Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (1): 398-402.DOI: 10.1016/j.jia.2024.11.010

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一种快速用于检测植物苯丙烷途径中黄酮类化合物的LC-QqQ-MS/MS方法开发

  

  • 收稿日期:2024-08-26 接受日期:2024-10-18 出版日期:2025-01-20 发布日期:2025-01-07

Development of a fast LC-QqQ-MS/MS method for detecting flavonoids in the phenylpropanoid pathway of plants

Dili Lai1, 2*, Yu Fan3*, Md. Nurul Huda1, Yuanfen Gao1, Tanzim Jahan1, Wei Li1, Yuqi He1, Kaixuan Zhang1, Jianping Cheng2, Jingjun Ruan2, Baoping Zhao4, Meiliang Zhou1#   

  1. 1 State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    2 College of Agriculture, Guizhou University, Guiyang 550025, China

    3 School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China

    4 Agronomy College, Inner Mongolia Agricultural University, Hohhot 010019, China

  • Received:2024-08-26 Accepted:2024-10-18 Online:2025-01-20 Published:2025-01-07
  • About author:#Correspondence Meiliang Zhou, E-mail: zhoumeiliang@caas.cn * These authors contributed equally to this study.
  • Supported by:

    This research was supported by the National Key Research and Development Program of China (2023YFD1600700 and 2023YFD1600701).  

摘要:

黄酮类化合物因其多种促进健康的作用而备受人们关注。然而,植物中存在许多结构相似的类黄酮,而且浓度往往很低,这在一定程度上增加了分离和鉴定的难度。液相色谱-三重四极杆飞行时间串联质谱法(LC-QqQ-MS/MS)成为检测类黄酮使用最广泛的技术之一。然而在植物中关于不同组织的黄酮类物质的系统检测方法较为匮乏。本研究系统构建了从苯丙烷开始到芦丁及其相关物质的检测方法,涉及黄酮化合物(21),香豆素3),花青素4),酚酸类4),原花青素4)和醌类2)。检测发现该方法适用于模式植物烟草和拟南芥部分物质的检测,高度适用于富含黄酮类物质的植物进行检测,如荞麦属植物。该方法的建立可以为了解苯丙烷途径黄酮类物质的动态变化提供助力,进而为深入理解基因功能提供支持。