Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)

doi:10.1016/S2095-3119(19)62684-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (6): 1565-1576.DOI: 10.1016/S2095-3119(19)62684-5

收稿日期:2019-02-02 出版日期:2020-06-01 发布日期:2020-04-26

Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)

LIU Lei^1*, FAN Wen-qi^{1, 2*}, LIU Fu-xia^1*, YI Xin¹, TANG Tang¹, ZHOU Ying^{1, 2}, TANG Zi-wei¹, CHEN Gui-min¹, ZHAO Xiang-xiang¹

¹ Jiangsu Key Laboratory for Eco-agriculture Biotechnology Around Hongze Lake/Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environment Protection/College of Life Science, Huaiyin Normal University, Huai’an 223300, P.R.China
² School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, P.R.China

Received:2019-02-02 Online:2020-06-01 Published:2020-04-26
Contact: Correspondence ZHAO Xiang-xiang, Tel: +86-517-83525885, E-mail: xxzhao2013@163.com
About author:LIU Lei, Tel: +86-517-83525778, E-mail: leiliu_cell@163.com; * These authors contributed equally to this study.
Supported by:
This work was funded by the National Natural Science Foundation of China (31470572 and 31701514) and the Qing Lan Project of Jiangsu Province, China (2016).

摘要/Abstract

Abstract:

Brassica napus cultivars have little or no primary dormancy; however, they are prone to secondary dormancy induction. Secondary dormant seeds can produce volunteer plants, which can result in genetic contamination, reduced quality and biosafety issues. However, information regarding the molecular mechanism underlying secondary dormancy is limited. The MOTHER OF FT AND TFL1 (MFT) gene, which is evolutionarily conserved in the plant kingdom, acts in a complex gene network in the seed dormancy or germination processes. In this study, we identified four B. napus genes that share high homology with AtMFT, named as BnaMFT. Analyses of cis-acting elements showed that BnaMFT promoters contain multiple seed-specific regulatory elements, and various stress- and hormone-responsive elements. Further experiments validated that BnaMFTs were specifically expressed during seed maturation and in the dry seed, with peaks at 35–42 days after pollination. BnaMFTs were not sufficient for primary dormancy; however, they were significantly enhanced by secondary dormancy induction with PEG6000 treatment. Moreover, BnaMFT transcripts were elevated by treatment with abscisic acid (ABA), which is known to be accumulated during secondary dormancy. These results collectively suggest that increased BnaMFT transcription levels are associated with secondary dormancy induction in an ABA-dependent manner in B. napus.

Key words: Brassica napus , DELAY OF GERMINATION 1 (DOG1) , MFT , primary dormancy , secondary dormancy

. [J]. Journal of Integrative Agriculture, 2020, 19(6): 1565-1576.

LIU Lei, FAN Wen-qi, LIU Fu-xia, YI Xin, TANG Tang, ZHOU Ying, TANG Zi-wei, CHEN Gui-min, ZHAO Xiang-xiang. Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)[J]. Journal of Integrative Agriculture, 2020, 19(6): 1565-1576.

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Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)

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