Receptor-like kinase OsASLRK regulates methylglyoxal response and content in rice

doi:10.1016/S2095-3119(20)63258-0

摘要/Abstract

摘要：

类受体蛋白激酶（Receptor-like kinases, RLKs）对于植物响应非生物胁迫至关重要。丙酮醛（Methylglyoxal, MG）作为一种细胞内源代谢产物，通常被认为是一种逆境信号分子。然而，关于RLK和MG之间的关系确知之甚少。本研究中，我们阐述了一个类受体蛋白激酶OsASLRK调控水稻MG响应和内源MG含量的功能。首先，我们发现OsASLRK基因启动子区域含有一个典型的MG反应元件（AAAAAAAA）。定量RT-PCR分析表明，OsASLRK转录水平的表达以时间和剂量依赖性方式显著受外源MG的诱导。GUS染色同样证实，外源MG处理可显著增强水稻根中OsASLRK的表达。遗传学分析表明，Osaslrk突变体表现出对外源MG敏感性增强，并且在外源MG处理下内源MG含量上升，而过表达OsASLRK的水稻植株表现出相反的表型。DAB染色，氧化清除酶活性和GSH含量测定表明，OsASLRK通过提高抗氧化酶活性和减轻膜损伤来调节MG敏感性和含量变化。因此，该研究结果为阐明水稻类受体蛋白激酶OsASLRK调控MG的功能提供了新证据。

Abstract:

Receptor-like kinases (RLKs) are essential for plant abiotic stress responses. Methylglyoxal (MG) is a cellular metabolite that is often considered to be a stress signal molecule. However, limited information is available about the relationship between RLKs and MG. Here, we addressed the function of a receptor-like kinase, OsASLRK, in the MG response and content in rice. A typical MG-responsive element (AAAAAAAA) exists in the promoter region of the OsASLRK gene. RT-qPCR analysis indicated that the transcript level of OsASLRK was significantly increased by exogenous MG in a time- and dosage-dependent fashion. GUS staining also confirmed that the expression of OsASLRK in rice root was enhanced by exogenous MG treatment. Genetic analysis suggested that the Osaslrk mutant displays increased sensitivity to MG and it showed higher endogenous MG content under exogenous MG treatments, while OsASLRK-overexpressing rice plants showed the opposite phenotypes. Diaminobenzidine (DAB) staining, scavenging enzyme activities and GSH content assays indicate that OsASLRK regulates MG sensitivity and content via the elevation of antioxidative enzyme activities and alleviation of membrane damage. Therefore, our results provide new evidence illustrating the roles that receptor-like kinase OsASLRK plays in MG regulation in rice.

Key words: receptor-like kinase (RLK) , OsASLRK , methylglyoxal (MG) , rice

. [J]. Journal of Integrative Agriculture, 2021, 20(7): 1731-1742.

LIN Fa-ming, LI Shen, WANG Ke, TIAN Hao-ran, GAO Jun-feng, DU Chang-qing. Receptor-like kinase OsASLRK regulates methylglyoxal response and content in rice[J]. Journal of Integrative Agriculture, 2021, 20(7): 1731-1742.

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