独脚金内酯调控棉花纤维细胞伸长及次生壁增厚

doi:10.1016/j.jia.2023.07.009

摘要/Abstract

摘要： 棉花是全球重要的经济作物之一，也是纺织工业的主要纤维来源。独脚金内酯（SLs）是一类胡萝卜素衍生型植物激素，参与植物生长发育的许多过程，但是SLs在棉花纤维发育中的功能尚不清楚。本研究发现内源性SLs在开花后20天的棉花纤维组织中显著积累。添加外源性的SLs，可促进离体胚珠的纤维伸长和细胞壁增厚。本研究克隆了三个关键的SLs生物合成基因，即GhD27、GhMAX3和GhMAX4，RT-qPCR显示它们在纤维中高表达，表达蛋白定位于叶绿体。拟南芥中独脚金内酯合成缺陷突变体会出现多分枝的表型，将三个棉花的SLs生物合成基因转入相应的拟南芥独脚金内酯合成缺陷突变体，可以挽救突变体的多分枝表型。利用VIGS技术分别干涉棉花中GhD27、GhMAX3和GhMAX4的表达，干涉植株的腋芽和叶片数量增加，纤维长度变短，纤维细胞壁厚度显著降低。这些结果表明SLs参与棉花植株的生长、纤维的伸长和次生壁的形成。上述研究结果将为改良棉花纤维品质提供新的遗传资源。

Abstract:

Cotton is one of the most important economic crops in the world, and it is a major source of fiber in the textile industry. Strigolactones (SLs) are a class of carotenoid-derived plant hormones involved in many processes of plant growth and development, although the functions of SL in fiber development remain largely unknown. Here, we found that the endogenous SLs were significantly higher in fibers at 20 days post-anthesis (DPA). Exogenous SLs significantly increased fiber length and cell wall thickness. Furthermore, we cloned three key SL biosynthetic genes, namely GhD27, GhMAX3, and GhMAX4, which were highly expressed in fibers, and subcellular localization analyses revealed that GhD27, GhMAX3, and GhMAX4 were localized in the chloroplast. The exogenous expression of GhD27, GhMAX3, and GhMAX4 complemented the physiological phenotypes of d27, max3, and max4 mutations in Arabidopsis, respectively. Knockdown of GhD27, GhMAX3, and GhMAX4 in cotton resulted in increased numbers of axillary buds and leaves, reduced fiber length, and significantly reduced fiber thickness. These findings revealed that SLs participate in plant growth, fiber elongation, and secondary cell wall formation in cotton. These results provide new and effective genetic resources for improving cotton fiber yield and plant architecture.

Key words: strigolactones , fiber elongation , secondary cell wall thickening , cotton

. 独脚金内酯调控棉花纤维细胞伸长及次生壁增厚[J]. Journal of Integrative Agriculture, 2024, 23(6): 1850-1863.

Yunze Wen, Peng He, Xiaohan Bai, Huizhi Zhang, Yunfeng Zhang, Jianing Yu.

Strigolactones modulate cotton fiber elongation and secondary cell wall thickening [J]. Journal of Integrative Agriculture, 2024, 23(6): 1850-1863.

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