Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (6): 1825-1838.DOI: 10.1016/j.jia.2023.09.031

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Hippo信号通路的生物学价值和展望：对动物的骨骼肌等机体器官发育的影响

  

  • 收稿日期:2022-11-28 接受日期:2023-07-16 出版日期:2024-06-20 发布日期:2024-05-29

Biology of Hippo signaling pathway: Skeletal muscle development and beyond

Shuqi Qin1*, Chaocheng Li1*, Haiyan Lu2, Yulong Feng1, Tao Guo1, Yusong Han1, Yongsheng Zhang1#, Zhonglin Tang3, 4#   

  1. 1 Xinjiang Characteristic Livestock Breeding and Reproduction Team, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

    2 Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    3 Kunpeng Institute of Modern Agriculture at Foshan, Foshan 528226, China

    4 Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China

  • Received:2022-11-28 Accepted:2023-07-16 Online:2024-06-20 Published:2024-05-29
  • About author:Shuqi Qin, E-mail: qin_shu_qi@163.com; Chaocheng Li, E-mail: m18299374567@163.com; #Correspondence Yongsheng Zhang, E-mail: zhangyongsheng@shzu.edu.cn; Zhonglin Tang, E-mail: tangzhonglin@caas.cn * These authors contributed equally to this study.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31830090), the High-level Talent Project of Shihezi University, China (2022ZK022) and the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences (CAAS-ZDRW202006).

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摘要：

目前全球对农场动物及其肉类产品猪肉、鸡肉和其他畜禽肉类的需求正在稳步提升。随着生命科学研究的深入和生物技术的快速发展，开发先进的分子育种标记，高效地提升动物的产肉性状是一个很好的契机。Hippo是一个关键的信号通路，因其在器官大小调控中起着至关重要的作用。近年来，随着对Hippo信号通路研究的增加，通过基因组学、转录组学、蛋白质组学和代谢组学等多组学技术的联合应用，将有助于推进Hippo信号通路参与骨骼肌发育的深入研究。Hippo信号通路在诸多生物学事件中起着关键作用，主要包括：细胞分裂、细胞迁移、细胞增殖、细胞分化、细胞凋亡，以及细胞黏连、细胞极性和细胞面对机械过载中的稳态维持等。它在骨骼肌发育中的影响，对畜牧业的生产效益提升有重要的研究价值。在此，我们追溯了Hippo通路的起源，全面整理了该通路目前发现的所有功能性因子，深入解析了其发挥功能的分子机理，并从全新视角依据其主效结构域和作用模式进行归纳分类，系统探讨其在整个骨骼肌发育中的调控作用。尤其重点针对Hippo信号通路在胚胎干细胞发育、肌肉卫星细胞命运决定、肌肉发生、骨骼肌产肉量和器官大小调节、肌肉过度肥大和萎缩、肌纤维形成及其不同类型之间的转化，以及心肌细胞增殖和再生中的作用进行了有条理的总结和综合分析。本文对Hippo信号通路的总结和展望，为进一步高效率地提升产肉量和肌肉沉积，开发畜禽的分子育种新技术提供思路，将有助于动物分子育种的发展。

Abstract:

Global demand for farm animals and their meat products i.e., pork, chicken and other livestock meat, is steadily incresing. With the ongoing life science research and the rapid development of biotechnology, it is a great opportunity to develop advanced molecular breeding markers to efficiently improve animal meat production traits.  Hippo is an important study subject because of its crucial role in the regulation of organ size.  In recent years, with the increase of research on Hippo signaling pathway, the integrative application of multi-omics technologies such as genomics, transcriptomics, proteomics, and metabolomics can help promote the in-depth involvement of Hippo signaling pathway in skeletal muscle development research.  The Hippo signaling pathway plays a key role in many biological events, including cell division, cell migration, cell proliferation, cell differentiation, cell apoptosis, as well as cell adhesion, cell polarity, homeostasis, maintenance of the face of mechanical overload, etc.  Its influence on the development of skeletal muscle has important research value for enhancing the efficiency of animal husbandry production.  In this study, we traced the origin of the Hippo pathway, comprehensively sorted out all the functional factors found in the pathway, deeply analyzed the molecular mechanism of its function, and classified it from a novel perspective based on its main functional domain and mode of action.  Our aim is to systematically explore its regulatory role throughout skeletal muscle development.  We specifically focus on the Hippo signaling pathway in embryonic stem cell development, muscle satellite cell fate determination, myogenesis, skeletal muscle meat production and organ size regulation, muscle hypertrophy and atrophy, muscle fiber formation and its transformation between different types, and cardiomyocytes.  The roles in proliferation and regeneration are methodically summarized and analyzed comprehensively.  The summary and prospect of the Hippo signaling pathway within this article will provide ideas for further improving meat production and muscle deposition and developing new molecular breeding technologies for livestock and poultry, which will be helpful for the development of animal molecular breeding.

Key words: Hippo , skeletal muscle ,  organ size ,  myogenesis ,  C2C12 ,  livestock animals