Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (12): 3035-3044.DOI: 10.1016/S2095-3119(20)63237-3

所属专题: 昆虫合辑Plant Protection—Entomolgy 昆虫生防和生态合辑Insect Biocontrol and Ecology

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  • 收稿日期:2020-03-26 出版日期:2020-12-01 发布日期:2020-11-19

Attraction of bruchid beetles Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) to host plant volatiles

WANG Hong-min1*, BAI Peng-hua2, 3*, ZHANG Jing2, ZHANG Xue-min2, HUI Qin2, ZHENG Hai-xia2, ZHANG Xian-hong2 
  

  1. 1 College of Agricultural Economics and Management, Shanxi Agricultural University, Taigu 030801, P.R.China
    2 College of Plant Protection, Shanxi Agricultural University, Taigu 030801, P.R.China
    3 Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, P.R.China
  • Received:2020-03-26 Online:2020-12-01 Published:2020-11-19
  • Contact: Correspondence ZHANG Xian-hong, E-mail: zxh6288@126.com
  • About author:* These authors contributed equally to this study.
  • Supported by:
    This research was funded by the Shanxi Provincial Key Research and Development Project, China (201803D221004-8), the earmarked fund for China Agriculture Research System (CARS-08-G10), the Research and Demonstration of Key Green Technology for High Quality of Coarse Cereals in Northwest Shanxi (201703D211002-8) and the Shanxi Province Science Foundation for Youths (201801D221305).

Abstract:

Host-plant volatiles play an important role as cues for herbivores in search of resources, mates and oviposition sites in complex environments.  Plant volatile-based attractants can be developed for pest monitoring and control.  Previously, we indicated that mated female adults of Callosobruchus chinensis showed choice preference behavior toward 2-hexenal and benzaldehyde.  Our objective here was to investigate the synergistic effect of host-derived attractive volatiles in attracting C. chinensis under laboratory and field conditions in Shanxi Province, China.  We hypothesized that the ratio and concentration of volatiles derived from Vigna radiata play critical roles for C. chinensis in locating this host.  Therefore, we collected and identified the volatiles of mungbean by using headspace collection and GC-MS.  The effectiveness of different ratios and concentrations of two compounds (2-hexenal and benzaldehyde) that elicit C. chinensis searching behavior were examined in Y-tube olfactometer assays.  The combination of 300 μg μL–1 2-hexenal and 180 μg μL–1 benzaldehyde loadings exhibited a synergistic effect on attracting C. chinensis (82.35%).  Compared to control traps, the adults were significantly attracted to traps baited with blends, and more attraction to females than males was found for blend traps in the field experiments.  Our results suggest that blends of this specific concentration and ratio of benzaldehyde and 2-hexenal can be used in traps as attractants for C. chinensis monitoring and control in the field.

Key words: Callosobruchus chinensis ,  plant volatiles ,  synergistic effect ,  olfactometer ,  attractant