全基因组分析揭示了花生AhCN34基因参与青枯病抗性研究

doi:10.1016/j.jia.2024.03.006

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 3757-3771.DOI: 10.1016/j.jia.2024.03.006

全基因组分析揭示了花生AhCN34基因参与青枯病抗性研究

收稿日期:2023-09-24 修回日期:2024-03-02 接受日期:2024-01-17 出版日期:2025-10-20 发布日期:2025-09-24

Genome-wide analysis of AhCN genes reveals that AhCN34 is involved in bacterial wilt resistance in peanut

College of Agronomy & Peanut Functional Genome and Molecular Breeding Engineering, Henan Agricultural University, Zhengzhou 450046, China

Received:2023-09-24 Revised:2024-03-02 Accepted:2024-01-17 Online:2025-10-20 Published:2025-09-24
About author:Kai Zhao, E-mail: zhaok816@163.com; #Correspondence Dongmei Yin, Tel: +86-371-56990188, E-mail: yindm@henau.edu.cn
Supported by:
This work was supported by the grants from the National Natural Science Foundation of China (NSFC)-Henan United Fund (U22A20475 and U1704232), the Key Scientific and Technological Project of Henan Province, China (221111110500, 161100111000, and HARS-22-05-G1), the Innovation Scientists and Technicians Troop Construction Projects of Henan Province, China (2018JR0001), the Henan Agricultural University High Level Talent Special Support Fund, China (30501418), and the Key Scientific Research Project in Colleges and Universities of Henan Province, China (21A210018).

摘要/Abstract

摘要：

花生（Arachis hypogaea L.）青枯病是一种严重威胁花生产量和品质的细菌性土传病害。核苷酸结合富亮氨酸重复序列（NBS-LRR）蛋白是一类植物特异性免疫受体，可识别病原体分泌的效应分子并激活免疫反应以抵抗病原体侵染。然而，在花生中AhCN基因（CN是一类缺乏LRR结构域的NLR基因）的确切功能尚不清楚。本研究通过系统进化发育分析，共鉴定出150个AhCN基因，划分为9个亚家族，发现AhCN基因表现出高度保守的结构特征，并参与植物激素信号转导和防御反应。接种青枯菌后，高抗性花生品种‘H108’在株高、主茎粗和鲜重等生理指标上显著高于感病品种‘H107’，这可能是由于青枯菌在维管束中的增殖和扩散受到抑制造成的。在遭受青枯菌侵染和植物激素处理后，q-PCR验证显示，与H107相比，AhCN34在H108中显著上调。重要的是，在叶片中过表达AhCN34增强了花生对青枯病的抗性。以上结果表明，AhCN34在花生抗病育种中具备一定的潜力，为今后花生高效遗传育种提供理论依据。

Abstract:

Peanut (Arachis hypogaea L.) bacterial wilt (BW) is a devastating soil-borne disease caused by Ralstonia solanacearum (RS) that poses a significant threat to peanut yield and quality. Nucleotide-binding leucine-rich repeat (NBS-LRR) proteins are a class of plant-specific immune receptors that recognize pathogen-secreted effector molecules and activate immune responses to resist pathogen infections. However, the precise functions of AhCN genes (where CN is a class of nucleotide-binding site, leucine-rich repeat receptor (NLR) genes that lack LRR structural domains) in peanut plants are not fully understood. In this study, a total of 150 AhCN genes were identified and classified into nine subfamilies based on a systematic phylogenetic analysis. The AhCN genes showed highly conserved structural features, and the promoter cis-elements indicated involvement in plant hormone signaling and defense responses. After inoculation with RS, the highly resistant peanut variety ‘H108’ significantly outperformed the susceptible variety ‘H107’ based on physiological indicators such as plant height, main stem diameter, and fresh weight, likely due to the inhibition of bacterial proliferation and diffusion in the stem vascular bundle. AhCN34 was found to be significantly upregulated in ‘H108’ compared to ‘H107’ during plant infection and in response to treatments with each of three plant hormones. Importantly, AhCN34 overexpression in peanut leaves enhanced their resistance to BW. These findings demonstrate the great potential of AhCN34 for applications in peanut resistance breeding. Our identification and characterization of the AhCN genes provide insights into the mechanisms underlying BW resistance in peanut and can inform future research into genetic methods of improving BW resistance in peanut.

Key words: peanut , bacterial wilt , resistance , NLR genes , disease

Kai Zhao, Yanzhe Li, Zhan Li, Zenghui Cao, Xingli Ma, Rui Ren, Kuopeng Wang, Lin Meng, Yang Yang, Miaomiao Yao, Yang Yang, Xiaoxuan Wang, Jinzhi Wang, Sasa Hu, Yaoyao Li, Qian Ma, Di Cao, Kunkun Zhao, Ding Qiu, Fangping Gong, Zhongfeng Li, Xingguo Zhang, Dongmei Yin. 全基因组分析揭示了花生AhCN34基因参与青枯病抗性研究[J]. Journal of Integrative Agriculture, 2025, 24(10): 3757-3771.

Kai Zhao, Yanzhe Li, Zhan Li, Zenghui Cao, Xingli Ma, Rui Ren, Kuopeng Wang, Lin Meng, Yang Yang, Miaomiao Yao, Yang Yang, Xiaoxuan Wang, Jinzhi Wang, Sasa Hu, Yaoyao Li, Qian Ma, Di Cao, Kunkun Zhao, Ding Qiu, Fangping Gong, Zhongfeng Li, Xingguo Zhang, Dongmei Yin. Genome-wide analysis of AhCN genes reveals that AhCN34 is involved in bacterial wilt resistance in peanut[J]. Journal of Integrative Agriculture, 2025, 24(10): 3757-3771.

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全基因组分析揭示了花生AhCN34基因参与青枯病抗性研究

Genome-wide analysis of AhCN genes reveals that AhCN34 is involved in bacterial wilt resistance in peanut

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