Xin Huang, Yuankai Chi, Wei Zhao, Wenkun Huang, Deliang Peng, Rende Qi. 水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生[J]. Journal of Integrative Agriculture, 2026, 25(4): 1566-1574.

Xin Huang, Yuankai Chi, Wei Zhao, Wenkun Huang, Deliang Peng, Rende Qi. A novel effector of Aphelenchoides besseyi, AbPFN3, interacts with multiple host proteins to facilitate parasitic nematode and sustain infection in rice[J]. Journal of Integrative Agriculture, 2026, 25(4): 1566-1574.

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水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生

A novel effector of Aphelenchoides besseyi, AbPFN3, interacts with multiple host proteins to facilitate parasitic nematode and sustain infection in rice

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