Transcriptome analysis reveals effects of red and blue lightemitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormones of potato (Solanum tuberosum L.) plantlets cultured in vitro

doi:10.1016/S2095-3119(20)63393-7

摘要/Abstract

摘要：

研究报道红、蓝光源显著影响植物幼苗生长。马铃薯是世界上重要的粮饲兼用作物。马铃薯组培苗培养在马铃薯生产中扮演重要角色。然而，从转录组水平上揭示红、蓝光源对马铃薯组培苗生长的影响研究较少。本研究的目的是借助转录组技术探索单色红光（RR）、单色蓝光（BB）和红蓝组合（RB）光谱处理的马铃薯组培苗的生长和生理反应。与对照RB相比，RR和BB处理的马铃薯组培苗分别被检测到有3150和814个差异表达基因。与对照相比，富集在“光合作用”和“光合天线蛋白”代谢通路上的差异表达基因分别被BB和RR处理所上调和下调表达，这可能与在上述两个处理中分别增加和降低的叶绿素荧光参数F_v/F_m, φPSⅡ,q_p和ETR有关。BB处理的马铃薯组培苗呈现出茎秆矮化、叶片较大，而RR处理则表现为茎秆伸长，叶片较小。这些显著的形态改变与马铃薯组培苗叶和茎器官中内源激素GAs，IAA 和CKs含量不同有关。此外，单色红、蓝LEDs光源引起“植物激素信号传导”通路上差异基因相反的表达模式与其激素含量不同有密切关系。本研究在转录组水平上揭示了马铃薯组培苗对红、蓝LEDs光源的不同响应，并从光谱特征方面有助于马铃薯组培苗快繁。

Abstract: Red and blue light illumination has been reported to significantly affect plantlet growth. Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production. However, few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level. The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red (RR), monochromatic blue (BB) as well as combined red and blue (RB) LEDs using the RNA-Seq technique. In total, 3 150 and 814 differentially expressed genes (DEGs) were detected in potato plantlets under RR and BB, respectively, compared to RB (used as control). Compared to the control, the DEGs enriched in “photosynthesis” and “photosynthesis-antenna proteins” metabolic pathways were up-regulated and down-regulated by BB and RR, respectively, which might be responsible for the increases and decreases of maximum quantum yield (F_v/F_m), photochemical quantum yield (φ_PSII), photochemical quenching (qP) and electron transfer rate (ETR) in BB and RR, respectively. Potato plantlets exhibited dwarfed stems and extended leaves under BB, whereas elongated stems and small leaves were induced under RR. These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin (GAs), indoleacetic acid (IAA) and cytokinins (CKs), as assessed in stems and leaves of potato plantlets. In addition, monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the “plant hormone signal transduction” metabolic pathway, which were closely related to the endogenous plant hormone levels in potato plantlets. Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.

Key words: potato (Solanum tuberosum L.) plantlets in vitro , red/blue LEDs light sources , RNA-seq, chlorophyll fluorescence , plant hormone

. [J]. Journal of Integrative Agriculture, 2021, 20(11): 2914-2931.

CHEN Li-li, WANG Hao-ying, GONG Xiao-chen, ZENG Zhao-hai, XUE Xu-zhang, HU Yue-gao. Transcriptome analysis reveals effects of red and blue lightemitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormones of potato (Solanum tuberosum L.) plantlets cultured in vitro[J]. Journal of Integrative Agriculture, 2021, 20(11): 2914-2931.

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