Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3508-3510.DOI: 10.1016/j.jia.2025.12.042

• • 上一篇    

冠状病毒粒子包裹病毒负链基因组

  

  • 收稿日期:2025-09-28 修回日期:2025-12-22 接受日期:2025-11-25 出版日期:2026-08-20 发布日期:2026-07-09

Coronavirus-like particles package the negative-strand genome of coronavirus

Xinyu Yang*, Wenzhen Qin*, Ning Kong*, Yuchang Liu, Guangzhi Tong, Tongling Shan#   

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China

  • Received:2025-09-28 Revised:2025-12-22 Accepted:2025-11-25 Online:2026-08-20 Published:2026-07-09
  • About author:#Correspondence Tongling Shan, E-mail: shantongling@shvri.ac.cn * These authors contributed equally to this study.
  • Supported by:

    This work was supported by the National Key Research and Development Programs of China (2023YFD1801300) and the National Natural Science Foundation of China (32272999).

摘要:

冠状病毒是单链正义 RNA 病毒。在 PEDV 吸附和内化后及病毒负链 RNA 合成前,我们同时检测到了冠状病毒的正链和负链序列。为解释上述发现,本研究证实α、β和δ冠状病毒属的冠状病毒病毒粒子中包裹病毒的负链基因组。通过蔗糖密度梯度离心纯化,并经抗体和核酸酶处理的 PEDV 颗粒中,约 20% 至 46% 含有负链基因组,且这些病毒颗粒具备吸附和内化的能力。该研究解释了冠状病毒在吸附和内化后检测到病毒的负链RNA的发现,也表明了冠状病毒在组装过程中不仅包装病毒正链基因组,也会同时包装了病毒负链基因组。