寄生中国倒挂金钟的草莓滑刃线虫鉴定

doi:10.1016/j.jia.2024.07.002

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (2): 769-774.DOI: 10.1016/j.jia.2024.07.002

寄生中国倒挂金钟的草莓滑刃线虫鉴定

收稿日期:2024-01-18 修回日期:2024-07-08 接受日期:2024-04-29 出版日期:2026-02-20 发布日期:2026-01-06

Integrative identification of Aphelenchoides fragariae (Nematoda: Aphelenchoididae) parasitizing Fuchsia hybrid in China

Qiuling Huang^{1, 2}, Yan Liao¹, Chunhui Huang¹, Huan Peng³, Lingchiu Tsang¹, Borong Lin^{1, 2}, Deliang Peng³, Jinling Liao¹, Kan Zhuo^{1, 2#}

¹College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
²Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China
³State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2024-01-18 Revised:2024-07-08 Accepted:2024-04-29 Online:2026-02-20 Published:2026-01-06
About author:Qiuling Huang, E-mail: 1026992738@qq.com; #Correspondence Kan Zhuo, E-mail: zhuokan@scau.edu.cn
Supported by:
This research was financially supported by the Shaanxi Innovation Team Project (2024RS-CXTD-73) and the National Natural Science Foundation of China (31772136).

摘要/Abstract

摘要：

【目的】四川省成都市倒挂金钟（Fuchsia × hybrid Voss.）发生严重的叶斑病，从病叶中分离到大量滑刃线虫属线虫，对其形态学、分子特征和寄生性等进行详细研究，以明确其种类及对倒挂金钟等作物的寄生性，为草莓滑刃线虫的准确鉴定及倒挂金钟叶斑病防控提供科学依据。【方法】将倒挂金钟的发病叶片剪碎，用浸泡法分离叶片中的滑刃线虫，通过胡萝卜愈伤组织对该线虫进行纯化培养，分离纯化培养的线虫制作永久玻片，在光学显微镜和扫描电子显微镜下观察雌虫和雄虫的形态特征，并进行形态特征测量。采用两对引物1096F/1912R及1813F/2646R分别扩增18S小亚基部分区段（SSU）、引物D2A和D3B扩增28S大亚基D2-D3区段（LSU D2D3）及引物18S和26S扩增内转录间隔区（internal transcribed spacer, ITS），经序列测定，采用贝叶斯法构建该线虫种群与滑刃线虫属其他线虫种群的SSU、LSU D2D3和ITS系统进化树，分析其系统发育关系。在室内采用人工接种的方法，测定该线虫对倒挂金钟、草莓和蜈蚣凤尾蕨的寄生性。【结果】形态学观察和特征测量结果表明，从四川成都倒挂金钟病叶中分离到的滑刃线虫雌虫和雄虫形态特征与草莓滑刃线虫（Aphelenchoides fragariae）的形态特征基本一致；序列比对发现该线虫的SSU、LSU D2D3和ITS均与草莓滑刃线虫的序列相似性最高，在3个rDNA系统发育树中，该线虫也均与草莓滑刃线虫以100%的支持率形成一个单系。结合形态学和分子生物学结果，将分离自四川成都倒挂金钟病叶上的滑刃线虫鉴定为草莓滑刃线虫。寄生性研究结果表明，该线虫不仅可寄生和危害倒挂金钟，也可寄生和危害草莓和蜈蚣凤尾蕨这两种草莓滑刃线虫的典型寄主。【结论】引起我国四川成都倒挂金钟叶斑病的病原为草莓滑刃线虫，系首次发现草莓滑刃线虫危害倒挂金钟，也系国内首次通过结合形态学和分子特征对草莓滑刃线虫进行确定。

Abstract:

The strawberry crimp nematode (Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions, including China. A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants (Fuchsia×hybrida Hort. ex Sieb. & Voss.) in Chengdu, Sichuan Province, China. Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy. Detailed examination revealed diagnostic features consistent with A. fragariae. Three ribosomal DNA (rDNA) regions, i.e., partial small subunit (SSU) rRNA, D2-D3 expansion segments of the large subunit (LSU) rRNA, and the internal transcribed spacer (ITS), were amplified and sequenced. Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A. fragariae specimens, clearly separated from other Aphelenchoides species. Furthermore, host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida, but also on Fragaria ananassa and Pteris vittata, two known hosts of A. fragariae. Collectively, morphological, molecular, and host-range evidence confirm the identification of this nematode as A. fragariae. To our knowledge, this represents the first molecular and morphological confirmation of A. fragariae in China, and the first report of Fuchsia×hybrida as a natural host for this species.

Key words: strawberry crimp nematode , morphology , molecular characterization , host-suitability test , fuchsia

Qiuling Huang, Yan Liao, Chunhui Huang, Huan Peng, Lingchiu Tsang, Borong Lin, Deliang Peng, Jinling Liao, Kan Zhuo. 寄生中国倒挂金钟的草莓滑刃线虫鉴定[J]. Journal of Integrative Agriculture, 2026, 25(2): 769-774.

Qiuling Huang, Yan Liao, Chunhui Huang, Huan Peng, Lingchiu Tsang, Borong Lin, Deliang Peng, Jinling Liao, Kan Zhuo. Integrative identification of Aphelenchoides fragariae (Nematoda: Aphelenchoididae) parasitizing Fuchsia hybrid in China[J]. Journal of Integrative Agriculture, 2026, 25(2): 769-774.

参考文献

CABI (Commonwealth Agricultural Bureaux International). 2022. Aphelenchoides fragariae (strawberry crimp nematode). [2023-12-25]. https://www.cabidigitallibrary.org/doi/10.1079/cabicompendium.6381

Cai J J, Gu J F, Wang X, Fang Y W, Li H M. 2020. Aphelenchoides smolae n. sp. (Tylenchina: Aphelenchoididae) found in Lilium orientalis imported into China from the Netherlands. Nematology, 22, 799–813.

Carta L K, Li S, Skantar A M, Newcombe G. 2016. Morphological and molecular characterization of two Aphelenchoides endophytic in poplar leaves. Journal of Nematology, 48, 28–33.

Castillo P, Trapero-Casas J L, Jiménez-Díaz R M. 1995. Effect of time, temperature, and inoculum density on reproduction of Pratylenchus thornei in carrot disk cultures. Journal of Nematology, 27, 120–124.

Christie J R. 1932. Recent observations on the strawberry dwarf nematode in Massachusetts. Plant Disease Reporter, 16, 113–114.

EPPO (European and Mediterranean Plant Protection Organization) Global Database. 2023. Aphelenchoides fragariae. [2023-12-25]. https://gd.eppo.int/taxon/APLOFR/distribution

Fang Y S, Yin G L. 1999. Identify and studies on biological diversity of nematodes in forest tree in Guangdong province. Journal of Yunnan Agricultural University, 14, 114–115. (in Chinese)

Feng Z X. 2001. Plant Nematology. Chinese Agricultural Publishing, Beijing. pp. 172–184. (in Chinese)

Goodey J B, Franklin M T, Hooper D J. 1965. T. Goodey’s: The Nematode Parasites of Plants Catalogued Under Their Hosts, 3rd ed. Commonwealth Agricultural Bureaux, Farnham Royal, Bucks, England. p. 214.

Holterman M, van der Wurff A, van den Elsen S, van Megen H, Bongers T, Holovachov O, Bakker J, Helder J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution, 23, 1792–1800.

Hooper D J, Clark S A. 1980. Scanning electron micrographs of the head region of some species of Aphelenchoidea (Aphelenchina: Nematoda). Nematologica, 26, 47–56.

Huang R E, Ye W M, Liang J, Lu Q, Zhang X Y. 2012. Tylaphelenchus jiaae n. sp. and Aphelenchoides varicaudatus (Nematoda: Aphelenchoidinae) from Simao pine in Yunnan Province, China. Nematology, 14, 93–108.

Hunt D J. 1993. Aphelenchida, Longidoridae and Trichodoridae: Their Systematics and Bionomics. Commonwealth Agricultural Bureaux International, Wallingford. pp. 1–63.

Hunt D J. 2008. A checklist of the Aphelenchoidea (Nematoda: Tylenchina). Journal of Nematode Morphology and Systematics, 10, 99–135.

Jagdale G B, Grewal P S. 2002. Identification of alternatives for the management of foliar nematodes in floriculture. Pest Management Science, 58, 451–458.

Jian J Z, Huang W K, Kong L A, Jian H, Abdulsalam S, Peng D L, Peng H. 2023. Molecular diagnosis and direct quantification of cereal cyst nematode (Heterodera filipjevi) from field soil using TaqMan real time PCR. Journal of Integrative Agriculture, 22, 2591–2601.

Khan Z, Son S H, Moon H S, Kim S G, Shin H D, Jeon Y H, Kim Y H. 2007. Description of a foliar nematode, Aphelenchoides fragariae (Nematoda: Aphelenchida) with additional characteristics from Korea. Journal of Asia-Pacific Entomology, 10, 313–315.

Lehman P S. 2004. Cost-benefits of nematode management through regulatory programs. In: Chen Z X, Chen S Y, Dickson D W, eds., Nematology Advances and Perspectives. vol. 2. Commonwealth Agricultural Bureaux International, Willingford. pp. 1133–1177.

Li D Z. 1984. Descriptions of some species parasiting nematodes of genus Aphelenchoides plant above-ground part in Sichuan province. Journal of Southwest Agricultural College, 28, 70–74. (in Chinese)

Mobasseri M, Pourjam E, Pedram M. 2018. Morphological and molecular characterisation of Aphelenchoides primadentus n. sp. (Nematoda: Aphelenchoididae) from northern Iran. Nematology, 20, 97–109.

Mundo-Ocampo M, Troccoli A, Subbotin S A, Cid del Prado I, Baldwin J G, Inserra R N. 2008. Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb. n. and H. orientalis comb. n. (Tylenchida: Heteroderidae). Nematology, 10, 611–632.

Sánchez-Monge A, Flores L, Salazar L, Hockland S, Bert W. 2015. An updated list of the plants associated with plant-parasitic Aphelenchoides (Nematoda: Aphelenchoididae) and its implications for plant-parasitism within this genus. Zootaxa, 4013, 207–224.

Santos E C, Da Silva I L, Rosa R O, oliveira C M G, Buonicontro D S. 2021. Pathogenicity of Aphelenchoides pseudogoodeyi on the ornamental plants Asplenium nidus and Lilium speciosum in Brazil. Helminthologia, 58, 74–84.

Shahina F. 1996. A diagnostic compendium of the genus Aphelenchoides Fischer, 1894 (Nematoda: Aphelenchida) with some new records of the group from Pakistan. Pakistan Journal of Nematology, 14, 1–32.

Siddiqi M R. 1975. Aphelenchoides fragariae. C.I.H. Descriptions of Plant-parasitic Nematodes. Set 5, No. 74. Commonwealth Agricultural Bureaux International, Wallingford, UK. p. 4.

Sturhan D. 1962. Fber neue Wirtspflanzen der BlattSlchen Aphelenchoides fragariae und A. ritzemabosi, mit Bemerkungen zu den Wirtspflanzenkreisen beider Nematodenarten. Anzeiger für SchSdlingskunde, 35, 65–67. (in German)

Tanha Maafi Z, Subbotin S A, Moens M. 2003. Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on the ITS sequences of rDNA. Nematology, 5, 99–111.

Wang X, Wang P, Gu J F, Wang J L, Li H M. 2013. Description of Aphelenchoides xui sp. n. (Nematoda: Aphelenchoididae) in packaging wood from South Africa. Nematology, 15, 279–289.

Xie Z C, Chen L F, Liu G K, Zhang S S. 2004. Identification of plant nematodes species on strawberry in Fujian, China. Journal of Laiyang Agricultural College, 21, 99–103. (in Chinese)

Xu J H, Cheng H R, Fang Z D. 1989. Studies on a dwarf disease of strawberry in Jiangsu. Acta Phytopathologica Sinica, 19, 11–16. (in Chinese)

Yin Y Q. 1995. Surveys of parasitic nematodes on Mango in Guangdong, China. Acta Phytopathologica Sinica, 25, 42. (in Chinese)

Zhang X J, Zhang Y C, Cheng H R, Fang Z D. 1988. Studies on the potted chulan tree leaf-spot disease caused by a nematode I. Identification of the causal organism. Journal of Nanjing Agricultural University, 11, 41–46. (in Chinese)

Zhang Y C, Zhang X J, Cheng H R, Fang Z D. 1989. Studies on the occurrence and development of potted chulan tree leaf-spot disease caused by Aphelenchoides fragariae. Acta Agriculturae Shanghai, 5, 69–74. (in Chinese)

Zhao Z Q, Davies K A, Riley I T, Nobbs J M. 2007. Laimaphelenchus heidelbergi sp. nov. (Nematoda: Aphelenchina) from Victoria, Australia, and emendment of the diagnosis of the genus. Transactions of the Royal Society of South Australia, 131, 182–191.

Zhen F, Agudelo P. 2012. A protocol for assessing resistance to Aphelenchoides fragariae in Hosta cultivars. Plant Disease, 96, 1438–1444.

Zhu J H, Fan M Z, Liu J F, Tian F. 1997. Detection of a spring dwarf disease of strawberry in Hebei. Journal of Agricultural University of Hebei, 20, 21–23. (in Chinese)

Zhuo K, Cui R Q, Ye W M, Luo M, Wang H H, Hu X N, Liao J L. 2010. Morphological and molecular characterization of Aphelenchoides fujianensis n. sp. (Nematoda: Aphelenchoididae) from Pinus massoniana in China. Zootaxa, 2509, 39–52.

寄生中国倒挂金钟的草莓滑刃线虫鉴定

Integrative identification of Aphelenchoides fragariae (Nematoda: Aphelenchoididae) parasitizing Fuchsia hybrid in China

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics