Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat

doi:10.1016/S2095-3119(20)63246-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (9): 2247-2256.DOI: 10.1016/S2095-3119(20)63246-4

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-02-04 出版日期:2020-09-01 发布日期:2020-08-04

Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat

CHI Zhuo-heng¹, WANG Yong-qing¹, DENG Qun-xian¹, ZHANG Hui¹, PAN Cui-ping¹, YANG Zhi-wu²

¹ College of Horticulture, Sichuan Agricultural University, Chengdu 611130, P.R.China
² Sichuan Academy of Forestry, Chengdu 610081, P.R.China

Received:2020-02-04 Online:2020-09-01 Published:2020-08-04
Contact: Correspondence WANG Yong-qing, E-mail: yqw14@sicau.edu.cn
About author:CHI Zhuo-heng, E-mail: chizhuoheng 0331@foxmail.com;
Supported by:
This work was financially supported by the Key Research Project of Science and Technology, Sichuan, China (2016NYZ0034).

摘要/Abstract

Abstract:

Flowering is an important process for the reproduction of higher plants. Up to this point, the studies on flowering have mostly focused on the model plant Arabidopsis thaliana, and the flowering mechanism of fruit trees remains mostly unknown. The diversity of the flowering time of loquat (Eriobotrya japonica Lindl.) makes it an ideal material to study the regulation of flowering. In this study, we first observed the inflorescence bud differentiation in two varieties of loquat that had different blooming times (cv. Dawuxing (E. japonica), that blooms in the fall and cv. Chunhua (E. japonica×Eriobotrya bengalensis Hook. f.) that blooms in the spring) and found that the starting time of inflorescence bud differentiation and the speed of inflorescence development were responsible for the difference in blooming times. The determination of endogenous phytohormones by high performance liquid chromatography (HPLC) indicated that abscisic acid (ABA), zeatin (ZT), and gibberellin (GA₃) promoted flowering in loquat, while indole-3-acetic acid (IAA) was mainly involved in inflorescence bud differentiation in Chunhua. A transcription level analysis illustrated that multiple flowering-related genes could respond to different signals, integrate to the TFL1, AP1 and FT genes, and then synergistically regulate flowering in loquat. Thus, this study provides a new insight into flowering regulation mechanisms in loquat.

Key words: loquat , inflorescence bud differentiation , flowering , endogenous hormones , gene expression

. [J]. Journal of Integrative Agriculture, 2020, 19(9): 2247-2256.

CHI Zhuo-heng, WANG Yong-qing, DENG Qun-xian, ZHANG Hui, PAN Cui-ping, YANG Zhi-wu. Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat[J]. Journal of Integrative Agriculture, 2020, 19(9): 2247-2256.

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Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat

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