The sex peptide receptor in the Asian gypsy moth, Lymantria dispar, is involved in development and stress resistance

doi:10.1016/S2095-3119(20)63365-2

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (11): 2976-2985.DOI: 10.1016/S2095-3119(20)63365-2

所属专题：昆虫合辑Plant Protection—Entomolgy；害虫抗药性和毒理学合辑Pest Toxicology

收稿日期:2020-05-20 出版日期:2021-11-01 发布日期:2021-09-17

The sex peptide receptor in the Asian gypsy moth, Lymantria dispar, is involved in development and stress resistance

DU Hui, SUN Li-li, LIU Peng, CAO Chuan-wang

Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education, School of Forestry, Northeast Forestry University, Harbin 150040, P.R.Chin

Received:2020-05-20 Online:2021-11-01 Published:2021-09-17
Contact: Correspondence CAO Chuan-wang, Tel: +86-451-82191822, E-mail: chuanwangcao@nefu.edu.cn
About author:DU Hui, E-mail: 854894037@qq.com;
Supported by:
This work was supported by grants from the National Key R&D Program of China (2018YFC1200400), the National Natural Science Foundation of China (31570642), the Fundamental Research Funds for the Central Universities, China (2572019CG04) and the Heilongjiang Touyan Innovation Team Program, China (Tree Genetics and Breeding Innovation Team).

摘要/Abstract

摘要：

G蛋白偶联受体（GPCR）通过与异源三聚体G蛋白结合来调控下游基因。然而，大多数鳞翅目物种中性肽受体的功能未知。了解昆虫性肽受体生理功能对于开发新型杀虫剂作用靶标至关重要。本文研究了亚洲型舞毒蛾性肽受体（LdSPR）功能。舞毒蛾6龄幼虫LdSPR基因表达量最高，雌雄成虫之间的表达量存在显著性差异。LdSPR基因沉默后，舞毒蛾幼虫对高温、饥饿和氧化应激的敏感性增强，表明LdSPR基因增强了其抗逆性。这些研究结果丰富了我们对昆虫性肽受体功能的认识，有助于更好地了解其它昆虫中的G蛋白偶联受体家族成员，并为开发环境友好型农药鉴定新的作用靶标。

Abstract:

The G protein-coupled receptor (GPCR) regulates downstream genes by binding to a heterotrimeric G protein. However, the function of sex peptide receptor (SPR) in lepidopteran species is mostly unknown. Understanding the physiological functions of SPR in insects is essential for exploring new insecticidal targets. In the present study, the functions of an SPR in Lymantria dispar (Asian gypsy moth; LdSPR) were investigated. The expression of LdSPR was the highest in the 6th instar larval stage, and there was a large difference in expression between male and female adults. After LdSPR gene silencing, L.?dispar larvae showed increased sensitivity to high temperature, starvation, and oxidative stress, indicating that LdSPR enhances stress resistance. These results enrich our knowledge of the function of the insect SPRs, which will lead to a better understanding of other insect GPCR family members and the identification of new targets for the development of environmentally friendly pesticides.

Key words: Lymantria dispar , sex peptide receptor , RNAi , physiological function , stress resistance

. [J]. Journal of Integrative Agriculture, 2021, 20(11): 2976-2985.

DU Hui, SUN Li-li, LIU Peng, CAO Chuan-wang. The sex peptide receptor in the Asian gypsy moth, Lymantria dispar, is involved in development and stress resistance[J]. Journal of Integrative Agriculture, 2021, 20(11): 2976-2985.

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The sex peptide receptor in the Asian gypsy moth, Lymantria dispar, is involved in development and stress resistance

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