油菜素甾醇介导轻度土壤落干缓解高温胁迫导致的光温敏核不育系水稻雌蕊受精障碍

doi:10.1016/j.jia.2025.04.036

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (7): 2755-2770.DOI: 10.1016/j.jia.2025.04.036

油菜素甾醇介导轻度土壤落干缓解高温胁迫导致的光温敏核不育系水稻雌蕊受精障碍

收稿日期:2025-01-12 修回日期:2025-04-25 接受日期:2025-04-10 出版日期:2026-07-20 发布日期:2026-06-09

Brassinosteroids facilitate controlled soil drying to mitigate heat stress on pistil fertilization in photo-thermosensitive genetic male-sterile rice

Weiyang Zhang^{1, 2#}, Wei Cai^{1, 2}, Yujiao Zhou^{1, 2}, Ying Liu^{1, 2}, Wenqian Miao^{1, 2}, Kuanyu Zhu^{1, 2}, Weilu Wang³, Yunji Xu³, Yidi Sun⁴, Junfei Gu^{1, 2}, Hao Zhang^{1, 2}, Zhiqin Wang^{1, 2}, Lijun Liu^{1, 2}, Jianhua Zhang^{5, 6}, Jianchang Yang^{1, 2#}

¹Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College, Yangzhou University, Yangzhou 225009, China
²Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
³Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
⁴College of Hydraulic Science and Engineering, Yangzhou University, Yangzhou 225009, China
⁵Department of Biology, Hong Kong Baptist University, Hong Kong 999077, China
⁶State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong 999077, China

Received:2025-01-12 Revised:2025-04-25 Accepted:2025-04-10 Online:2026-07-20 Published:2026-06-09
About author:#Correspondence Weiyang Zhang, E-mail: wyz@yzu.edu.cn; Jianchang Yang, E-mail: jcyang@yzu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (32372214, 31901445, 32272198, 32071943, 31771710, and 32201888), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD-2020-01), the Top Talent Supporting Program of Yangzhou University, China (YZU-2028-01), and the Hong Kong Research Grants Council, China (GRF 12101722, 12102423, and 12105824).

摘要/Abstract

摘要：

全球范围内频繁发生的极端高温（HT）事件严重制约了水稻生产。本研究旨在探究轻度土壤落干（MD）这一可控措施能否替代传统的充分灌溉（WW）模式，从而更有效地缓解高温胁迫对光温敏核不育（PTGMS）水稻雌蕊受精的影响，并研究油菜素甾醇（BRs）在此过程中所起的作用。本研究选取两个PTGMS水稻品种，在开花期分别置于正常温度（NT）和HT处理，同时设置WW和MD两种灌溉模式。在传统的WW模式下，淹水会使根系和雌蕊中的BRs因过度分解而含量降低，削弱了由根系活力驱动的主动吸水过程，也无法缓解因叶片气孔开放受限而受阻的蒸腾拉力所驱动的被动吸水过程。这导致植株体内水分失衡，抗坏血酸-谷胱甘肽（AsA - GSH）循环受到抑制，烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NOX）活性过强，进而削弱了雌蕊功能。在HT胁迫下，这进一步加剧了雌蕊受精障碍，导致杂交种子产量下降。相反，采用MD模式可促进根系和雌蕊中BRs的合成并抑制其分解，从而增强根系活力和蒸腾驱动的吸水能力，维持了植株的水分平衡。同时，MD模式通过抑制雌蕊中NOX的活性，并增强AsA - GSH驱动的氧化还原内稳态，保障了雌蕊功能，进而有效缓解了HT胁迫引起的雌蕊受精障碍和杂交种子产量损失。本研究还通过遗传学和化学方法进一步证实了BRs在介导MD缓解HT胁迫下PTGMS水稻雌蕊受精障碍中的确切功能。综上所述，与传统的WW模式相比，MD模式通过促进BRs含量增加能更有效地缓解HT胁迫对PTGMS水稻雌蕊受精的负面影响。

Abstract:

Globally recurrent extreme high temperature (HT) events severely limit rice production. This study investigated whether a controlled moderate soil drying (MD) could replace the conventional well-watered (WW) regime to more effectively mitigate HT stress on pistil fertilization in photo-thermosensitive genetic male-sterile (PTGMS) rice, and examined the role of brassinosteroids (BRs). Two PTGMS rice varieties were cultivated under normal temperature (NT) and HT conditions, paired with WW and MD strategies during anthesis. In the conventional WW regime, waterlogging reduced BR levels in roots and pistils due to excessive decomposition, weakening active water uptake driven by root activity and failing to alleviate transpiration-pulled passive water extraction hampered by restricted stomatal openings. Thereby, it caused water imbalance in plants and weakened pistil function due to a suppressed ascorbate-glutathione (AsA-GSH) cycle and hyperactive nicotinamide adenine dinucleotide phosphate oxidase (NOX) activity. This exacerbated pistil fertilization impairment and hybrid seed yield loss under HT stress. Conversely, by promoting BR synthesis and inhibiting its decomposition in roots and pistils, the MD strategy enhanced root activity and transpiration-driven water uptake. It maintained plant water balance and supported pistil function by suppressing NOX activity and enhancing AsA-GSH cycle-driven redox homeostasis. Thus, it mitigated HT-induced pistil fertilization impairment and hybrid seed yield loss. The precise function of BRs in moderating the protective effects of MD against the detrimental impacts of HT stress on pistil fertilization in PTGMS rice was confirmed through genetic and chemical approaches. Consequently, a controlled MD method proved more effective than the conventional WW regime in alleviating HT stress on pistil fertilization in PTGMS rice by promoting BR enhancement.

Key words: high temperature , moderate soil drying , brassinosteroids , photo-thermosensitive genetic male-sterile (PTGMS) rice , pistil fertilization

Weiyang Zhang, Wei Cai, Yujiao Zhou, Ying Liu, Wenqian Miao, Kuanyu Zhu, Weilu Wang, Yunji Xu, Yidi Sun, Junfei Gu, Hao Zhang, Zhiqin Wang, Lijun Liu, Jianhua Zhang, Jianchang Yang. 油菜素甾醇介导轻度土壤落干缓解高温胁迫导致的光温敏核不育系水稻雌蕊受精障碍[J]. Journal of Integrative Agriculture, 2026, 25(7): 2755-2770.

Weiyang Zhang, Wei Cai, Yujiao Zhou, Ying Liu, Wenqian Miao, Kuanyu Zhu, Weilu Wang, Yunji Xu, Yidi Sun, Junfei Gu, Hao Zhang, Zhiqin Wang, Lijun Liu, Jianhua Zhang, Jianchang Yang. Brassinosteroids facilitate controlled soil drying to mitigate heat stress on pistil fertilization in photo-thermosensitive genetic male-sterile rice[J]. Journal of Integrative Agriculture, 2026, 25(7): 2755-2770.

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油菜素甾醇介导轻度土壤落干缓解高温胁迫导致的光温敏核不育系水稻雌蕊受精障碍

Brassinosteroids facilitate controlled soil drying to mitigate heat stress on pistil fertilization in photo-thermosensitive genetic male-sterile rice

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