Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (10): 3051-3066.DOI: 10.1016/j.jia.2022.07.045

所属专题： 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe

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JIA-2021-1486 在长期使用有机肥的高肥力土壤上土著AM真菌依然能够引起土壤磷的消耗

  

  • 收稿日期:2021-08-26 接受日期:2021-11-25 出版日期:2022-10-01 发布日期:2021-11-25

Indigenous arbuscular mycorrhizal fungi play a role in phosphorus depletion in organic manure amended high fertility soil

HUO Wei-ge1, CHAI Xiao-fen1, WANG Xi-he2, William David BATCHELOR3, Arjun KAFLE4, FENG Gu1   

  1. 1 College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R.China

    2 Research Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China   

    3 Biosystems Engineering Department, Auburn University, AL 36849, USA

    4 Department of Crop and Soil Sciences, North Carolina State University, NC 27695, USA

  • Received:2021-08-26 Accepted:2021-11-25 Online:2022-10-01 Published:2021-11-25
  • About author:HUO Wei-ge, E-mail: 18813119206@163.com; Correspondence FENG Gu, Tel: +86-10-62733885, E-mail: fenggu@cau.edu.cn
  • Supported by:
    This study was financially supported by the Beijing Natural Science Foundation, China (6202015), the National Natural Science Foundation of China (U1703232) and the Hatch Project (ALA014-1-16016) funded by the National Institute of Food and Agriculture, US Department of Agriculture.

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摘要： 集约化农业生产的土壤中含有丰富的AM真菌种类和孢子数目。以往的研究表明，在低磷条件下AM真菌能够提高作物的磷吸收，但在高磷土壤中AM真菌是否依然发挥着作用并不清楚。在本文中，我们原位研究了长期高磷肥投入的农田中，土著AM真菌是否对P的利用依然有贡献。我们设计了菌丝室装置，通过在PVC管两端分别密封不同孔径的膜（30 或 0.45 µm），允许或阻止菌丝穿透尼龙膜，进入菌丝室，并阻止棉花根系的进入。我们用土壤速效磷（Olsen-P）的耗竭来表征土著AMF对磷的吸收。结果表明，土著AMF能够介导磷的耗竭和微生物量磷（MBP）的周转，并且在高磷条件下（Olsen-P: 78.29 mg kg-1），速效磷的耗竭和MBP的周转率最大；不同施肥处理的棉花根内定殖着特有的AM真菌群落，且Glomus 和 Paraglomus占主导地位，暗示了长期的施肥能够驯化AM真菌群落。在本研究结果中，我们得出了即使在高磷条件下，土著AM真菌在土壤磷的耗竭和周转中依然发挥着重要作用。

Abstract:

The species richness and propagule number of arbuscular mycorrhizal fungi (AMF) are high in intensively-managed agricultural soils.  Past research has shown that AMF improve crop phosphorus (P) uptake under low soil P conditions, however it is unclear if AMF play a role in high Olsen-P soils.  In this study, we investigated whether native fungal benefits exist under high P input field conditions in-situ and contribute to P utilization.  We installed in-grow tubes which were sealed with different membrane pore sizes (30 or 0.45 µm) to allow or prevent AMF hyphae access to the hyphal compartment and prevent cotton roots from penetrating the chamber.  We used the depletion of soil available P (Olsen-P) in the hyphae accessed compartment to indicate P uptake by the native AMF community.  Our results showed that the native AMF mediated P depletion and microbial biomass P (MBP) turnover and caused the largest Olsen-P depletion ratio and MBP turnover ratio in the high P treatments (Olsen-P: 78.29 mg kg–1).  The cotton roots in each fertilization regime were colonized by a unique AMF community and Glomus and Paraglomus were the dominant genera, implying the long-term fertilization regimes domesticated the AMF community.  We conclude that native AMF caused the P depletion and P turnover even under high soil Olsen-P conditions.

Key words: arbuscular mycorrhizal fungi , phosphorus depletion , high P soil , Gossypium spp , indigenous community , mesh cores