硝酸盐响应转录因子MdNLP7通过调节生长素反应调控愈伤组织的形成

doi:10.1016/j.jia.2023.08.007

摘要/Abstract

摘要：

植物细胞具有全能性，在合适的培养条件下，已分化的植物细胞可以通过脱分化和再分化过程产生新的植物组织和器官。在这一过程中，生长素促进细胞生长与分裂，诱导愈伤组织的形成；细胞分裂素促进细胞的分裂并诱导不定芽的形成。硝酸盐不仅是植物生长发育必需的营养元素，还作为信号分子激活一系列基因的表达，进而影响植物生长发育。植物体内的硝酸盐信号通路还能够调控影响生长素的生物合成和运输，调控植物侧根的生长发育。MdNLP7是硝酸盐响应的主要调节因子，参与了植物体内硝酸盐的吸收和转运。在本研究中，将MdNLP7转录因子在拟南芥中异位表达，发现MdNLP7蛋白可以调控根外植体的再生；进一步的研究结果表明，MdNLP7介导了中柱鞘细胞分裂的起始。在愈伤组织形成的过程中，MdNLP7可以上调生长素合成和转运相关基因的表达，并通过影响生长素的分布来实现对根外植体形成的调控过程，进而调控硝酸盐介导的根外植体再生。

Abstract:

Under appropriate culture conditions, plant cells can regenerate new organs or even whole plants. De novo organ regeneration is an excellent biological system, which usually requires additional growth regulators, including auxin and cytokinin. Nitrate is an essential nutrient element for plant vegetative and reproductive development. It has been reported that nitrate is involved in auxin biosynthesis and transport throughout the growth and development of plants. In this study, we demonstrated that the ectopic expression of the MdNLP7 transcription factor in Arabidopsis could regulate the regeneration of root explants. MdNLP7 mainly participated in the regulation of callus formation, starting with pericycle cell division, and mainly affected auxin distribution and accumulation in the regulation process. Moreover, MdNLP7 upregulated the expression of genes related to auxin biosynthesis and transport in the callus formation stage. The results demonstrated that MdNLP7 may play a role in the nitrate-modulated regeneration of root explants. Moreover, the results revealed that nitrate–auxin crosstalk is required for de novo callus initiation and clarified the mechanisms of organogenesis.

Key words: MdNLP7 , callus initiation , auxin , nitrate , pericycle , shoot regeneration

LI Tong, FENG Zi-quan, ZHANG Ting-ting, YOU Chun-xiang, ZHOU Chao, WANG Xiao-fei. 硝酸盐响应转录因子MdNLP7通过调节生长素反应调控愈伤组织的形成[J]. Journal of Integrative Agriculture, 2023, 22(10): 3022-3033.

LI Tong, FENG Zi-quan, ZHANG Ting-ting, YOU Chun-xiang, ZHOU Chao, WANG Xiao-Fei. The nitrate-responsive transcription factor MdNLP7 regulates callus formation by modulating auxin response[J]. Journal of Integrative Agriculture, 2023, 22(10): 3022-3033.

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