Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (10): 3103-3114.DOI: 10.1016/j.jia.2023.04.032

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Cd穿过美国白蛾蛹降低了周氏啮小蜂的寄生适合度：一个影响生物防治效率的潜在风险

  

  • 收稿日期:2022-11-08 接受日期:2023-02-20 出版日期:2023-10-20 发布日期:2023-10-07

Cadmium (Cd) exposure through Hyphantria cunea pupae reduces the parasitic fitness of Chouioia cunea: A potential risk to its biocontrol efficiency

YAN Shan-chun1, 2, WU Hong-fei1, 2, ZHENG Lin1, 2, TAN Ming-tao1, 2, JIANG Dun1, 2#   

  1. 1 School of Forestry, Northeast Forestry University, Harbin 150040, P.R.China
    2 Key Laboratory of Sustainable Forest Ecosystem Management, Ministry of Education, Northeast Forestry University, Harbin 150040, P.R.China
  • Received:2022-11-08 Accepted:2023-02-20 Online:2023-10-20 Published:2023-10-07
  • About author:YAN Shan-chun, E-mail: yanshanchun@126.com; #Correspondence JIANG Dun, Mobile: +86-18846831475, E-mail: 821880041@qq.com
  • Supported by:

    This research was supported by the project funded by the Natural Science Foundation of Heilongjiang Province, China (YQ2022C006) and the National Natural Science Foundation of China (32101526).

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摘要：

重金属污染已经被认定为一种影响害虫生物防治效率的环境变量，但当前对重金属胁迫下寄生蜂的寄生适应度了解较少。在本研究中，Cd暴露穿过美国白蛾蛹对周氏啮小蜂的寄生适合度被调查，以及围绕寄主昆虫的先天免疫和子代蜂的氧化状态调查了Cd暴露影响美国白蛾-周氏啮小蜂互作的作用机制。结果发现，Cd可从美国白蛾蛹转移到周氏啮小蜂子代蜂，且二者的转移系数反映了一种生物放大效应。未处理组和Cd处理组之间的寄生成功率和出蜂率无显著差异。然而，寄生Cd积聚的蛹后，子代蜂的寄生适应度（例如，数量、个体大小和寿命）显著降低。Cd胁迫下，美国白蛾蛹的细胞免疫和体液免疫水平显著下降。与未处理组相比，Cd处理组子代蜂的H2O2含量显著增加。Cd胁迫显著抑制了子代蜂超氧化物歧化酶的活性，但显著提高了抗坏血酸和谷胱甘肽的含量。这些结果表明。Cd暴露降低了周氏啮小蜂对美国白蛾蛹的循环防控效率。Cd暴露引起的子代蜂氧化状态紊乱可能是导致周氏啮小蜂对Cd积聚美国白蛾蛹寄生适合度降低的主要原因之一。

Abstract:

Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control, but the parasitic fitness of parasitoids under heavy metal stress is poorly understood.  Herein, the effect of Cd exposure through the host pupa of Hyphantria cunea on the parasitic fitness of Chouioia cunea was investigated, and the mechanism by which Cd exposure affects the interaction between H. cunea and C. cunea from the perspective of innate immunity in host insect and the oxidative status in the parasitoid offspring was explored.  Our results indicated that Cd can be transferred from the H. cunea pupae to the parasitoid offspring, and the transfer coefficient reflected biological amplification.  There were no significant differences in the rates of parasitism success and offspring emergence between the untreated and Cd-treated groups.  However, after parasitizing Cd-accumulated pupae, the parasitic fitness of offspring wasps (e.g., the number, individual size and life span) decreased significantly.  Under Cd exposure, the cellular and humoral immunity of H. cunea pupae decreased significantly.  Compared with the untreated group, the H2O2 content of parasitoid offspring in the Cd-treated group was significantly increased.  Cd exposure significantly inhibited superoxide dismutase activity in parasitoid offspring, but the contents of ascorbic acid and glutathione were significantly increased by Cd stress.  Taken together, these results indicate that Cd exposure reduces the cyclic utilization efficiency of C. cunea on H. cunea pupae.  The oxidative status of parasitoid offspring triggered by Cd exposure could be responsible for the reduced parasitic fitness of C. cunea on Cd-accumulated H. cunea pupae.

Key words: heavy metal ,  Hyphantria cunea ,  parasitic fitness ,  Chouioia cunea ,  oxidative status ,  innate immunity