Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (10): 2652-2665.DOI: 10.1016/S2095-3119(20)63381-0

所属专题: 园艺-分子生物合辑Horticulture — Genetics · Breeding

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  • 收稿日期:2020-04-09 出版日期:2021-10-01 发布日期:2021-08-09

Genome-wide analysis of the invertase genes in strawberry (Fragaria×ananassa)

YUAN Hua-zhao, PANG Fu-hua, CAI Wei-jian, CHEN Xiao-dong, ZHAO Mi-zhen, YU Hong-mei   

  1. Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, P.R.China
  • Received:2020-04-09 Online:2021-10-01 Published:2021-08-09
  • Contact: Correspondence YU Hong-mei, Tel: +86-25-84390219, Fax: +86-25-84391695, E-mail: yhmjsxh@163.com
  • About author:YUAN Hua-zhao, Tel: +86-25-84390219, E-mail: 544250348@qq.com;
  • Supported by:
    This work was funded by the Major Project for Breeding?New Varieties of Jiangsu Province, China (PZCZ201721), the National Horticulture Germplasm Resources Center, China (NHGRC2020-NH16), and the National Crop Germplasm Resources Protection of the Ministry of Agriculture and Rural Affairs of China (19200361).

摘要:

本文对八倍体栽培草莓INV家族基因进行了全面的分析,包括基因结构、染色体定位、保守结构域、基因进化和果实发育过程中的表达谱。我们的研究表明,多倍体事件导致INVs基因在栽培草莓基因组中大量扩增(几乎是三倍或四倍),这些扩增的INVs基因在草莓果实中表现为显性表达。超过一半的INVs转录产物由于可变剪切导致产生较短的编码框,同时具备较低的表达水平。前人的研究表明,CWINVs参与糖分的韧皮部卸载和库强度的调节。FaCWINV1在果实发育过程中表达量明显上调,在成熟果实中强烈表达。总糖含量与FaCWINV1的表达水平有显著的相关性。这些结果表明,FaCWINV1可能参与草莓果实中糖份的积累。综上所述,我们的研究结果将有助于进一步研究INVs在果实成熟调控中的作用。


Abstract:

Sugar is an important material basis in fruit development, and strawberry fruit flavour and sweetness largely depend on the sugar content and variety.  Invertases (INVs) play an important role in the regulation of sugar accumulation because they irreversibly catalyse the hydrolysis of sucrose into the corresponding nucleoside diphosphate-glucose, glucose or fructose in fruit.  In this work, we provided a comprehensive analysis of the INV gene family in octoploid strawberry (Fragaria×ananassa), including the gene structure, chromosomal locations, conserved domains, and gene evolution and expression profiles during strawberry fruit development.  Our study revealed that polyploid events resulted in the abundant amplification (almost three- or four-fold) of the INV gene in the F.×ananassa genome, and these amplified INV genes showed dominant expression in strawberry fruit.  More than half of the FaINVs transcripts with low expression had incomplete coding sequences by alternative splicing.  Previous studies have shown that cell wall invertases (CWINV) are involved in the regulation of phloem unloading and sink strength establishment.  The expression of FaCWINV1 was markedly upregulated during fruit development and strongly expressed in ripe fruit.  Moreover, a significant correlation was observed between the total sugar content and the FaCWINV1 expression level.  These findings suggest that FaCWINV1 may be involved in sugar accumulation in strawberry fruit.  Taken together, the results of our study will be beneficial for further research into the functions of INVs in the regulation of fruit ripening.

Key words: strawberry ,  sugar ,  invertases ,  fruit ripening