Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (1): 56-67.DOI: 10.1016/j.jia.2024.07.006

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栽培花生AhPR10基因家族全基因组水平鉴定及在抵抗黄曲霉中的表达分析

  

  • 收稿日期:2024-03-11 修回日期:2024-07-04 接受日期:2024-05-21 出版日期:2026-01-20 发布日期:2025-12-05

Genome-wide characterization and expression analysis of the cultivated peanut AhPR10 gene family mediating resistance to Aspergillus flavus

Qi Zhao1, 2*, Mengjie Cui1, 3*, Tengda Guo1, Lei Shi1, 3, Feiyan Qi1, 3, Ziqi Sun1, 3, Pei Du1, 3, Hua Liu1, 3, Yu Zhang1, Zheng Zheng1, 3, Bingyan Huang1, 3, Wenzhao Dong1, 3, Suoyi Han1, 3#, Xinyou Zhang1, 3#   

  1. 1 Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences/Postgraduate T&R Base of Zhengzhou University/The Shennong Laboratory/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou 450002, China

    2 School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450066, China

    3  National Centre for Plant Breeding, Xinxiang 453500, China

  • Received:2024-03-11 Revised:2024-07-04 Accepted:2024-05-21 Online:2026-01-20 Published:2025-12-05
  • About author:#Correspondence Xinyou Zhang, E-mail: haasxinyou@163.com; Suoyi Han, E-mail: suoyi_han@126.com * These authors contributed equally to this study.
  • Supported by:
    This work was supported by the National Key R&D Program of China (2022YFD1200400) and the National Natural Science Foundation of China (32301851).

摘要:

病程相关蛋白PR10在植物生长、发育及胁迫响应中发挥重要作用。本研究对栽培花生AhPR10基因家族进行了全基因组水平的系统鉴定,并对其系统进化关系、保守基序、基因结构、共线性等进行分析。共鉴定出54个AhPR10基因,系统进化树将其分为八组,该结果已在基因结构和保守基序的特征分析中得到了证实。对其在染色体上的分布和共线性分析表明,片段复制事件是AhPR10基因家族扩展的关键。此外,AhPR10基因具有组成型和诱导型两种表达模式。值得注意的是,AhPR10-7AhPR10-33AhPR10-41基因可能在花生抵御黄曲霉菌的侵染过程中发挥关键作用。体外抑菌实验表明AhPR10-33融合蛋白能有效抑制黄曲霉菌丝生长。本研究为深入剖析和明确AhPR10在花生黄曲霉抗性中的作用奠定分子信息基础。

Abstract:

The pathogenesis-related protein PR10 plays a vital role in plant growth, development, and stress responses.  This study systematically identified and analyzed PR10 genes in cultivated peanut (Arachis hypogaea L.), examining their phylogenetic relationships, conserved motifs, gene structures, and syntenic relationships.  The analysis identified 54 AhPR10 genes, which were classified into eight groups based on phylogenetic relationships, supported by gene structure and conserved motif characterization.  Analysis of chromosomal distribution and synteny demonstrated that segmental duplications played a crucial role in the expansion of the AhPR10 gene family.  The identified AhPR10 genes exhibited both constitutive and inducible expression patterns.  Significantly, AhPR10-7, AhPR10-33, and AhPR10-41 demonstrated potential importance in peanut resistance to Aspergillus flavus.  In vitro fungistatic experiments demonstrated that recombinant AhPR10-33 effectively inhibited Aflavus mycelial growth.  These findings provide valuable insights for future investigations into AhPR10 functions in protecting peanut from Aflavus infection.


Key words: cultivated peanut , PR10 ,  phylogenetic analysis ,  expression pattern ,  Aspergillus flavus