Overexpression of StCYS1 gene enhances tolerance to salt stress in the transgenic potato (Solanum tuberosum L.) plant

doi:10.1016/S2095-3119(20)63262-2

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (9): 2239-2246.DOI: 10.1016/S2095-3119(20)63262-2

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-01-06 出版日期:2020-09-01 发布日期:2020-08-04

Overexpression of StCYS1 gene enhances tolerance to salt stress in the transgenic potato (Solanum tuberosum L.) plant

LIU Min-min¹, LI Ya-lun¹, LI Guang-cun², DONG Tian-tian¹, LIU Shi-yang¹, LIU Pei¹, WANG Qing-guo¹

¹ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
² Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing 100081, P.R.China

Received:2020-01-06 Online:2020-09-01 Published:2020-08-04
Contact: Correspondence LIU Pei, Tel/Fax: +86-538-8246021, E-mail: liupei83224@sdau.edu.cn; WANG Qing-guo, E-mail: wqgyyy@126.com
About author:LIU Min-min, E-mail: 530465553@qq.com;
Supported by:
This work was supported by the National Natural Science Foundation of China (31901752) and by a grant from the Potato Industry Innovation Team for Modern Agricultural Industry Technology System, Shandong Province, China (SDAIT-10-011-11).

摘要/Abstract

Abstract:

Salt stress seriously restricts the growth and yield of potatoes. Plant cystatins are vital players in biotic stress and development, however, their roles in salt stress resistance remain elusive. Here, we report that StCYS1 positively regulates salt tolerance in potato plants. An in vitro biochemical test demonstrated that StCYS1 is a bona fide cystatin. Overexpression of StCYS1 in both Escherichia coli and potato plants significantly increased their resistance to high salinity. Further analysis revealed that the transgenic plants accumulated more proline and chlorophyll under salt stress conditions. Moreover, the transgenic plants displayed higher H₂O₂ scavenging capability and cell membrane integrity compared with wild-type potato. These results demonstrate that StCYS1 is closely correlated with salt stress and its overaccumulation can substantially enhance salt stress resistance.

Key words: potato , salt stress , cystatin StCYS1 , overexpression

. [J]. Journal of Integrative Agriculture, 2020, 19(9): 2239-2246.

LIU Min-min, LI Ya-lun, LI Guang-cun, DONG Tian-tian, LIU Shi-yang, LIU Pei, WANG Qing-guo. Overexpression of StCYS1 gene enhances tolerance to salt stress in the transgenic potato (Solanum tuberosum L.) plant[J]. Journal of Integrative Agriculture, 2020, 19(9): 2239-2246.

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Overexpression of StCYS1 gene enhances tolerance to salt stress in the transgenic potato (Solanum tuberosum L.) plant

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