Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (9): 2719-2728.DOI: 10.1016/j.jia.2023.07.020

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基于SSR分子标记的安徽省茶树遗传多样性分析及核心种质构建

  

  • 收稿日期:2023-03-20 接受日期:2023-07-04 出版日期:2023-09-20 发布日期:2023-09-14

Core collection construction of tea plant germplasm in Anhui Province based on genetic diversity analysis using simple sequence repeat markers

TAO Ling-ling*, TING Yu-jie*, CHEN Hong-rong, WEN Hui-lin, XIE Hui, LUO Ling-yao, HUANG Ke-lin, ZHU Jun-yan, LIU Sheng-rui#, WEI Chao-ling#   

  1. State Key Laboratory of Tea Plant Biology and Utilization/Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture and Rural Affairs/Anhui Provincial Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, P.R.China
  • Received:2023-03-20 Accepted:2023-07-04 Online:2023-09-20 Published:2023-09-14
  • About author:TAO Ling-ling, E-mail: taoll0716@163.com; TING Yu-jie, E-mail: 18485364862@163.com; #Correspondence WEI Chao-ling, E-mail: weichl@ahau.edu.cn; LIU Sheng-rui, E-mail: liushengrui@ahau.edu.cn * These authors contributed equally to this study.
  • Supported by:
    This study was supported by the Project of Major Science and Technology of Anhui Province, China (202003a06020021), the National Key Research and Development Program of China (2021YFD1200200, 2021YFD1200203), the National Natural Science Foundation of China (U20A2045), the Base of Introducing Talents for Tea Plant Biology and Quality Chemistry (D20026), and the Anhui Provincial Natural Science Foundation, China (2108085QC121).

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摘要：

茶树是中国主要经济作物之一，安徽省茶树栽培历史悠久具有丰富的种质资源和遗传多样性。茶树的高度杂合性导致其种质资源收集、管理及保护等方面工作进展缓慢，并且长期保存管理成本高，因此提高安徽省茶树种质资源的管理质量和效率，构建核心种质迫在眉睫。本研究从安徽省6个主要产茶地区收集了573份有代表性的茶树，基于60对SSR分子标记进行系统发育关系、群体结构和主坐标分析。聚类结果显示，安徽省573份茶树被分为5个类群，这些类群分布地区与收集样品的地理位置相关，聚类结果与PCoA的分群结果也基本一致。最后，我们构建了一个由115份茶树品种组成的核心种质，占原始种质的20%。核心种质中观察到的等位基因数(Na)具有90.9%的保留率，并且核心种质和原始种质的香农信息指数(I)及其他遗传多样性参数之间没有显著性差异。所有代表性茶区均保留了部分代表性品种，其中黄山地区保留了39份茶树，占核心种质的33.9%；金寨县保留了10份茶树，占核心种质的8.9%。PCoA结果表明构建的核心种质均匀的分布在收集的安徽省茶树种质中，说明构建的核心种质能够代表安徽省茶树种质的遗传多样性。本研究对安徽省茶树种质资源的高效保存和利用具有一定的参考价值。

Abstract:

The tea plant [Camellia sinensis (L.) O. Kuntze] is an industrial crop in China.  The Anhui Province has a long history of tea cultivation and has a large resource of tea germplasm with abundant genetic diversity.  To reduce the cost of conservation and utilization of germplasm resources, a core collection needs to be constructed.  To this end, 573 representative tea accessions were collected from six major tea-producing areas in Anhui Province.  Based on 60 pairs of simple sequence repeat (SSR) markers, phylogenetic relationships, population structure and principal coordinate analysis (PCoA) were conducted.  Phylogenetic analysis indicated that the 573 tea individuals clustered into five groups were related to geographical location and were consistent with the results of the PCoA.  Finally, we constructed a core collection consisting of 115 tea individuals, accounting for 20% of the whole collection.  The 115 core collections were considered to have a 90.9% retention rate for the observed number of alleles (Na), and Shannon’s information index (I) of the core and whole collections were highly consistent.  Of these, 39 individuals were preserved in the Huangshan area, accounting for 33.9% of the core collection, while only 10 individuals were reserved in the Jinzhai County, accounting for 8.9% of the core set.  PCoA of the accessions in the tea plant core collection exhibited a pattern nearly identical to that of the accessions in the entire collection, further supporting the broad representation of the core germplasm in Anhui Province.  The results demonstrated that the core collection could represent the genetic diversity of the original collection.  Our present work is valuable for the high-efficiency conservation and utilization of tea plant germplasms in Anhui Province

Key words: tea plant ,  core collection ,  genetic diversity ,  SSR markers