Genome-wide analysis of the SCPL gene family in grape (Vitis vinifera L.)

doi:10.1016/S2095-3119(20)63587-0

摘要/Abstract

摘要：

本研究从葡萄基因组中共鉴定出59个SCPL蛋白，并对该基因家族进行了染色体定位、序列比对、基因结构、系统进化，及保守结构域等生物信息学分析，该基因家族的鉴定严格按照SCPL结构域进行。系统进化分析结果表明，VvSCPL基因家族可被划分成3个亚家族，每个亚家族内的基因均具有高度保守的结构域。VvSCPL基因家族外显子数量介于1-19之间，表明葡萄SCPL基因存在较大的变异。转录组测序与qRT-PCR分析结果表明，VvSCPL基因的表达受干旱和淹水胁迫的诱导或抑制，表明该家族基因可能在应答非生物胁迫过程中发挥着一定的作用。本研究为深入了解葡萄VvSCPL基因的生理与生物学功能提供了有力参考。

Abstract:

Serine carboxypeptidase-like (SCPL) proteins are a group of acyltransferase enzymes that have important roles in plant growth, development, and stress responses. Although SCPL proteins have been studied in many plants, the biological functions of SCPL genes in grape are still unknown. In this study, 59 putative SCPL proteins were identified from the grape genome. A bioinformatics analysis, including chromosomal locations, exon/intron structures, phylogeny, cis-elements, and conserved motifs, was performed for the gene family. The phylogenetic analysis revealed that VvSCPL proteins could be classified into three groups, with the gene motifs in each group showing high similarity levels. The number of exons in the VvSCPL genes ranged from 1 to 19, suggesting significant variations among grape SCPL genes. The expression of the VvSCPL genes, as assessed by RNA sequencing (RNA-seq) and quantitative real-time PCR, showed that most VvSCPL genes responded to drought- and waterlogging-stress treatments, which indicated their roles in abiotic stress responses. The results provide useful information for further study of SCPL genes in grape.

Key words: serine carboxypeptidase-like (SCPL) protein , grape , phylogenetic relationships , abiotic stress , gene expression

. [J]. Journal of Integrative Agriculture, 2021, 20(10): 2666-2679.

WANG Xi-cheng, WU Wei-min, ZHOU Bei-bei, WANG Zhuang-wei, QIAN Ya-ming, WANG Bo, YAN Li-chun. Genome-wide analysis of the SCPL gene family in grape (Vitis vinifera L.)[J]. Journal of Integrative Agriculture, 2021, 20(10): 2666-2679.

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