水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生

doi:10.1016/j.jia.2024.08.013

摘要/Abstract

摘要：

水稻干尖线虫（Aphelenchoides besseyi）可侵染水稻、大豆、棉花等多种作物，给农业生产造成严重损失。目前水稻干尖线虫效应子的研究较少，该线虫的侵染分子机制仍不明确。研究水稻干尖线虫的致病分子机理，找到致病的关键靶标，对于水稻干尖线虫绿色防控技术的开发具有重要的意义。本研究使用原位杂交、qPCR、Co-IP/MS、BiFC、转基因过表达等技术对水稻干尖线虫分泌的一个关键致病效应子AbPFN3的功能进行了解析。AbPFN3基因全长381 bp，编码氨基酸长度126 aa。序列分析显示，AbPFN3与马铃薯白线虫的GpPFN3的序列相似性最高，为59.52%；AbPFN3具有典型的profilin蛋白结构域，但不具有信号肽和跨膜结构域。通过qPCR证实了AbPFN3在幼虫中高表达，使用原位杂交的方法得出AbPFN3是一个在食道腺特异表达的基因。亚细胞定位显示，AbPFN3被定位于内质网、叶绿体等多个细胞器上。使用Co-IP/MS、pull-down和BiFC等方法探究AbPFN3与寄主蛋白的互作，明确了水稻中与AbPFN3存在互作关系的蛋白有OsAAC1、OsBAP31和OsSAUR50，同时确定了这种互作发生在内质网、细胞质和质膜等多个细胞器中。在拟南芥中稳定表达AbPFN3基因后，植株的表型产生了明显变化，转基因拟南芥的株高显著高于野生型。在基因表达层面，转基因拟南芥中AAC1和BAP31基因的表达量显著上调，相反RGA2和SAUR50的表达量显著下调。综上所述，水稻干尖线虫AbPFN3是一个由食道腺分泌的效应子，与多个寄主蛋白存在互作关系，可能通过影响寄主的细胞发育、防卫反应、能量运输等过程促进水稻干尖线虫的侵染。本研究丰富了对水稻干尖线虫效应子分子互作模式的认识，证实了水稻干尖线虫的效应子可以通过与多个寄主蛋白的互作对寄主的多个生物学过程进行调控，进而促进线虫的侵染和寄生。

Abstract:

The rice white tip nematode (RWTN) Aphelenchoides besseyi secretes effectors that manipulate the cells of its host plant and help the nematode to successfully parasitize and maintain infection in the host. The number of identified RWTN effectors is limited, and the mechanisms of RWTN effectors interacting with plants are largely unknown. Profilins (PFNs) function as hubs that control a complex network of molecular interactions. To gain full knowledge of PFN3 in plant parasitic nematodes, we identified an effector from A. besseyi named AbPFN3. AbPFN3 is transcriptionally upregulated in the juvenile stage of the nematode. In situ hybridization experiments showed that AbPFN3 transcribed in the nematode esophageal glands. Three AbPFN3-interacting proteins (OsAAC1, OsBAP31 and OsSAUR50) were found in the host plant, with interactions occurring in various locations such as the endoplasmic reticulum, cytoplasm, and plasma membrane. Transgenic analyses showed that the expression of AbPFN3 significantly increased plant height and upregulated the expression of AAC1 and BAP31 while downregulating RGA2 and SAUR50. This study describes a new effector protein, AbPFN3, secreted by A. besseyi, that interacts with multiple host proteins. These results suggest the important role of AbPFN3 in host defense response and cell development process.

Key words: Aphelenchoides besseyi , profilin 3 , , protein-protein interactions , , effector

. 水稻干尖线虫效应子AbPFN3通过与多个寄主蛋白的互作促进线虫的侵染与寄生[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.08.013.

Xin Huang, Yuankai Chi, Wei Zhao, Wenkun Huang, Deliang Peng, Rende Qi. A novel effector of Aphelenchoides besseyi, AbPFN3, interacts with multiple host proteins to assist parasitic nematode and maintain infection in rice[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.08.013.

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