稻瘟病菌DNA损伤修复复合物MoMMS21-MoSMC5对病菌侵染相关的发育和致病性至关重要

doi:10.1016/j.jia.2024.01.010

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (6): 1956-1966.DOI: 10.1016/j.jia.2024.01.010

稻瘟病菌DNA损伤修复复合物MoMMS21-MoSMC5对病菌侵染相关的发育和致病性至关重要

收稿日期:2023-09-25 接受日期:2023-12-12 出版日期:2024-06-20 发布日期:2024-05-30

The DNA damage repair complex MoMMS21–MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

Yue Jiang^{1, 2}, Rong Wang³, Lili Du¹, Xueyu Wang³, Xi Zhang³, Pengfei Qi¹, Qianfei Wu¹, Baoyi Peng¹, Zonghua Wang^{1, 3, 4#}, Mo Wang^2#, Ya Li^{1, 3, 5#}

¹State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
²State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China
³Fujian Universities Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou 350002, China
⁴Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, China
⁵Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou 350003, China

Received:2023-09-25 Accepted:2023-12-12 Online:2024-06-20 Published:2024-05-30
About author:Yue Jiang, Tel: +86-591-83789365, E-mail: jiangyue8926@163.com; #Correspondence Zonghua Wang, Tel: +86-591-83789365, E-mail: zonghuaw@163.com; Mo Wang, Tel: +86-591-83789365, E-mail: wangmo108@163.com; Ya Li, Tel: +86-591-83789365, E-mail: liya-81@163.co
Supported by:
This research was supported by the National Key Research and Development Program of China (2023YFD1400200), the Natural Science Foundation of Fujian Province, China (2022J01125), the Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, China (MIMCP-202301), the Fujian Provincial Science and Technology Key Project, China (2022NZ030014), and the National Natural Science Foundation of China (NSFC31871914).

摘要/Abstract

摘要：

在酵母和动植物中，保守的DNA损伤修复复合物MMS21-SMC5/6已经被广泛研究。然而，在植物病原真菌中，尤其是在对水稻极具破坏性的稻瘟病菌（Magnaporthe oryzae）中，该复合物的功能尚不清楚。本文对MMS21-SMC5在稻瘟病菌中的同源物MoMMS21和MoSMC5进行了功能研究。首先，我们发现DNA损伤诱导剂（腐草霉素）能显著抑制稻瘟病菌营养生长、侵染相关发育（孢子产生、孢子萌发和附着胞形成）和致病性，表明DNA损伤修复对稻瘟病菌至关重要。其次，利用酵母双杂交、双分子荧光以及共定位实验发现，MoMMS21和MoSMC5在稻瘟病菌细胞核中互作，表明它们在稻瘟病菌中也作为复合物行使功能。最后，基因敲除实验表明，MoMMS21和MoSMC5对稻瘟病菌侵染相关发育和致病性都至关重要，而只有MoMMS21参与了病菌的营养生长和对腐草霉素的响应，MoSMC5的敲除对病菌的营养生长和腐草霉素敏感性没有影响。综上，该研究揭示了DNA损伤修复复合物MMS21-SMC5在稻瘟菌中的作用机制，突出了它们除DNA损伤修复之外的生物学功能差异。

Abstract: The conserved DNA damage repair complex, MMS21–SMC5/6 (Methyl methane sulfonate 21–Structural maintenance of chromosomes 5/6), has been extensively studied in yeast, animals, and plants. However, its role in phytopathogenic fungi, particularly in the highly destructive rice blast fungus Magnaporthe oryzae, remains unknown. In this study, we functionally characterized the homologues of this complex, MoMMS21 and MoSMC5, in M. oryzae. We first demonstrated the importance of DNA damage repair in M. oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth, infection-related development and pathogenicity in this fungus. Additionally, we discovered that MoMMS21 and MoSMC5 interacted in the nuclei, suggesting that they also function as a complex in M. oryzae. Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M. oryzae, while only MoMMS21 deletion affected growth and sensitivity to phleomycin, indicating its specific involvement in DNA damage repair. Overall, our results provide insights into the roles of MoMMS21 and MoSMC5 in M. oryzae, highlighting their functions beyond DNA damage repair.

Key words: Magnaporthe oryzae , MMS21 , SMC5 , DNA damage repair , pathogenicity

稻瘟病菌DNA损伤修复复合物MoMMS21-MoSMC5对病菌侵染相关的发育和致病性至关重要 [J]. Journal of Integrative Agriculture, 2024, 23(6): 1956-1966.

Yue Jiang, Rong Wang, Lili Du, Xueyu Wang, Xi Zhang, Pengfei Qi, Qianfei Wu, Baoyi Peng, Zonghua Wang, Mo Wang, Ya Li.

The DNA damage repair complex MoMMS21–MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae [J]. Journal of Integrative Agriculture, 2024, 23(6): 1956-1966.

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稻瘟病菌DNA损伤修复复合物MoMMS21-MoSMC5对病菌侵染相关的发育和致病性至关重要

The DNA damage repair complex MoMMS21–MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

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