Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey

doi:10.1016/S2095-3119(20)63335-4

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (8): 2170-2179.DOI: 10.1016/S2095-3119(20)63335-4

所属专题：植物病理合辑Plant Protection—Plant Pathology；植物病毒合辑Plant Virus

收稿日期:2020-03-29 出版日期:2021-08-01 发布日期:2021-07-20

Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey

Hakan FIDAN¹, Pelin SARIKAYA¹, Kubra YILDIZ², Bengi TOPKAYA², Gozde ERKIS³, Ozer CALIS¹

¹Plant Protection Department, Faculty of Agriculture, Akdeniz University, The Campus, Konyaalti 07070, Antalya, Turkey
² Plant Health Department, Bati Akdeniz Agricultural Research Institute (BATEM), Muratpasa 07050, Antalya, Turkey
³ Antalya Directorate of Agricultural Quarantine, Republic of Turkey Ministry of Agriculture and Forestry, Muratpasa 07050, Antalya, Turkey

Received:2020-03-29 Online:2021-08-01 Published:2021-07-20
Contact: Correspondence Ozer CALIS, Tel: +90-242-3106536, Fax: +90-242-3102479, E-mail: ozercalis@akdeniz.edu.tr
About author:Hakan FIDAN, E-mail: hakanfidan@akdeniz.edu.tr;

摘要/Abstract

Abstract:

Tomato brown rugose fruit virus (ToBRFV) causes severe fruit loss in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants. It is an emerging Tobamovirus that is spreading globally. The major challenge is to develop a reliable method for the detection of the virus, and to better characterize the symptoms it causes. The aims of this study, therefore, were to characterize the symptom development on tomato and pepper plants, and to establish a reliable detection method for the virus. Following infection of the tomato and pepper plants with ToBRFV, the leaves turned chlorotic, mosaic or mottled, while the fruit became rugose, necrotic and marbled, and showed discoloration with yellow or brown spots. Transmission electron microscopy (TEM) revealed single rod-like virus particles characteristic of the Tobamoviruses. Classical reverse transcription PCR (RT-PCR) and quantitative PCR (qPCR) with specific primers and probes confirmed that the virus is ToBRFV. We found that the resistance genes from tomato, Tm-22, and pepper, L1, L2, L3 and L4, did not confer resistance to ToBRFV. Here, we present a PCR-based method as a diagnostic test for detecting ToBRFV in infected seeds. This method will help to prevent further spread of the virus in commercial seeds.

Key words: tomato , pepper , Tobamovirus , ToBRFV , resistance

. [J]. Journal of Integrative Agriculture, 2021, 20(8): 2170-2179.

Hakan FIDAN, Pelin SARIKAYA, Kubra YILDIZ, Bengi TOPKAYA, Gozde ERKIS, Ozer CALIS. Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey[J]. Journal of Integrative Agriculture, 2021, 20(8): 2170-2179.

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Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey

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