岩藻糖苷酶MoFco1调控稻瘟病菌侵染的机制研究

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (9): 3736-3745.DOI: 10.1016/j.jia.2025.06.004

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岩藻糖苷酶MoFco1调控稻瘟病菌侵染的机制研究

收稿日期:2025-02-19 修回日期:2025-06-02 接受日期:2025-04-30 出版日期:2026-09-20 发布日期:2026-08-10

A novel fucosylation-specific cell wall-degrading enzyme promotes Magnaporthe oryzae infection

Chang’an Ji, Zhao Hu, Yifang Zhang, Xia Song, Lei Su, Jintao Wang, Linxun Wu, Muxing Liu, Gang Li, Haifeng Zhang, Leiyun Yang, Xinyu Liu^#, Zhengguang Zhang^#

State Key Laboratory of Agricultural and Forestry Biosecurity, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China

Received:2025-02-19 Revised:2025-06-02 Accepted:2025-04-30 Online:2026-09-20 Published:2026-08-10
About author:Chang’an Ji, E-mail: 2019202011@njau.edu.cn; #Correspondence Zhengguang Zhang, E-mail: zhgzhang@njau.edu.cn; Xinyu Liu, E-mail: xinyuliu@njau.edu.cn
Supported by:
This research was supported by the National Key Research and Development Program of China (2022YFD1700200), the Young Elite Scientists Sponsorship Program by CAST (2022QNRC001), the Natural Science Foundation of China (NSFC) (32272496, 32293241 and 32293245). Virtual screening supported by the high-performance computing platform of Bioinformatics Center, Nanjing Agricultural University.

摘要/Abstract

摘要：

病原真菌与寄主互作时，病原物释放细胞壁降解酶降解植物细胞壁以促进侵染。木葡聚糖侧链的岩藻糖基化修饰是植物半纤维素中常见的糖基化形式之一，但稻瘟病菌是否通过分泌岩藻糖苷酶调控水稻木葡聚糖的岩藻糖基化水平，尚不清楚。有研究报道，禾谷镰刀菌α-L-岩藻糖苷酶FgFco1对豌豆木葡聚糖寡糖XXFG具有活性。本研究聚焦于FgFco1在稻瘟病菌中的同源蛋白MoFco1，探讨其在致病过程中的功能。通过序列比对和系统发育分析，我们发现稻瘟病菌中存在三个FgFco1同源基因，其中MoFco1与FgFco1最为相似，并在序列和结构上均高度保守。酶活检测表明，MoFco1可水解α-1,2连接的岩藻糖修饰寡糖，如2’-FL和XXFG。进一步通过定位观察发现MoFco1在侵染过程中分泌到质外体空间。进一步对MoFco1的功能进行解析，发现其调控稻瘟病菌的致病力，并且其功能依赖于其酶活。在此基础上，以AlphaFold3构建的MoFco1结构进行虚拟筛选，获得了化合物0989，采用微量热泳动技术明确0989与MoFco1结合关系，进一步实验证实，0989可显著抑制稻瘟病菌的致病力。综上，本研究揭示了MoFco1在稻瘟病菌致病过程中的关键作用，并提出靶向其酶活的抑制剂作为潜在的植物保护手段，拓展了结构生物学在病害防控中的应用前景。

Abstract:

Plant pathogenic fungi release cell wall-degrading enzymes (CWDEs), which are significant weapons for breaking down plant cell walls, although only a few reports focus on their pathogenesis. The current study demonstrates that MoFco1, a conserved α-L-fucosidase in several pathogenic fungi, degrades the hemicellulose component XXFG and contributes to the pathogenicity of Magnaporthe oryzae. In addition, MoFco1 enzyme activity is essential for its pathogenic function, as the enzyme activity mutation induced pathogenesis defects identical to the ΔMofco1 mutant. We further performed a structure-based virtual screening targeting MoFco1 and discovered 0989, which binds to MoFco1 and effectively inhibits M. oryzae pathogenesis. In brief, our study reveals the pathogenic mechanism of MoFco1 and explored the application of structure-based virtual screening in plant protection.

Key words: Magnaporthe oryzae , α-L-fucosidase , cell wall-degrading enzyme , virtual screening

Chang’an Ji, Zhao Hu, Yifang Zhang, Xia Song, Lei Su, Jintao Wang, Linxun Wu, Muxing Liu, Gang Li, Haifeng Zhang, Leiyun Yang, Xinyu Liu, Zhengguang Zhang. 岩藻糖苷酶MoFco1调控稻瘟病菌侵染的机制研究[J]. Journal of Integrative Agriculture, 2026, 25(9): 3736-3745.

Chang’an Ji, Zhao Hu, Yifang Zhang, Xia Song, Lei Su, Jintao Wang, Linxun Wu, Muxing Liu, Gang Li, Haifeng Zhang, Leiyun Yang, Xinyu Liu, Zhengguang Zhang. A novel fucosylation-specific cell wall-degrading enzyme promotes Magnaporthe oryzae infection[J]. Journal of Integrative Agriculture, 2026, 25(9): 3736-3745.

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