JIA-2021-1452 TaABI19 正调控小麦籽粒发育

doi:10.1016/j.jia.2022.08.049

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (1): 41-51.DOI: 10.1016/j.jia.2022.08.049

JIA-2021-1452 TaABI19 正调控小麦籽粒发育

收稿日期:2021-08-20 修回日期:2021-11-29 出版日期:2023-01-20 发布日期:2021-11-29

TaABI19 positively regulates grain development in wheat

LIU Yun-chuan^{1, 2}, WANG Xiao-lu^{1, 3}, HAO Chen-yang¹, IRSHAD Ahsan⁴, LI Tian¹, LIU Hong-xia¹, HOU Jian¹, ZHANG Xue-yong¹

¹ National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
² State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China
³ Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China
⁴ National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2021-08-20 Revised:2021-11-29 Online:2023-01-20 Published:2021-11-29
About author:LIU Yun-chuan, E-mail: liuyunchuan1991@163.com
Supported by:
This work was supported by the the Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Agricultural Sciences (Y2017PT39).

摘要/Abstract

摘要： 淀粉是谷物胚乳中最重要的成分，其合成受到多种转录因子的调控。然而，这些转录因子在谷物之间是否具有保守功能尚不清楚。本研究依据玉米(Zea mays L.)的同源基因，克隆了属于一个B3家族的转录因子，命名为TaABI19。DNA和蛋白质序列比对分析，发现ABI19在玉米和小麦中具有保守性。TaABI19在小麦幼穗和发育的籽粒中高表达，编码一个核定位的转录激活子。EMS诱变获得的taabi19-b1突变体表现为淀粉合成下调、粒长变短和千粒重降低。此外，我们还发现TaABI19能够结合到TaPBF亚基因组同源基因的启动子上并增强其表达。单倍型关联表明，TaABI19-B1与千粒重显著相关。TaABI19-B1-Hap2和-Hap3在中国小麦育种中被正向选择。在现代栽培品种中，TaABI19的优异单倍型比例不足50%，表明TaABI19仍可作为标记辅助选择育种的目标位点，提高我国小麦产量。

Abstract: Starch is the most important component in endosperm, and its synthesis is regulated by multiple transcription factors (TFs) in cereals. However, whether the functions of these TFs are conserved or not among cereals unclear yet. Here, we cloned a B3 family TF, named as TaABI19 based on its orthologous in maize (Zea mays L.). Alignment of DNA and protein showed that ABI19 was conserved in maize and wheat (Triticum aestivum L.). We found that TaABI19 was highly expressed in young spike and developing grains and encoded a nucleus-localized transcriptional activator in wheat. The taabi19-b1 null mutants obtained by EMS performed down-regulation of starch synthesis, shorter grain length and lower thousand grain weight (TGW). Furthermore, we provided TaABI19 could bind to the promoters of TaPBF homology genes and enhance their expression. Haplotype association showed that TaABI19-B1 was significantly associated with TGW. We found that Hap2 and Hap3 were favored and underwent positive selection in China wheat breeding. Less than fifty percent in the modern cultivars conveying favored haplotypes indicates TaABI19 still can be considered as target loci for marker-assisted selection breeding to increase TGW in China.

Key words: common wheat , transcription factor , haplotype , thousand grain weight

. JIA-2021-1452 TaABI19 正调控小麦籽粒发育[J]. Journal of Integrative Agriculture, 2023, 22(1): 41-51.

LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong. TaABI19 positively regulates grain development in wheat[J]. Journal of Integrative Agriculture, 2023, 22(1): 41-51.

参考文献

Bai A N, Lu X D, Li D Q, Liu J X, Liu C M. 2016. NF-YB1-regulated expression of sucrose transporters in aleurone facilitates sugar loading to rice endosperm. Cell Research, 26, 384–388.
Bello B K, Hou Y X, Zhao J, Jiao G A, Wu Y W, Li Z Y, Wang Y F, Tong X H, Wang W, Yuan W Y, Wei X J, Zhang J. 2019. NF-YB1-YC12-bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.). Plant Biotechnology Journal, 17, 1222–1235.
Brinton J, Uauy C. 2019. A reductionist approach to dissecting grain weight and yield in wheat. Journal of Integrative Plant Biology, 61, 337–358.
Chen J, Yi Q, Cao Y, Wei B, Zheng L J, Xiao Q L, Xie Y, Gu Y, Li Y P, Huang H H, Wang Y B, Hou X B, Long T D, Zhang J J, Liu H M, Liu Y H, Yu G W, Huang Y B. 2016. ZmbZIP91 regulates expression of starch synthesis-related genes by binding to ACTCAT elements in their promoters. Journal of Experimental Botany, 67, 1327–1338.
Chia T, Chirico M, King R, Ramirez-Gonzalez R, Saccomanno B, Seung D, Simmonds J, Trick M, Uauy C, Verhoeven T, Trafford K. 2020. A carbohydrate-binding protein, B-GRANULE CONTENT 1, influences starch granule size distribution in a dose-dependent manner in polyploid wheat. Journal of Experimental Botany, 71, 105–115.
Dai D W, Ma Z Y, Song R T. 2021. Maize endosperm development. Journal of Integrative Plant Biology, 63, 613–627.
Deng Y T, Wang J C, Zhang Z Y, Wu Y R. 2020. Transactivation of Sus1 and Sus2 by Opaque2 is an essential supplement to sucrose synthase-mediated endosperm filling in maize. Plant Biotechnology Journal, 18, 1897–1907.
Fan Y F, Li Y B. 2019. Molecular, cellular and Yin-Yang regulation of grain size and number in rice. Molecular Breeding, 39, 163.
Feng F, Qi W, Lv Y, Yan S, Xu L, Yang W, Yuan Y, Chen Y, Zhao H, Song R. 2018. OPAQUE11 is a central hub of the regulatory network for maize endosperm development and nutrient metabolism. The Plant Cell, 30, 375–396.
Gao Y J, An K X, Guo W W, Chen Y M, Zhang R J, Zhang X, Chang S Y, Rossi V, Jin F M, Cao X Y, Xin M M, Peng H R, Hu Z R, Guo W L, Du J K, Ni Z F, Sun Q X, Yao Y Y. 2021. The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality. The Plant Cell, 33, 603–622.
Hawkins E, Chen J, Watson-Lazowski A, Ahn-Jarvis J, Barclay J E, Fahy B, Hartley M, Warren F J, Seung D. 2021. STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endosperm. New Phytologist, 230, 2371–2386.
Hellens R P, Allan A C, Friel E N, Bolitho K, Grafton K, Templeton M D, Karunairetnam S, Gleave A P, Laing W A. 2005. Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods, 1, 13.
Hou J, Jiang Q Y, Hao C Y, Wang Y M, Zhang H N, Zhang X Y. 2014. Global selection on sucrose synthase haplotypes during a century of wheat breeding. Plant Physiology, 164, 1918–1929.
IWGSC (International Wheat Genome Sequencing Consortium). 2018. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science, 361, eaar7191.
James M G, Denyer K, Myers A M. 2003. Starch synthesis in the cereal endosperm. Current Opinion in Plant Biology, 6, 215–222.
Jeon J S, Ryoo N, Hahn T R, Walia H, Nakamura Y. 2010. Starch biosynthesis in cereal endosperm. Plant Physiology and Biochemistry, 48, 383–392.
Kuchel H, Williams K J, Langridge P, Eagles H A, Jefferies S P. 2007. Genetic dissection of grain yield in bread wheat. I. QTL analysis. Theoretical and Applied Genetics, 115, 1029–1041.
Liu H, Li H F, Hao C Y, Wang K, Wang Y M, Qin L, An D G, Li T, Zhang X Y. 2019. TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.). Plant Biotechnol Journal, 18, 1330–1342.
Liu Y C, Hou J, Wang X L, Li T, Majeed U, Hao C, Zhang X Y. 2020. The NAC transcription factor NAC019-A1 is a negative regulator of starch synthesis in wheat developing endosperm. Journal of Experimental Botany, 71, 5794–5807.
Maphosa L, Langridge P, Taylor H, Parent B, Emebiri L C, Kuchel H, Reynolds M P, Chalmers K J, Okada A, Edwards J, Mather D E. 2014. Genetic control of grain yield and grain physical characteristics in a bread wheat population grown under a range of environmental conditions. Theoretical and Applied Genetics, 127, 1607–1624.
Monke G, Altschmied L, Tewes A, Reidt W, Mock H P, Baumlein H, Conrad U. 2004. Seed-specific transcription factors ABI3 and FUS3: Molecular interaction with DNA. Planta, 219, 158–166.
Olsen O A, Linnestad C, Nichols S E. 1999. Developmental biology of the cereal endosperm. Trends in Plant Science, 4, 253–257.
Peng L T, Shi Z Y, Li L, Shen G Z, Zhang J L. 2007. Ectopic expression of OsLFL1 in rice represses Ehd1 by binding on its promoter. Biochemical and Biophysical Research Communications, 360, 251–256.
Ramirez-Gonzalez R H, Borrill P, Lang D, Harrington S A, Brinton J, Venturini L, Davey M, Jacobs J, van Ex F, Pasha A, Khedikar Y, Robinson S J, Cory A T, Florio T, Concia L, Juery C, Schoonbeek H, Steuernagel B, Xiang D, Ridout C J, et al. 2018. The transcriptional landscape of polyploid wheat. Science, 361, eaar6089.
Schmidt R J, Burr F A, Aukerman M J, Burr B. 1990. Maize regulatory gene opaque-2 encodes a protein with a “leucine-zipper” motif that binds to zein DNA. Proceedings of the National Academy of Sciences of Sciences of the United States, 87, 46–50.
Song Y H, Luo G B, Shen L S, Yu K, Yang W L, Li X, Sun J Z, Zhan K H, Cui D Q, Liu D C, Zhang A M. 2020. TubZIP28, a novel bZIP family transcription factor from Triticum urartu, and TabZIP28, its homologue from Triticum aestivum, enhance starch synthesis in wheat. New Phytologist, 226, 1384–1398.
Su Z Q, Hao C Y, Wang L, Dong Y C, Zhang X Y. 2011. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 122, 211–223.
Suzuki M, McCarty D R. 2008. Functional symmetry of the B3 network controlling seed development. Current Opinion in Plant Biology, 11, 548–553.
Vicente-Carbajosa J, Moose S P, Parsons R L, Schmidt R J. 1997. A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2. Proceedings of the National Academy of Sciences of Sciences of the United States, 94, 7685–7690.
Wang J, Chen Z C, Zhang Q, Meng S S, Wei C X. 2020. The NAC transcription factors OsNAC20 and OsNAC26 regulate starch and storage protein synthesis. Plant Physiology, 184, 1775–1791.
Wang J C, Xu H, Zhu Y, Liu Q Q, Cai X L. 2013. OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm. Journal of Experimental Botany, 64, 3453–3466.
Wang W, Pan Q L, Tian B, He F, Chen Y Y, Bai G H, Akhunova A, Trick H N, Akhunov E. 2019. Gene editing of the wheat homologs of TONNEAU1-recruiting motif encoding gene affects grain shape and weight in wheat. The Plant Journal, 100, 251–264.
Wang Y M, Hou J, Liu H, Li T, Wang K, Hao C Y, Liu H X, Zhang X Y. 2019. TaBT1, affecting starch synthesis and thousand kernel weight, underwent strong selection during wheat improvement. Journal of Experimental Botany, 70, 1497–1511.
Xiong Y F, Ren Y, Li W, Wu F S, Yan W J, Huang X L, Yao J L. 2019. NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice. Journal of Experimental Botany, 70, 3765–3780.
Xu J J, Zhang X F, Xue H W. 2016. Rice aleurone layer specific OsNF-YB1 regulates grain filling and endosperm development by interacting with an ERF transcription factor. Journal of Experimental Botany, 67, 6399–6411.
Yang T, Guo L X, Ji C, Wang H H, Wang J C, Zheng X X, Xiao Q, Wu Y R. 2021. The B3 domain-containing transcription factor ZmABI19 coordinates expression of key factors required for maize seed development and grain filling. The Plant Cell, 33, 104–128.
Zhan J P, Li G S, Ryu C H, Ma C, Zhang S S, Lloyd A, Hunter B G, Larkins B A, Drews G N, Wang X F, Yadegari R. 2018. Opaque-2 regulates a complex gene network associated with cell differentiation and storage functions of maize endosperm. The Plant Cell, 30, 2425–2446.
Zhang Z Y, Dong J Q, Ji C, Wu Y R, Messing J. 2019. NAC-type transcription factors regulate accumulation of starch and protein in maize seeds. Proceedings of the National Academy of Sciences of Sciences of the United States, 116, 11223–11228.
Zhang Z Y, Zheng X X, Yang J, Messing J, Wu Y R. 2016. Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis. Proceedings of the National Academy of Sciences of Sciences of the United States, 113, 10842–10847.
Zheng X X, Li Q, Li C S, An D, Xiao Q, Wang W Q, Wu Y R. 2019. Intra-kernel reallocation of proteins in maize depends on VP1-mediated scutellum development and nutrient assimilation. The Plant Cell, 31, 2613–2635.

JIA-2021-1452 TaABI19 正调控小麦籽粒发育

TaABI19 positively regulates grain development in wheat

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