Nudix水解酶家族在葡萄果实发育和逆境响应中的多种生理功能分析

doi:10.1016/S2095-3119(20)63490-6

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (1): 91-112.DOI: 10.1016/S2095-3119(20)63490-6

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

Nudix水解酶家族在葡萄果实发育和逆境响应中的多种生理功能分析

收稿日期:2020-07-02 接受日期:2020-11-16 出版日期:2022-01-01 发布日期:2022-01-01

Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine

WANG Pei-pei^{1, 2*}, WANG Zhao-ke^1*, GUAN Le², Muhammad Salman HAIDER², Maazullah NASIM², YUAN Yong-bing³, LIU Geng-sen³, LENG Xiang-peng^{1, 3}

¹ Institute of Grape Science and Engineering, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China
² College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
³ Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2020-07-02 Accepted:2020-11-16 Online:2022-01-01 Published:2022-01-01
About author:0 Correspondence LENG Xiang-peng, E-mail: lengpeng2008@ 163.com * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Research and Development Program of China (2019YFD1001405-02), the National Natural Science Foundation of China (NSFC; 32002005 and 31801809), the China Postdoctoral Science Foundation (2019M661866), the Breeding Plan of Shandong Provincial Qingchuang Research Team (2019), and the High-level Scientific Research Foundation of Qingdao Agricultural University, China (665/1118011 and 665/1119002).

摘要/Abstract

摘要：

Nudix水解酶家族普遍存在于真核和原核生物中，能够水解多种有机焦磷酸盐。尽管Nudix水解酶家族能够清除和调节细胞内产生的有害代谢产物，应答多种非生物和生物胁迫，但葡萄中的Nudix水解酶的功能还未见报道。本研究通过生物信息学分析，在葡萄中鉴定了25个葡萄VvNUDX基因家族成员，并根据其对底物的偏好性将其分为8个亚家族。VvNUDX基因家族在进化过程中存在串联复制和片段复制两种方式。为了研究VvNUDX基因在葡萄生长发育以及胁迫应该过程中的功能，我们利用NCBI公共数据库资源分析这些基因的表达模式。在不同葡萄组织中的时空表达分析表明，VvNUDX基因可能涉及多个生长发育过程。转录组数据和定量PCR分别表明，有10个VvNUDX基因在葡萄果实发育过程中特异表达，表明VvNUDX基因可能在葡萄果实发育过程中扮演重要角色。系统发育和表达分析表明，VvNUDX1和VvNUDX3可能参与葡萄萜类化合物的生物合成。多个ADP-核糖/NADH亚组的VvNUDX基因可以应答多种非生物和生物胁迫，表明VvNUDX基因参与葡萄对非生物和生物胁迫的解毒过程，并调节疾病免疫和抗性途径。相关研究结果为有助于进一步解析VvNUDX基因在葡萄果实发育和胁迫应答网络中的生理功能。

Abstract: Nudix hydrolases are widely distributed across all classes of organisms and provide the potential capacity to hydrolyze a wide range of organic pyrophosphates. Although Nudix hydrolases are involved in plant detoxification processes in response to abiotic and biotic stresses, the biological functions of Nudix hydrolases remain largely unclear in grapevine. In the present study, a total of 25 putative grapevine Nudix hydrolases (VvNUDXs) were identified by bioinformatics analysis and classified into eight subfamilies based to their preferred substrates. Both tandem and segmental duplications were responsible for the evolution and expansion of the NUDX gene family in grapevine. To investigate the regulatory roles of VvNUDX genes during growth and development, as well as in response to abiotic and biotic stresses in grapevine, the expression patterns were revealed in publicly available microarray data. The spatial and temporal expression patterns of the VvNUDX genes indicated that they might play important roles in multiple developmental processes. Transcriptome and qRT-PCR analyses showed that ten VvNUDX genes were specifically expressed in grapevine berries, suggesting potential roles in grapevine berry development. Expression and phylogenetic analyses demonstrated that VvNUDX1 and VvNUDX3 might be involved in terpenoid biosynthesis in grapevine. Furthermore, most VvNUDX genes active toward the ADP-ribose/NADH showed different patterns in response to various abiotic and biotic stresses, such as salinity and drought, as well as different types of biotic treatments, such as Erysiphe necator, Bois Noir phytoplasma and leaf-roll-associated virus-3 (GLRaV-3). These results indicated that VvNUDX genes were associated with plant detoxification processes in response to abiotic and biotic stresses, and regulate the disease immunity and resistance pathways. The information obtained here may provide good opportunities to explore the physiological functions of VvNUDX genes in berry development and stress response networks in grapevine.

Key words: grapevine , NUDX , gene expression , berry development , stress response

. Nudix水解酶家族在葡萄果实发育和逆境响应中的多种生理功能分析[J]. Journal of Integrative Agriculture, 2022, 21(1): 91-112.

WANG Pei-pei, WANG Zhao-ke, GUAN Le, Muhammad Salman HAIDER, Maazullah NASIM, YUAN Yong-bing, LIU Geng-sen, LENG Xiang-peng. Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine[J]. Journal of Integrative Agriculture, 2022, 21(1): 91-112.

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Nudix水解酶家族在葡萄果实发育和逆境响应中的多种生理功能分析

Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine

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