甜樱桃SAUR基因家族分子特征及PavSAUR55在脱落过程中的功能分析

doi:10.1016/j.jia.2023.04.031

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (6): 1720-1739.DOI: 10.1016/j.jia.2023.04.031

甜樱桃SAUR基因家族分子特征及PavSAUR55在脱落过程中的功能分析

收稿日期:2022-09-16 修回日期:2023-04-28 接受日期:2023-03-24 出版日期:2023-06-20 发布日期:2023-03-24

Molecular characterization of the SAUR gene family in sweet cherry and functional analysis of PavSAUR55 in the process of abscission

HOU Qian-dong^1*, HONG Yi^1*, WEN Zhuang¹, SHANG Chun-qiong², LI Zheng-chun², CAI Xiao-wei¹, QIAO Guang¹, WEN Xiao-peng^1#

¹Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, P.R.China
²College of Forestry/Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang 550025, P.R.China

Received:2022-09-16 Revised:2023-04-28 Accepted:2023-03-24 Online:2023-06-20 Published:2023-03-24
About author:HOU Qian-dong, E-mail: qiandhou@163.com; HONG Yi, E-mail: hongyi715@163.com; #Correspondence WEN Xiao-peng, E-mail: xpwensc@hotmail.com *These authors contributed equally to this work.
Supported by:
This project was supported by grants from the National Natural Science Foundation of China (32272649) and the Core Program of Guizhou Education Department, China (KY 2021-038).

摘要/Abstract

摘要：

小生长素上调RNA（SAUR）是一个广泛分布于陆地植物中较大的一个基因家族。本研究对甜樱桃中的SAUR家族进行了综合分析，并利用遗传转化的方法鉴定了PavSAUR55的潜在生物学功能。甜樱桃基因组编码86个SAUR成员，其中大多数是无内含子的。进化上这些基因被分为七个亚类。基因复制事件表明甜樱桃基因组中发生了片段重复和串联重复事件。大多数成员在进化过程中主要经历纯化选择压力。在果实发育过程中，PavSAUR16/45/56/63的表达水平上调，PavSAUR12/61的表达水平下调。由于PavSAUR13/16/55/61在小果脱落过程中的显著差异表达，它们可能是参与调节甜樱桃生理性果实脱落的候选基因。PavSAUR55在拟南芥中的过表达产生了更早的生殖生长、根系伸长和延迟的花瓣脱落。此外，该基因并不会导致种子发芽时间的改变，并且能够在ABA处理下增加侧根的数量。这些结果表明，甜樱桃SAURs在小果脱落过程中起着重要的作用，并将有助于未来深入了解该果树中可能发生的严重小果脱落的机制奠定基础。

Abstract:

Small auxin up RNA (SAUR) is a large gene family that is widely distributed among land plants. In this study, a comprehensive analysis of the SAUR family was performed in sweet cherry, and the potential biological functions of PavSAUR55 were identified using the method of genetic transformation. The sweet cherry genome encodes 86 SAUR members, the majority of which are intron-less. These genes appear to be divided into seven subfamilies through evolution. Gene duplication events indicate that fragment duplication and tandem duplication events occurred in the sweet cherry. Most of the members mainly underwent purification selection pressure during evolution. During fruit development, the expression levels of PavSAUR16/45/56/63 were up-regulated, and conversely, those of PavSAUR12/61 were down-regulated. Due to the significantly differential expressions of PavSAUR13/16/55/61 during the fruitlet abscission process, they might be the candidate genes involved in the regulation of physiological fruit abscission in sweet cherry. Overexpression of PavSAUR55 in Arabidopsis produced earlier reproductive growth, root elongation, and delayed petal abscission. In addition, this gene did not cause any change in the germination time of seeds and was able to increase the number of lateral roots under abscisic acid (ABA) treatment. The identified SAURs of sweet cherry play a crucial role in fruitlet abscission and will facilitate future insights into the mechanism underlying the heavy fruitlet abscission that can occur in this fruit crop.

Key words: sweet cherry , small auxin up RNA , gene family , expression profile , fruitlet abscission

HOU Qian-dong, HONG Yi, WEN Zhuang, SHANG Chun-qiong, LI Zheng-chun, CAI Xiao-wei, QIAO Guang, WEN Xiao-peng. 甜樱桃SAUR基因家族分子特征及PavSAUR55在脱落过程中的功能分析[J]. Journal of Integrative Agriculture, 2023, 22(6): 1720-1739.

HOU Qian-dong, HONG Yi, WEN Zhuang, SHANG Chun-qiong, LI Zheng-chun, CAI Xiao-wei, QIAO Guang, WEN Xiao-peng. Molecular characterization of the SAUR gene family in sweet cherry and functional analysis of PavSAUR55 in the process of abscission[J]. Journal of Integrative Agriculture, 2023, 22(6): 1720-1739.

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甜樱桃SAUR基因家族分子特征及PavSAUR55在脱落过程中的功能分析

Molecular characterization of the SAUR gene family in sweet cherry and functional analysis of PavSAUR55 in the process of abscission

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