揭秘铁皮石斛的抗旱性：从转录组分析和番茄抗旱性增强中获得新见解

doi:10.1016/j.jia.2025.06.001

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (11): 4282-4293.DOI: 10.1016/j.jia.2025.06.001

揭秘铁皮石斛的抗旱性：从转录组分析和番茄抗旱性增强中获得新见解

收稿日期:2024-07-22 修回日期:2025-06-02 接受日期:2024-12-05 出版日期:2025-11-20 发布日期:2025-10-13

Unlocking Dendrobium officinale’s drought resistance: Insights from transcriptomic analysis and enhanced drought tolerance in tomato

Lulu Yu^{1, 2}, Muhammad Ahsan Asghar³, Antonios Petridis³, Fei Xu^{1, 2#}

¹Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, China
²School of Life Sciences, Yangtze University, Jingzhou 434025, China
³Department of Food Science, Aarhus University, Aarhus 8210, Denmark

Received:2024-07-22 Revised:2025-06-02 Accepted:2024-12-05 Online:2025-11-20 Published:2025-10-13
About author:Lulu Yu, E-mail: lulu2019@whsw.edu.cn; #Correspondence Fei Xu, E-mail: feixu666@hotmail.com
Supported by:
This work was supported by the Hubei Provincial Natural Science Foundation, China (2022CFB009) and the National Natural Science Foundation of China (31900242).

摘要/Abstract

摘要：

铁皮石斛是一种兰科植物，耐干旱的能力强。铁皮石斛表现出的高耐旱性可以归因于其结构和成分特征，包括富含多糖和其他胶体物质的厚叶和茎。尽管如此，其有助于提高铁皮石斛耐旱性的分子机制还不清楚。在本研究中，我们让铁皮石斛缺水一到六个月，并进行生理和RNA测序分析，以确定它如何应对长期缺水以及哪些基因可以保护其抵御干旱。研究发现，缺水六个月后，铁皮石斛仍然具有活力，这可从它仅补水两天后就快速复苏中看出。对经过一个月缺水处理的铁皮石斛植株进行转录组分析发现，参与诸多进程的基因表达发生了变化，其中最突出的是应激反应、光合作用、植物激素信号传导、碳代谢和果糖/甘露糖途径等。在这些差异表达基因中，过氧化物酶4（POD4）和NAC37被显著上调，因此我们选择这两个基因来进一步研究在增强植物耐旱性中的作用。值得注意的是，过表达铁皮石斛POD4和NAC37基因的转基因番茄植株比对照植株更耐旱，表现为活力更强、结实率更高，并维持更高的呼吸速率和叶绿素水平，以及较低氧化损伤等。这些研究证明探索未充分利用的物种以挖掘抗旱相关基因具有重要价值，并确定了POD4和NAC37可作为提升植物抗旱性的潜在候选基因。

Abstract:

Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities. The remarkable drought tolerance of D. officinale stems from its structural and compositional features, including thick leaves and stems containing abundant polysaccharides and colloidal substances. Despite these adaptations, the underlying molecular mechanisms responsible for enhanced drought tolerance remain inadequately understood. This study subjected D. officinale to water restriction for periods ranging from 1 to 6 months, conducting physiological and RNA sequencing analyses to elucidate its long-term dehydration response mechanisms and identify drought-protective genes. Following 6 months of dehydration, D. officinale maintained viability, demonstrated by rapid growth resumption after merely 2 d of rehydration. Transcriptome analysis of D. officinale plants under 1-month dehydration revealed differential gene expression across various processes, predominantly in stress responses, photosynthesis, phytohormone signaling, carbon metabolism, and fructose/mannose pathways. Among these, PEROXIDASE4 (POD4) and NAC37 showed significant upregulation and were selected for further investigation of their roles in drought protection. Transgenic tomato plants overexpressing D. officinale’s POD4 and NAC37 genes exhibited superior drought tolerance compared to controls, displaying enhanced vigor, increased fruit production, higher respiration rates, elevated chlorophyll levels, and reduced oxidative damage. This research demonstrates the value of exploring underutilized species for drought-tolerance genes and identifies POD4 and NAC37 as promising candidates for improving drought tolerance through breeding programs.

Key words: Dendrobium officinale , drought stress , drought tolerance , tomato , transcriptomics

Lulu Yu, Muhammad Ahsan Asghar, Antonios Petridis, Fei Xu. 揭秘铁皮石斛的抗旱性：从转录组分析和番茄抗旱性增强中获得新见解[J]. Journal of Integrative Agriculture, 2025, 24(11): 4282-4293.

Lulu Yu, Muhammad Ahsan Asghar, Antonios Petridis, Fei Xu. Unlocking Dendrobium officinale’s drought resistance: Insights from transcriptomic analysis and enhanced drought tolerance in tomato[J]. Journal of Integrative Agriculture, 2025, 24(11): 4282-4293.

参考文献

Ahmad I Z. 2019. Chapter 13 - Role of sugars in abiotic stress signaling in plants. In: Khan M I R, Reddy P S, Ferrante A, Khan N A, eds. Plant Signaling Molecules. Woodhead Publishing, United Kingdom. pp. 207–217.

Alshareef N O, Wang J Y, Ali S, Al-Babili S, Tester M, Schmöckel S M. 2019. Overexpression of the NAC transcription factor JUNGBRUNNEN1 (JUB1) increases salinity tolerance in tomato. Plant Physiology and Biochemistry, 140, 113–121.

Aluko O O, Ninkuu V, Ziemah J, Jianpei Y, Taiwo E, Ninkuu S B, Sabuli N, Adetunde L A, Imoro A W M, Ozavize S F, Onyiro Q A, Dogee G, Adedire O M, Hunpatin O S, Opoku N. 2024. Genome-wide identification and expression analysis of EIN3/EIL gene family in rice (Oryza sativa). Plant Stress, 12, 100437.

Benjamini Y, Hochberg Y. 1995. Controlling the false discovery rate: A practical and powerful approach to multiple testing. Journal of the Royal Statistical Society: Series B (Methodological), 57, 289–300.

Chen H, Bullock D A, Alonso J M, Stepanova A N. 2021. To fight or to grow: The balancing role of ethylene in plant abiotic stress responses. Plants, 11, 33.

Cutler S R, Rodriguez P L, Finkelstein R R, Abrams S R. 2010. Abscisic acid: Emergence of a core signaling network. Annual Review of Plant Biology, 61, 651–679.

Guo F, Han A, Gao H, Liang J, Zhao K, Cao S, Wang H, Wei Y, Shao X, Xu F. 2022. Mannose alleviates yellowing process of broccoli florets by regulating chlorophyll catabolism and delaying programmed cell death. Scientia Horticulturae, 295, 110888.

Huang H, Jiao Y, Tong Y, Wang Y. 2023. Comparative analysis of drought-responsive biochemical and transcriptomic mechanisms in two Dendrobium officinale genotypes. Industrial Crops and Products, 199, 116766.

Huang W, Duan W, Chen Y. 2022. Unravelling lake water storage change in Central Asia: Rapid decrease in tail-end lakes and increasing risks to water supply. Journal of Hydrology, 614, 128546.

Jian W, Zheng Y, Yu T, Cao H, Chen Y, Cui Q, Xu C, Li Z. 2021. SlNAC6, a NAC transcription factor, is involved in drought stress response and reproductive process in tomato. Journal of Plant Physiology, 264, 153483.

Jyoti S D, Azim J B, Robin A H K. 2021. Genome-wide characterization and expression profiling of EIN3/EIL family genes in Zea mays. Plant Gene, 25, 100270.

Lai C H, Huo C Y, Xu J, Han Q B, Li L F. 2024. Critical review on the research of chemical structure, bioactivities, and mechanism of actions of Dendrobium officinale polysaccharide. International Journal of Biological Macromolecules, 263, 130315.

Li Q, Xu F, Chen Z, Teng Z, Sun K, Li X, Yu J, Zhang G, Liang Y, Huang X, Du L, Qian Y, Wang Y, Chu C, Tang J. 2021. Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation. Nature Plants, 7, 1108–1118.

Li X D, Zhuang K Y, Liu Z M, Yang D Y, Ma N N, Meng Q W. 2016. Overexpression of a novel NAC-type tomato transcription factor, SlNAM1, enhances the chilling stress tolerance of transgenic tobacco. Journal of Plant Physiology, 204, 54–65.

Liu C, Li J, Zhu P, Yu J, Hou J, Wang C, Long D, Yu M, Zhao A. 2019. Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression. PeerJ, 7.

Masri R, Kiss E. 2023. The role of NAC genes in response to biotic stresses in plants. Physiological and Molecular Plant Pathology, 126, 102034.

Puranik S, Sahu P P, Srivastava P S, Prasad M. 2012. NAC proteins: Regulation and role in stress tolerance. Trends in Plant Science, 17, 369–381.

Ramadoss B R, Gangola M P, Gurunathan S. 2020. Chapter 4-NAC transcription factor family in rice: Recent advancements in the development of stress-tolerant rice. In: Wani S H, ed., Transcription Factors for Abiotic Stress Tolerance in Plants. Academic Press, United States. pp. 47–61.

Saidi M N, Mergby D, Souibgui A, Yacoubi I. 2022. Overexpression of durum wheat NAC transcription factor TtNTL3A promotes early flowering and increases multiple stress tolerance in transgenic arabidopsis. Plant Physiology and Biochemistry, 192, 1–9.

Samanta S, Seth C S, Roychoudhury A. 2024. The molecular paradigm of reactive oxygen species (ROS) and reactive nitrogen species (RNS) with different phytohormone signaling pathways during drought stress in plants. Plant Physiology and Biochemistry, 206, 108259.

Sharma A, Gupta A, Ramakrishnan M, Ha C V, Zheng B, Bhardwaj M, Tran L S P. 2023. Roles of abscisic acid and auxin in plants during drought: A molecular point of view. Plant Physiology and Biochemistry, 204, 108129.

Sreenivasulu N, Harshavardhan V T, Govind G, Seiler C, Kohli A. 2012. Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress? Gene, 506, 265–273.

Srivastava R, Kobayashi Y, Koyama H, Sahoo L. 2022. Overexpression of cowpea NAC transcription factors promoted growth and stress tolerance by boosting photosynthetic activity in Arabidopsis. Plant Science, 319, 111251.

Thanmalagan R R, Jayaprakash A, Roy A, Arunachalam A, Lakshmi P T V. 2022. A review on applications of plant network biology to understand the drought stress response in economically important cereal crops. Plant Gene, 29, 100345.

Thirumalaikumar V P, Devkar V, Mehterov N, Ali S, Ozgur R, Turkan I, Mueller-Roeber B, Balazadeh S. 2018. NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato. Plant Biotechnology Journal, 16, 354–366.

Velikova V, Yordanov I, Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Science, 151, 59–66.

Vurukonda S S K P, Vardharajula S, Shrivastava M, Skz A. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184, 13–24.

Wu X, Yuan J, Luo A, Chen Y, Fan Y. 2016. Drought stress and re-watering increase secondary metabolites and enzyme activity in Dendrobium moniliforme. Industrial Crops and Products, 94, 385–393.

Xu F, Yuan S, Zhang D W, Lv X, Lin H H. 2012a. The role of alternative oxidase in tomato fruit ripening and its regulatory interaction with ethylene. Journal of Experimental Botany, 63, 5705–5716.

Xu F, Zhang D W, Zhu F, Tang H, Lv X, Cheng J, Xie H F, Lin H H. 2012b. A novel role for cyanide in the control of cucumber (Cucumis sativus L.) seedlings response to environmental stress. Plant Cell and Environment, 35, 1983–1997.

Yadav B, Jogawat A, Rahman M S, Narayan O P. 2021. Secondary metabolites in the drought stress tolerance of crop plants: A review. Gene Reports, 23, 101040.

Yu L L, Liu C J, Peng Y, He Z Q, Xu F. 2022. New insights into the role of cyanide in the promotion of seed germination in tomato. BMC Plant Biology, 22, 28.

Yu L L, Liu Y, Peng Y, Zhu F, Xu F. 2021. Overexpression of cyanoalanine synthase 1 improves germinability of tobacco seeds under salt stress conditions. Environmental and Experimental Botany, 182, 104332.

Yu L L, Liu Y, Xu F. 2019. Comparative transcriptome analysis reveals significant differences in the regulation of gene expression between hydrogen cyanide- and ethylene-treated Arabidopsis thaliana. BMC Plant Biology, 19, 92.

Yu L L, Liu Y, Zhu F, Geng X X, Yang Y, He Z Q, Xu F. 2020. The enhancement of salt stress tolerance by salicylic acid pretreatment in Arabidopsis thaliana. Biologia Plantarum, 64, 150–158.

Zhang H, Sun X, Dai M. 2022. Improving crop drought resistance with plant growth regulators and rhizobacteria: Mechanisms, applications, and perspectives. Plant Communications, 3, 100228.

Zhang H, Zhao Y, Zhu J K. 2020. Thriving under stress: How plants balance growth and the stress response. Developmental Cell, 55, 529–543.

Zhao S Y, Zeng W H, Li Z, Peng Y. 2020. Mannose regulates water balance, leaf senescence, and genes related to stress tolerance in white clover under osmotic stress. Biologia Plantarum, 64, 406–416.

Zia R, Nawaz M S, Siddique M J, Hakim S, Imran A. 2021. Plant survival under drought stress: Implications, adaptive responses, and integrated rhizosphere management strategy for stress mitigation. Microbiological Research, 242, 126626.

揭秘铁皮石斛的抗旱性：从转录组分析和番茄抗旱性增强中获得新见解

Unlocking Dendrobium officinale’s drought resistance: Insights from transcriptomic analysis and enhanced drought tolerance in tomato

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics