Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (1): 141-154.DOI: 10.1016/j.jia.2023.08.003

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  • 收稿日期:2023-03-10 接受日期:2023-07-12 出版日期:2024-01-20 发布日期:2024-01-06

Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17

Min Xu1, 2, Zhao Gao1, 2, Dalong Li1, Chen Zhang1, 2, Yuqi Zhang1, 2, Qian He1, 2, Yingbin Qi1, 2, He Zhang1, 2, Jingbin Jiang1, 2, Xiangyang Xu1, 2#, Tingting Zhao1, 2#   

  1. 1 College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China

    2 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, China

  • Received:2023-03-10 Accepted:2023-07-12 Online:2024-01-20 Published:2024-01-06
  • About author:#Correspondence Xiangyang Xu, E-mail: xxy709@126.com; Tingting Zhao, E-mail: ttzhao2016@163.com
  • Supported by:
    We thank the support from the National Natural Science Foundation of China (32102390 and 32072589), the China Agriculture Research System (CARS-23-A11), the Heilongjiang Provincial Natural Science Foundation of China (YQ2021C013), and the Northeast Agricultural University Scholars Program (20XG28), China.

Abstract:

PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress.  However, there has been very little research on the function of this family gene in tomatoes, which limits its application in germplasm resource improvement.  Therefore, the PLATZ gene family was identified and analyzed in tomato, and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function.  In this study, the PLATZ family members of tomato were identified in the whole genome, and 19 SlPLATZ genes were obtained.  Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis.  SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources.  SlPLATZ17 was selected for functional verification by experiment-based analysis.  The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants.  Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long, thin petals, adjacent petals were not connected at the base, and the stamen circumference was smaller.  This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.


Key words: tomato ,  PLATZ family ,  abiotic stresses , plant development