全基因组和候选基因关联关联分析鉴定BnPAP17在油菜有机磷利用中的功能

doi:10.1016/j.jia.2023.05.002

摘要/Abstract

摘要：

磷（P）是活体植物必不可少的重要元素。缺磷是制约世界油菜产量的关键因素之一。作为植物最重要的器官，根的形态在磷的吸收中起着关键作用。为了研究低磷有效性下根形态的遗传变异性，我们通过全基因组关联研究（GWAS）、连锁作图和候选基因关联研究（CGAS）对跟的形态性状相关的基因结构进行了剖析。在405份油菜自然群体中，共检测到52个重要的标记位点与磷胁迫下的根系形态性状相关。其中，在低磷胁迫下的侧根数（LRN）和根干重（RDW）性状位点中共同检测到紫色酸性磷酸酶基因-BnPAP17。在低磷胁迫下，高效磷品种的地上部组织中BnPAP17的表达比根组织和低效磷品种的表达增加。此外，还检测到BnPAP17^Hap3单倍型在油菜磷高效育种中受到了选择。过量表达该单倍型能显著促进油菜的地上部和根部生长，增强油菜对低磷胁迫的耐受性和对有机磷的利用。综上所述，这些发现增加了我们对BnPAP17在介导油菜低磷胁迫中的分子机制的认识。

Abstract:

Phosphorus (P) is essential for living plants, and P deficiency is one of the key factors limiting the yield in rapeseed production worldwide. As the most important organ for plants, root morphology traits (RMTs) play a key role in P absorption. To investigate the genetic variability of RMT under low P availability, we dissected the genetic structure of RMTs by genome-wide association studies (GWAS), linkage mapping and candidate gene association studies (CGAS). A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape accessions. The purple acid phosphatase gene BnPAP17 was found to control the lateral root number (LRN) and root dry weight (RDW) under low P stress. The expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P stress. Moreover, the haplotype of BnPAP17^Hap3 was detected for the selective breeding of P efficiency in oilseed rape. Over-expression of the BnPAP17^Hap3 could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus (Po) utilization in oilseed rape. Collectively, these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.

Key words: genome-wide association studies (GWAS) , root morphology traits (RMTs) , organic phosphorus (Po) , oilseed rape , BnPAP17

. 全基因组和候选基因关联关联分析鉴定BnPAP17在油菜有机磷利用中的功能[J]. Journal of Integrative Agriculture, 2024, 23(4): 1134-1149.

Ping Xu, Hao Li, Haiyuan Li, Ge Zhao, Shengjie Dai, Xiaoyu Cui, Zhenning Liu, Lei Shi, Xiaohua Wang.

Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape [J]. Journal of Integrative Agriculture, 2024, 23(4): 1134-1149.

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