氮吸收和碳氮协同转运提高dep1近等基因系水稻产量和氮利用效率

doi:10.1016/j.jia.2025.02.050

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (12): 4787-4809.DOI: 10.1016/j.jia.2025.02.050

氮吸收和碳氮协同转运提高dep1近等基因系水稻产量和氮利用效率

收稿日期:2024-11-28 修回日期:2025-02-24 接受日期:2025-02-08 出版日期:2025-12-20 发布日期:2025-11-13

Nitrogen uptake and carbon–nitrogen synergistic translocation improve yield and nitrogen use efficiency in the dep1 rice line

Guohui Li^{1, 2, 3}, Yan Zhang^{1, 2, 3}, Jiwei Xu^{1, 2, 3}, Changjin Zhu^{1, 2, 3}, Qiuqian Hu⁴, Ke Xu^{1, 2, 3#}

¹Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College, Yangzhou University, Yangzhou 225009, China
²Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
³Research Institute of Rice Industrial Engineering Technology, Yangzhou University, Yangzhou 225009, China
⁴School of Landscape and Horticulture, Yangzhou Polytechnic University, Yangzhou 225009, China

Received:2024-11-28 Revised:2025-02-24 Accepted:2025-02-08 Online:2025-12-20 Published:2025-11-13
About author:Guohui Li, Mobile: +86-15623758326, E-mail: lgh@yzu.edu.cn; #Correspondence Ke Xu, Tel: +86-514-87972435, E-mail: xuke@yzu.edu.cn
Supported by:
This research was funded by the National Natural Science Foundation of China (32272200 and 31901425), the Jiangsu Provincial Key Research and Development Program, China (BE2021361), the Jiangsu Provincial Carbon Peak and Carbon Neutrality Technology Innovation Special Fund Project, China (BE2022425), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), and the Lv Yang Jin Feng Talent Plan of Yangzhou City, China (YZLYJFJH2022YXBS020). We are grateful to Dr. Fei Wang (Huazhong Agricultural University, China) for providing the rice seeds.

摘要/Abstract

摘要：

提高水稻产量和氮利用效率是协调粮食生产和环境健康的关键挑战，然而水稻高产与氮高效协同的生理机制尚不明确。本研究以两个近等基因系水稻（DEP1和dep1）为材料，在三个氮水平（0、120和270 kg ha^-1）下进行了为期两年的田间试验，探究其碳氮转运的农艺和生理特征。结果表明，dep1相较于DEP1具有更高的产量、结实率、氮吸收和氮利用效率。灌浆期dep1茎中非结构性碳水化合物（NSCs）和氮向籽粒的转运显著高于DEP1，并且茎中NSCs转运与产量显著正相关，氮转运与氮利用效率也显著正相关。进一步分析发现，dep1的关键碳代谢酶活性（包括茎中α-淀粉酶、β-淀粉酶和蔗糖磷酸合成酶，以及籽粒中蔗糖合成酶、ADP-葡萄糖焦磷酸化酶和淀粉合成酶）显著高于DEP1，且其茎中蔗糖转运相关基因（OsSUT1和OsSWEET13）表达显著上调，这些特征促进了茎中NSCs的高效转运。同时，dep1茎中OsNPF2.4的高表达显著增强了氮的转运。对根系分析表明，dep1具有更优的根系形态特征（根干重、根表面积、根长和体积）和结构特征（中柱直径、皮层厚度和导管截面积），这解释了其较高的氮吸收能力，而且籽粒中OsNADH-GOGAT1和OsGS1.3的高表达促进了铵的同化，进一步增强了dep1的氮吸收效率。增加施氮量减少了碳的转运，但通过调节氮代谢酶活性和基因表达提高了氮的转运。总之，本研究结果强调了氮吸收以及茎中碳氮转运是dep1近等基因系水稻实现产量和氮利用效率协同提高的关键生理特征。

Abstract:

Improving rice yield and nitrogen use efficiency (NUE) are crucial challenges for coordinating food production and environmental health. However, little is known about the physiological mechanisms underlying the synergistic effects of high yield and NUE in rice. Using two near-isogenic rice lines (named DEP1 and dep1), a two-year field experiment was conducted to assess agronomic characteristics and the physiological characteristics of carbon and nitrogen translocation under three nitrogen levels. Compared with DEP1, dep1 had higher grain yield, grain filling percentage, nitrogen (N) uptake, and NUE. More non-structural carbohydrates (NSCs) and N in the stems were translocated to grains during grain filling in dep1 than in DEP1. Furthermore, stem NSC translocation was significantly positively correlated with grain yield, while stem N translocation was significantly positively correlated with NUE. Key carbon metabolism enzyme activities (α-amylase, β-amylase and sucrose-phosphate synthase in stems, and sucrose synthase, ADP-glucose pyrophosphorylase and starch synthase in grains) and stem sucrose transporter gene (OsSUT1 and OsSWEET13) expression were higher in dep1 than in DEP1. This contributed to high stem NSC translocation. Higher N translocation in the stems occurred due to the higher expression of OsNPF2.4. Moreover, the higher values of root morphological traits (root dry weight, root surface area, root length and root volume) and structural characteristics (stele diameter, cortical thickness and vessel section area) in dep1 explained its high nitrogen uptake. In addition, higher expression of OsNADH-GOGAT1 and OsGS1.3 promoted the assimilation of ammonium and contributed to higher nitrogen uptake in dep1. The application of N reduced carbon translocation but enhanced N translocation by regulating the corresponding metabolic enzyme activities and gene expression. Overall, these findings highlighted the roles of nitrogen uptake, and carbon and nitrogen translocation from stems as crucial characteristics for synergistically improving yield and NUE in the dep1 rice line.

Key words: carbon and nitrogen translocation , root characteristics , enzyme activity , gene expression , rice (Oryza sativa L.)

Guohui Li, Yan Zhang, Jiwei Xu, Changjin Zhu, Qiuqian Hu, Ke Xu. 氮吸收和碳氮协同转运提高dep1近等基因系水稻产量和氮利用效率[J]. Journal of Integrative Agriculture, 2025, 24(12): 4787-4809.

Guohui Li, Yan Zhang, Jiwei Xu, Changjin Zhu, Qiuqian Hu, Ke Xu. Nitrogen uptake and carbon–nitrogen synergistic translocation improve yield and nitrogen use efficiency in the dep1 rice line[J]. Journal of Integrative Agriculture, 2025, 24(12): 4787-4809.

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氮吸收和碳氮协同转运提高dep1近等基因系水稻产量和氮利用效率

Nitrogen uptake and carbon–nitrogen synergistic translocation improve yield and nitrogen use efficiency in the dep1 rice line

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