Cloning and functional characterization of two peptidoglycan recognition protein isoforms (PGRP-LC) in Bactrocera dorsalis (Diptera: Tephritidae)

doi:10.1016/S2095-3119(20)63202-6

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (12): 3025-3034.DOI: 10.1016/S2095-3119(20)63202-6

所属专题：昆虫合辑Plant Protection—Entomolgy；昆虫分子生物学合辑Insect Molecular Biology；昆虫和植物互作合辑Insect and Plant Interact

收稿日期:2020-12-30 出版日期:2020-12-01 发布日期:2020-11-19

Cloning and functional characterization of two peptidoglycan recognition protein isoforms (PGRP-LC) in Bactrocera dorsalis (Diptera: Tephritidae)

WEI Dong^{1, 2}, WANG Zhe^{1, 2}, XU Hui-qian^{1, 2}, NIU Jin-zhi^{1, 2}, WANG Jin-jun^{1, 2}

¹ Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P.R.China
² State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China

Received:2020-12-30 Online:2020-12-01 Published:2020-11-19
Contact: Correspondence WANG Jin-jun, Tel: +86-23-68250255, Fax: +86-23-68251269, E-mail: wangjinjun@swu.edu.cn
Supported by:
This research was funded in part by the National Key Research and Development Program of China (2019YFD1002101), and the earmarked fund for the Modern Agro-industry (Citrus) Technology Research System of China (CARS-27).

摘要/Abstract

Abstract:

The innate immune system of insects is the front line of self-defense against pathogen invasion. Peptidoglycan recognition proteins (PGRPs) are important components and play key roles in insect immune systems by recognizing peptidoglycan (PGN) in bacterial cell walls. We characterized two isoforms of the PGRP-LC gene, BdPGRP-LCa and BdPGRP-LCb, from Bactrocera dorsalis (Hendel), an important fruit and vegetable pest worldwide. These two isoforms contain an open reading frames of 1 668 bp and 1 731 bp, encoding a protein of 555 and 576 amino acids, respectively. Quantitative real-time PCR results showed that both transcripts were prominently expressed in midgut and fat body of B. dorsalis adult. Inoculation of pathogens showed that both isoforms actively responded to Escherichia coli PGN. We also observed a light response to Staphylococcus aureus PGN. Upon Beauveria bassiana inoculation, the expression of BdPGRP-LCa was enhanced, but the expression of BdPGRP-LCb was suppressed. Suppression of both transcripts by RNA interference led to increased mortality of flies challenged by E. coli, indicating that the two isoforms are involved in sensing Gram-negative bacterial infections.

Key words: oriental fruit fly , innate immunity , PGRPs , Gram-negative bacterium

. [J]. Journal of Integrative Agriculture, 2020, 19(12): 3025-3034.

WEI Dong, WANG Zhe, XU Hui-qian, NIU Jin-zhi, WANG Jin-jun. Cloning and functional characterization of two peptidoglycan recognition protein isoforms (PGRP-LC) in Bactrocera dorsalis (Diptera: Tephritidae)[J]. Journal of Integrative Agriculture, 2020, 19(12): 3025-3034.

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Cloning and functional characterization of two peptidoglycan recognition protein isoforms (PGRP-LC) in Bactrocera dorsalis (Diptera: Tephritidae)

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