Transcriptome analysis for understanding the mechanism of dark septate endophyte S16 in promoting the growth and nitrate uptake of sweet cherry

doi:10.1016/S2095-3119(20)63355-X

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (7): 1819-1831.DOI: 10.1016/S2095-3119(20)63355-X

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-03-05 出版日期:2021-07-01 发布日期:2021-06-02

Transcriptome analysis for understanding the mechanism of dark septate endophyte S16 in promoting the growth and nitrate uptake of sweet cherry

WU Fan-lin^1*, QU De-hui^3*, TIAN Wei¹, WANG Meng-yun¹, CHEN Fei-yan¹, LI Ke-ke¹, SUN Ya-dong¹, SU Ying-hua⁴, YANG Li-na⁵, SU Hong-yan¹, WANG Lei²

¹ Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, School of Agriculture, Ludong University, Yantai 264025, P.R.China
² College of Life Sciences, Ludong University, Yantai 264025, P.R.China
³ State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
⁴ State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, P.R.China
⁵ Department of Genetics and Cell Biology, Basic Medical College, Qingdao University, Qingdao 266071, P.R.China

Received:2020-03-05 Online:2021-07-01 Published:2021-06-02
Contact: Correspondence SU Hong-yan, E-mail: suhongyan66 @126.com; WANG Lei, E-mail: wanglei9909@163.com
About author:WU Fan-lin, Mobile: +86-15965152366, E-mail: wufanlin1990 @126.com;* These authors contributed equally to this study.
Supported by:
This study was supported by the National Natural Science Foundation of China (31570649), the Key Research and Development Program of Yantai City, Shandong Province, China (2019XDHZ089), the Natural Science Foundation of Shandong Province, China (ZR2019PC052 and 2017C03), and the Key Research and Development Program of Shandong Province, China (2019GSF107091, 2019GSF109114, 2018YYSP004 and 2018GNC110028).

摘要/Abstract

摘要：

甜樱桃是世界最受欢迎的水果之一。早先，我们发现了一种黑色暗格真菌S16，它能够促进樱桃砧木吉塞拉5号的生长。然而，关于它们之间作用的分子机制还不甚了解。在本次研究中，我们分析了与S16共生的樱桃根部的生理指标以及转录组本，初步阐释了S16促樱桃生长的分子机制。与S16共生后，樱桃幼苗的活力更强。而且，与对照相比，S16共生根部共鉴定到4249个差异表达基因。这些基因涉及到与植物代谢、激素相关的生长过程。而且，与氮调控相关的基因高度富集。生理指标测定表明，S16能够促进樱桃幼苗利用NO₃^-转运蛋白来吸收氮源。因此，此次RNA测序数据库可以更加深入了从分子机制层面解析黑色暗格真菌促樱桃的生长过程。

Abstract:

Sweet cherry is one of the most popular fresh fruits in the world. Previously, we isolated a soilborne dark septate endophyte (DSE) strain, S16, which promoted the growth of Gisela 5 sweet cherry rootstock. However, little is known about the molecular mechanism of the effect of S16 on the growth of sweet cherry. In this study, the physiological parameters and transcript profiles of sweet cherry roots were analyzed under S16 treatment compared with a control to elucidate the molecular mechanisms of the effect of this strain on sweet cherry growth. After inoculation with S16, sweet cherry seedlings exhibited more vigorous growth. Moreover, we identified 4 249 differentially expressed genes (DEGs) between S16-treated plants and the control. Many of the DEGs are involved in pathways related to plant growth, such as cellular metabolic and plant hormone pathways. Additionally, some genes involved in nitrate regulation were also enriched; and these genes may be involved in the regulation of nitrate uptake in plants. Physiological index detection demonstrated that S16 could improve the nitrate assimilation of sweet cherry via NO₃^– transporters. This RNA-seq dataset provides comprehensive insight into the transcriptomic landscape to reveal the molecular mechanisms whereby the DSE influences the growth of sweet cherry.

Key words: sweet cherry , dark septate endophyte , RNA-seq , growth , NO₃^– transporters

. [J]. Journal of Integrative Agriculture, 2021, 20(7): 1819-1831.

WU Fan-lin, QU De-hui, TIAN Wei, WANG Meng-yun, CHEN Fei-yan, LI Ke-ke, SUN Ya-dong, SU Ying-hua, YANG Li-na, SU Hong-yan, WANG Lei. Transcriptome analysis for understanding the mechanism of dark septate endophyte S16 in promoting the growth and nitrate uptake of sweet cherry[J]. Journal of Integrative Agriculture, 2021, 20(7): 1819-1831.

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Transcriptome analysis for understanding the mechanism of dark septate endophyte S16 in promoting the growth and nitrate uptake of sweet cherry

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