人工合成六倍体小麦中主效粒长位点QGl.cau-2D.1的精细定位和多效性鉴定

doi:10.1016/j.jia.2023.09.009

摘要/Abstract

摘要：

籽粒大小是籽粒产量的决定因素之一，鉴定控制籽粒大小的遗传位点对产量的提升意义重大。在之前的研究中，利用面包小麦TAA10和人工合成六倍体小麦XX329衍生的F₂群体检测到一个与粒长(GL)有关的稳定QTL位点QGl.cau-2D.1。在本研究中，我们主要是为了对该位点精细定位和并对其遗传效应进行验证。以TAA10为轮回亲本，通过分子标记辅助选择获得多个近等基因系(NILs)。通过25个杂合单株衍生的次级分离群体对位点QGl.cau-2D.1进行精细定位，来自于XX329的增效基因使粒长、千粒重、总小穗数和穗密度增加，进一步利用2D+ (XX329)和2D−(TAA10)的NIL材料对该位点进行遗传和多效性的验证。根据中国春RefSeq v2.1的序列，将目标位点定位至约~0.9 Mb的区间。根据两亲本的重测序结果和表达谱差异分析将TraesCS2D03G0114900 (Os03g0594700)确定为候选基因。总之，QGl.cau-2D.1通过粒长的增加提高千粒重，并同时显著提升总小穗数。

Abstract:

Grain size is one of the determinants of grain yield, and identifying the genetic loci that control grain size will be helpful for increasing grain yield. In our previous study, a quantitative trait locus (QTL) for grain length (GL), QGl.cau-2D.1, was identified from an F2 population developed from the cross between the natural (TAA10) and synthetic (XX329) allohexaploid wheat. In the present study, we mainly fine mapped and validated its genetic effects. To this end, multiple near-isogenic lines (NILs) were obtained through marker-assisted selection with TAA10 as the recurrent parent. The secondary populations derived from 25 heterozygous recombinants were used for fine mapping of QGl.cau-2D.1, and the allele from XX329 significantly increased GL, thousand-grain weight (TGW), total spikelet number per spike (TSN) and spike compactness (SC). Using NILs for XX329 (2D+) and TAA10 (2D−), we determined the genetic and pleiotropic effects of QGl.cau-2D.1. The target sequences were aligned with the wheat reference genome RefSeq v2.1 and spanned an ~0.9 Mb genomic region. TraesCS2D03G0114900 (ortholog of Os03g0594700) was predicted as the candidate gene based on whole-genome re-sequencing and expression analyses. In summary, the map-based cloning of QGl.cau-2D.1 will be useful for improving grain weight with enhanced GL and TSN.

Key words: grain length , total spikelet number per spike , spike compactness , near-isogenic lines , QTL , wheat

Mingming Wang, Jia Geng, Zhe Zhang, Zihan Zhang, Lingfeng Miao, Tian Ma, Jiewen Xing, Baoyun Li, Qixin Sun, Yufeng Zhang, Zhongfu Ni. 人工合成六倍体小麦中主效粒长位点QGl.cau-2D.1的精细定位和多效性鉴定[J]. Journal of Integrative Agriculture, 2024, 23(9): 2911-2922.

Mingming Wang, Jia Geng, Zhe Zhang, Zihan Zhang, Lingfeng Miao, Tian Ma, Jiewen Xing, Baoyun Li, Qixin Sun, Yufeng Zhang, Zhongfu Ni. Fine mapping and characterization of a major QTL for grain length, QGl.cau-2D.1, that has pleiotropic effects in synthetic allohexaploid wheat[J]. Journal of Integrative Agriculture, 2024, 23(9): 2911-2922.

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