Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

doi:10.1016/S2095-3119(14)60872-8

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (7): 1229-1241.DOI: 10.1016/S2095-3119(14)60872-8

Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

ZHANG Fan, CHEN Xun-ji, WANG Jian-hua, ZHENG Jun

1、College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2、Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3、Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China

收稿日期:2014-07-15 出版日期:2015-07-03 发布日期:2015-07-06
通讯作者: ZHENG Jun, Tel: +86-10-82105863, Mobile: +86-13683230661, E-mail: zhengjun02@ caas.cn
作者简介:ZHANG Fan, Tel: +86-10-82105866, E-mail: zhangfan_cau@126.com
基金资助:
This work was supported by the National High Technology R&D Program of China (2012AA10A306), the National Natural Science Foundation of China (31330056) and the Xinjiang High-Tech Research Projects, China (201011109).

Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

ZHANG Fan, CHEN Xun-ji, WANG Jian-hua, ZHENG Jun

1、College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2、Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3、Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China

Received:2014-07-15 Online:2015-07-03 Published:2015-07-06
Contact: ZHENG Jun, Tel: +86-10-82105863, Mobile: +86-13683230661, E-mail: zhengjun02@ caas.cn
About author:ZHANG Fan, Tel: +86-10-82105866, E-mail: zhangfan_cau@126.com
Supported by:
This work was supported by the National High Technology R&D Program of China (2012AA10A306), the National Natural Science Foundation of China (31330056) and the Xinjiang High-Tech Research Projects, China (201011109).

摘要/Abstract

摘要： Sucrose non-fermenting-1 related protein kinase 2 (SnRK2) is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. A transient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde (MDA) content relative to the phenotypes observed in wild-type (WT) control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CS1. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.

关键词: maize , salt and drought stresses , ZmSnRK2.11 , SnRK2

Abstract: Sucrose non-fermenting-1 related protein kinase 2 (SnRK2) is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. A transient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde (MDA) content relative to the phenotypes observed in wild-type (WT) control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CS1. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.

Key words: maize , salt and drought stresses , ZmSnRK2.11 , SnRK2

ZHANG Fan, CHEN Xun-ji, WANG Jian-hua, ZHENG Jun. Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2015, 14(7): 1229-1241.

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Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

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