SlTPP4增强盐胁迫下根的形态发育参与ABA-介导的番茄耐盐性

doi:10.1016/j.jia.2023.07.015

摘要/Abstract

摘要：

提高作物的耐盐性已是当前作物育种研究的主要方向之一，而植物根系在抵御高盐胁迫中起着重要作用。在本研究中，我们发现番茄海藻糖-6-磷酸磷酸酶基因（SlTPP4）主要在根系中表达，且其表达受脱落酸（ABA）和盐胁迫的诱导。进一步功能研究发现在番茄中超表达SlTPP4可以增强植株对盐胁迫的耐受性，表现出更好的生长态势。对其耐盐机制研究表明SlTPP4超表达株系中糖代谢途径以及盐胁迫相关基因的表达增强，此外，ABA合成途径相关基因的表达也升高，同时ABA信号转导途径相关基因的表达也发生了显著变化。进一步的深入解析发现SlTPP4能调控盐胁迫下根内皮层凯氏带的发育，减少有害离子非质外体途径的摄入进而提高植物对盐胁迫的耐受性。综上，本研究初步探明了SlTPP4介导耐盐性的生物学功能及机制，为耐盐番茄的育种提供了理论支撑。

Abstract:

Salinity tolerance is an important physiological index for crop breeding. Roots are typically the first plant tissue to withstand salt stress. In this study, we found that the tomato (Solanum lycopersicum) trehalose-6-phosphate phosphatase (SlTPP4) gene is induced by abscisic acid (ABA) and salt, and is mainly expressed in roots. Overexpression of SlTPP4 in tomato enhanced tolerance to salt stress, resulting in better growth performance. Under saline conditions, SlTPP4 overexpression plants demonstrated enhanced sucrose metabolism, as well as increased expression of genes related to salt tolerance. At the same time, expression of genes related to ABA biosynthesis and signal transduction was enhanced or altered, respectively. In-depth exploration demonstrated that SlTPP4 enhances Casparian band development in roots to restrict the intake of Na⁺. Our study thus clarifies the mechanism of SlTPP4-mediated salt tolerance, which will be of great importance for the breeding of salt-tolerant tomato crops.

Key words: trehalose-6-phosphate phosphatase (TPP) , salt tolerance , root , ABA , tomato (Solanum lycopersicum)

DU Dan, HU Xin, SONG Xiao-mei, XIA Xiao-jiao, SUN Zhen-yu, LANG Min, PAN Yang-lu, ZHENG Yu, PAN Yu. SlTPP4增强盐胁迫下根的形态发育参与ABA-介导的番茄耐盐性[J]. Journal of Integrative Agriculture, 2023, 22(8): 2384-2396.

DU Dan, HU Xin, SONG Xiao-mei, XIA Xiao-jiao, SUN Zhen-yu, LANG Min, PAN Yang-lu, ZHENG Yu, PAN Yu. SlTPP4 participates in ABA-mediated salt tolerance by enhancing root architecture in tomato[J]. Journal of Integrative Agriculture, 2023, 22(8): 2384-2396.

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