Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (3): 710-724.DOI: 10.1016/S2095-3119(20)63590-0

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  • 收稿日期:2020-10-08 接受日期:2020-12-01 出版日期:2022-03-01 发布日期:2020-12-01

Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm

ZHANG Zhi-jun1, ZHANG Jing1, TANG Zhong-wen1, WANG Yan-peng1, GAO Teng-teng1, LIU Xiao-min1, MA Feng-wang1, LI Chao1   

  1. State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
  • Received:2020-10-08 Accepted:2020-12-01 Online:2022-03-01 Published:2020-12-01
  • About author:ZHANG Zhi-jun, E-mail: 1459030014@qq.com; Correspondence LI Chao, Tel/Fax: +86-29-87082648, E-mail: cl8609@nwafu.edu.cn
  • Supported by:
    This work was supported by the National Key Research and Development Program of China (2019YFD1001403–2B), the China Agriculture Research System of MOF and MARA (CARS-27), and the National Natural Science Foundation of China (31972389).

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摘要：

多巴胺是一种儿茶酚胺和一种抗氧化剂，在应对逆境时起作用，它与植物激素相互作用以介导植物发育。目前，关于苹果中多巴胺功能的研究较少。本研究开发了一种用于分析苹果种质中的多巴胺测定方法，以阐明多巴胺在苹果树的组织分布、发育变化、昼夜变化和逆境响应。首先，对所提出的方法进行了验证，定量的线性范围在0.1-20 ng mL-1范围内稳定，仪器、日间精密度和样品重复性相对标准偏差分别为1.024%、5.607%和7.237%，加标回收率大于100%，表明该方法的可行性及其适用于快速分析苹果属种质中的多巴胺。接下来，测量了322个苹果组织中的多巴胺含量。结果表明，苹果的多巴胺水平较低，叶片中多巴胺的平均含量高于果皮和果肉。多巴胺在栽培品种和野生种质中向右偏。最后，分析了组织特异性、发育变化、昼夜变化和对逆境的响应。在栽培品种‘皮诺娃’（Malus domestica）中，多巴胺含量在叶芽中最高，在果肉中最低。叶片和果肉中多巴胺含量随着栽培品种‘凉香’（Malus domestica）的生长发育而降低。与对照相比，干旱或盐胁迫后苹果叶片的多巴胺含量更高。在本研究中，建立了一种基于HPLC-MS的苹果多巴胺检测方法，并证明是一种稳健的方法。本研究为未来阐明苹果树中多巴胺的组织分布、发育变化、昼夜变化和逆境响应提供了一个框架。


Abstract: Dopamine is a catecholamine and an anti-oxidant which functions in responses to stress and it interacts with plant hormones to mediate plant development.  At present, there are few studies on the functions of dopamine in apple.  This study developed a method for dopamine determination which was used to analyze dopamine in Malus germplasm, in order to clarify the tissue distribution, developmental changes, diurnal variations, and stress responses in apple trees.  First, the proposed method was verified.  The linear range of quantification was robust from 0.1 to 20 ng mL–1.  The instrumental, inter-day precision, and sample repeatability relative standard deviations were 1.024, 5.607, and 7.237%, respectively.  The spiked recovery was greater than 100%, indicating the feasibility of the method and its suitability for the rapid analysis of dopamine in Malus.  Next, the dopamine content was measured in 322 Malus tissues.  The results showed that the dopamine level in Malus was low and the average dopamine content in leaf was higher than in peel and flesh.  The dopamine had a skewed distribution that deviated to the right in cultivars and wild accessions.  Finally, the tissue specificity, developmental changes, diurnal changes, and responses to stress were analyzed.  In cultivar ‘Pinova’ (Malus domestica), the dopamine concentration was the highest in leaf buds and lowest in flesh.  The dopamine contents in leaf and flesh decreased with the growth and development of cultivar ‘Liangxiang’ (Malus domestica).  The dopamine content of apple leaves was higher after either drought or salinity stress as compared to the control.  In this study, a dopamine detection method for apple was established based on HPLC-MS and shown to be a robust approach.  This study provides a framework for future research on elucidating the tissue distribution, developmental changes, diurnal variation, and stress responses of dopamine in apple trees.

Key words: dopamine ,  Malus ,  HPLC-MS ,  cultivar ,  wild accession