Indica rice restorer lines with large sink potential exhibit improved nutrient transportation to the panicle, which enhances both yield and nitrogen-use efficiency

doi:10.1016/S2095-3119(20)63309-3

摘要/Abstract

摘要：

杂交组合的产量和氮素利用率与恢复系密切相关，因此，对高产氮高效型恢复系的农艺性状进行评价是十分必要的。然而，目前尚不清楚哪些骨干恢复系是高产氮高效型，它们的共同农艺性状也尚未被鉴定，在本研究中，我们旨在解决这个问题。我们在3个施氮量下实施了2个大田试验，从15个籼型恢复系中筛选出了5个高产氮高效型恢复系，并研究了不同产量和不同氮素利用率类型恢复系的产量、氮素利用率和养分转运情况。结果表明，产量和地上部总吸氮量随施氮量的增加而增加，而氮素稻谷生产效率随施氮量的增加而降低。高产氮高效型恢复系的每穗粒数较多，单穗重较高；而每穗粒数和单穗重均与产量和氮素利用率呈显著正相关；因此，较大的库容量可能有利于产量和氮素利用率的提高。我们进一步研究了高产氮高效型恢复系与低产氮低效型恢复系在养分向穗部转运方面的差异，发现与低产氮低效型恢复系相比，高产氮高效型恢复系在成熟期其穗部有更高的吸氮量和干物质量占比，在抽穗后有较高的单茎根系伤流强度和穗颈伤流强度，且其穗颈节和叶片维管束发达，有利于养分从根到地上部、从茎和叶到穗部的运输。因此，高产氮高效型恢复系的大库容量优势体现在养分在穗部的积累和分配较多，以及养分在运输通道上的畅通。

Abstract:

The yield and nitrogen use efficiency (NUE) of hybrid rice combinations are closely related to restorer line. Therefore, it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high NUE (HYHN). However, it is unclear which restorer lines are HYHN, and neither have the common agronomic traits of the HYHN restorer lines been identified. Aiming to address this issue, we conducted two filed experiments using three nitrogen applications, which screened five HYHN restorer lines from 15 indica restorer lines. Yield, NUE and nutrient transportation of restorer lines with different yields and NUE types were examined. Yield and total nitrogen absorption in aboveground biomass (TNA) increased, whereas NUE for grain production decreased with increasing nitrogen application levels. The HYHN restorer lines had large spikelets and high weight per panicle that were significantly positively correlated with yield and NUE. Therefore, large sink potential may be beneficial for both yield and NUE. We further studied the differences in nutrient transportation to panicles between the HYHN and low yield and low NUE (LYLN) restorer lines and found that the former had a higher nitrogen absorption level and dry matter weight ratios of panicle in maturity. Moreover, the HYHN lines also had a higher root and neck-panicle node bleeding intensity per stem after heading and more developed vascular bundles of neck-panicle nodes and leaves than the LYLN lines, which could contribute to the transportation of nutrients from root to ground and from stem and leaf to spike. Therefore, the advantages of large sink potential of the HYHN restorer lines include large nutrient accumulation in and distribution to the panicles and smooth flow of nutrients along the transportation channels.

Key words: indica hybrid rice , restorer lines , grain yield , nitrogen-use efficiency , sink potential , nutrient transportation

. [J]. Journal of Integrative Agriculture, 2021, 20(6): 1438-1456.

TIAN Qing-lan, HE Lian-hua, LIAO Shuang, LI Wu, DENG Fei, ZHOU Wei, ZHONG Xiao-yuan, REN Wan-jun. Indica rice restorer lines with large sink potential exhibit improved nutrient transportation to the panicle, which enhances both yield and nitrogen-use efficiency[J]. Journal of Integrative Agriculture, 2021, 20(6): 1438-1456.

参考文献

Barton L, Colmer T D. 2006. Irrigation and fertiliser strategies for minimising nitrogen leaching from turfgrass. Agricultural Water Managemment, 80, 160–175.
Campbell W H. 2002. Molecular control of nitrate reductase and other enzymes involved in nitrate assimilation. In: Oyer C H, Noctor G, eds., Photosynthetic Nitrogen Assimilation and Associated Carbon and Respiratory Metabolism. FKluwer Academic, The Netherlands. pp. 35–48.
Chen C, Zhang J, Li W, Tang D, Luo G, Wang J, Mo L, Lü M, Zhou J, Liang G, Huang J, Wang Y, Yao Y, Dong G. 2017. Fundamental features of source–sink characters and their regulation in high nitrogen efficiency rice lines. Chinese Journal of Rice Science, 31, 185–194. (in Chinese)
Chen Y, Peng J, Wang J, Fu P, Hou Y, Zhang C, Fahad S, Peng S, Cui K, Nie L, Huang J. 2015. Crop management based on multi-split topdressing enhances grain yield and nitrogen use efficiency in irrigated rice in China. Field Crops Research, 184, 50–57.
Cong R, Zhang Z, Lu J, Li X, Ren T, Wang W. 2015. Evaluation of nitrogen requirement and efficiency of rice in the region of Yangtze River Valley based on large-scale field experiments. Journal of Integrative Agriculture, 14, 2090–2098.
Dai G, Cheng S, Hua Z, Zhang M, Jiang H, Feng Y, Shen X, Su Y, He N, Ma Z, Ma X, Hou S, Wang Y. 2015. Mapping quantitative trait loci for nitrogen uptake and utilization efficiency in rice (Oryza sativa L.) at different nitrogen fertilizer levels. Geneticis and Molecular Research, 14, 10404–10414.
Deng F, Wang L, Mei X, Li S, Pu S, Ren W. 2016. Polyaspartate urea and nitrogen management affect nonstructural carbohydrates and yield of rice. Crop Science, 6, 3272–3285.
Deng F, Wang L, Ren W, Liu D, Wang W. 2012. Effects of planting methods on nitrogen accumulation and distribution of mid-late indica hybrid rice combination IIyou 498 under different ecological conditions. Scientia Agricultura Sinica, 45, 4310–4325. (in Chinese)
Dong G, Ju J, Li J, Yu X, Tian H, Zhang B, Zhou J, Wang Y. 2009a. Difference of source–sink components in conventional indica rice cultivars with different panicle weight. Scientia Agricultura Sinica, 42, 4187–4196. (in Chinese)
Dong G, Li J, Yu X, Zhou J, Tian H, Zhang Y, Zhang C, Zhang Y, Wang Y. 2009b. Characteristics of dry matter accumulation and distribution in conventional indica rice cultivars with different sink potentials. Chinese Journal of Rice Science, 23, 639–644. (in Chinese)
Feng Y, Chen H, Hu X, Zhou W, Xu F, Cai H. 2014. Nitrogen efficiency screening of rice cultivars popularized in South China. Journal of Plant Nutrition and Fertilizer, 20, 1051–1062. (in Chinese)
Godfray H C, Beddington J R, Crute I R, Haddad L, Lawrence D, Muir J, Pretty J, Robinson S, Thomas S, Toulmin C. 2010. Food security: The challenge of feeding 9 billion people. Science, 327, 812–818.
Guo J, Liu X, Zhang Y, Shen J, Han W, Zhang W, Christie P, Goulding K W T, Vitousek P M, Zhang F S. 2010. Significant acidification in major Chinese croplands. Science, 327, 1008–1010.
Hakeem K R, Ahmad A, Iqbal M, Gucel S, Ozturk M. 2011. Nitrogen-efficient rice cultivars can reduce nitrate pollution. Environmental Science and Pollution Research, 18, 1184–1193.
Hossain M, White S, Elahi S, Sultana N, Choudhury M, Alam Q, Rother J, Gaunt J. 2005. The efficiency of nitrogen fertiliser for rice in Bangladeshi farmers’ fields. Field Crops Research, 93, 94–107.
Huang Y, Li M, Lu M, Wan J, Long Q, Wang H, Tang X, Fan Z. 2015. Selection of rice germplasm with high nitrogen utilization efficiency and its analysis of the related characters. Journal of Plant Genetic Resources, 16, 87–93. (in Chinese)
Kant S, Bi Y, Rothstein S J. 2010. Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. Journal of Experimental Botany, 62, 1499–1509.
Ji L, Li T, Zhang X, Yu H. 2012. Root morphological and activity characteristics of rice genotype with high nitrogen utilization efficiency. Scientia Agricultura Sinica, 45, 4770–4781. (in Chinese)
Ladha J K, Pathak H, Krupnik T J, Six J, Kessel C. 2005. Efficiency of fertilizer nitrogen in cereal production: Retrospects and prospects. Advances in Agronomy, 87, 85–156.
Li M, Luo D, Jiang X, Zhou W, Gi G, Wang X, Li S. 2015. Dry matter production characteristics of rice cultivars with high yield and high N use efficiency. China Rice, 21, 65–67. (in Chinese)
Li M, Zhang H, Yang X, Ge M, Ma Q, Wei H, Dai Q, Huo Z, Xu K, Cao L, Wu H. 2012. Root morphological and physiological characteristics of rice cultivars with high yield and high nitrogen use efficiency. Acta Agronomica Sinica, 38, 648–656. (in Chinese)
Li P, Lu J, Hou W, Pan Y, Wang Y, Khan M R, Ren T, Cong R, Li X. 2017. Reducing nitrogen losses through ammonia volatilization and surface runoff to improve apparent nitrogen recovery of double cropping of late rice using controlled release urea. Environmental Science and Pollution Research, 24, 11722–11733.
Lin Z, Zhen C, Wu S, Wang Y, Tang Y. 1986. Studies on nitrate reductase activity and nitrogen repose in crop plants II. the uptake and assimilation of nitrate in indica and japonica rice. Acta Agronomica Sinica, 12, 9–14. (in Chinese)
Liu B, Tian Q, Zhong X, Zhao M, Huang G, Ma R, Ren W. 2015. Effects of mechanized planting methods on root traits of indica hybrid rice. Chinese Journal of Rice Science, 29, 490–500. (in Chinese)
Liu J, You L, Amini M, Obersteiner M, Herrero M, Zehnder A J B, Yang H. 2010. A high-resolution assessment on global nitrogen flows in cropland. Proceedings of the National Academy of Sciences of the United States of America, 107, 8035–8040.
Liu L, Chen T, Wang Z, Zhang H, Yang J, Zhang J. 2013. Combination of site-specific nitrogen management and alternate wetting and drying irrigation increases grain yield and nitrogen and water use efficiency in super rice. Field Crops Research, 154, 226–235.
Ma J, Zhu Q, Ma W, Tian Y, Yang J, Zhou K. 2003. Studies on the photosynthetic characteristics and accumulation and transformation of assimilation product in heavy panicle type of rice. Scientia Agricultura Sinica, 36, 375–381. (in Chinese)
Marsehner H, Kirby E A, Engels C. 1997. Importance of cycling and recycling of mineral nutrients within plants for growth and development. Plant Biology, 110, 265–273.
Ni J, An L. 1984. Kinetic analysis of NH4+ and K+ uptake in seedlings of hybrid rice. Acta Phytophysiologica Sinica, 4, 381–390. (in Chinese)
Pan S, Huang S, Zhai J, Cai M, Cao C, Zhang M, Tang X. 2012. Effects of nitrogen rate and its basal to dressing ratio on uptake, translocation of nitrogen and yield in rice. Soils, 44, 23–29.
Peng S, Buresh R J, Huang J, Yang J, Zhou Y, Zhong X, Wang G, Zhang F. 2006. Strategies for overcoming low agronomic nitrogen use efficiency in irrigated rice systems in China. Field Crops Research, 96, 37–47.
Peng S, Garcia F V, Laza R C, Sanico A L, Visperas R M, Cassman K G. 1996. Increased N-use efficiency using a chlorophyll meter on high-yielding irrigated rice. Field Crops Research, 47, 243–252.
Piao Z, Han L, Gao Z, Zhang J, Lu J, Li P. 2005. Analysis on combining ability of dry weight and nitrogen use efficiency in rice. Chinese Journal of Rice Science, 19, 527–532. (in Chinese)
Piao Z, Han L, Kon H, Lu J, Zang J. 2004. Selection effect of nitrogen use efficiency in rice. Acta Agronomica Sinica, 30, 651–656. (in Chinese)
Qin J, Yang Z, Sun Y, Xu H, Ma J. 2014. Differential comparison of assimilation products accumulation, nitrogen uptake and utilization and grain yield of hybrid rice combinations with different panicle types. Chinese Journal of Rice Science, 28, 514–522. (in Chinese)
Ray D, Mueller N, West P, Foley J. 2013. Yield trends are insufficient to double global crop production by 2050. PLoS ONE, 8, e66428.
Tian Q, Liu B, Zhong X, Zhao M, Sun H, Ren W. 2016. Relationship of NSC with the formation of branches and spikelets and the yield traits of indica hybrid rice in different planting methods. Scientia Agricultura Sinica, 49, 35–53. (in Chinese)
Vinod K K, Heuer S. 2002. Approaches towards nitrogen and phosphorus efficient rice. AoB Plants, 2012, pls028.
Wei D, Cui K, Ye G, Pan J, Xiang J, Huang J, Nie L. 2012. QTL mapping for nitrogen-use efficiency and nitrogen-deficiency tolerance traits in rice. Plant and Soil, 359, 281–295.
Wei H, Zhang H, Hang J, Dai Q, Huo Z, Xu K, Zhang S, Ma Q, Zhang Q, Zhang J. 2008. Characteristics of N accumulation and translocation in rice genotypes with different N use efficiencies. Acta Agronomica Sinica, 34, 119–125. (in Chinese)
Wu L, Yuan S, Huang L, Sun F, Zhu G, Li G, Fahad S, Peng S, Wang F. 2016. Physiological mechanisms underlying the high-grain yield and high-nitrogen use efficiency of elite rice varieties under a low rate of nitrogen application in China. Frontiers in Plant Science, 7, 1024.
Wu P, Tao Q. 1995. Genotypic response and selection pressure on nitrogen-use efficiency in rice under different nitrogen regimes. Journal of Plant Nutrition, 18, 487–500.
Xia L, Lam S, Shu K, Chen D, Yan X. 2017. Can knowledge-based N management produce more staple grain with lower greenhouse gas emission and reactive nitrogen pollution? A meta-analysis. Global Change Biology, 23, 1917–1925.
Xu F, Xiong H, Xie R, Zhang L, Zhu Y, Guo X, Yang D, Zhou X, Liu M. 2009. Advance of rice fertilezer-nitrogen use efficiency. Plant Nutrition and Fertilizer Science, 15, 1215–1225.
Yan C, Hong X, Ruan G,Yu S,Wang S, Ding Y. 2014. Studies on the accumulation and transformation of assimilation product of heavy panicle type rice using 13C labeling technique. Journal of Nuclear Agricultural Sciences, 28, 1282–1287. (in Chinese)
Yao F, Huang J, Cui K, Nie L, Xiang J, Liu X, Wu W, Chen M, Peng S. 2012. Agronomic performance of high-yielding rice variety grown under alternate wetting and drying irrigation. Field Crops Research, 126, 16–22.
Ye L, Lü H, Song W, Tu E, Shen Q. 2011. Variation of activity of N metabolizing enzymes in rice plants different in N efficiency at their late growth stages. Acta Pedologica Sinica, 48, 132–140. (in Chinese)
Ye Q, Zhang H, Wei H, Zhang Y, Wang B, Xia K, Huo Z, Dai Q, Xu K. 2005. Effects of nitrogen fertilizer on nitrogen use efficiency and yield of rice under different soil conditions. Scientia Agricultura Sinica, 31, 1422–1428. (in Chinese)
Yu Y, Huang Y, Zhang W. 2012. Changes in rice yields in China since 1980 associated with cultivar improvement, climate and crop management. Field Crops Research, 136, 65–75.
Yu Y, Wu W, Chen G, Zhou Y, Xu Y, Xi M, Yang C. 2015. Preliminary screening of high nitrogen utilization efficiency rice. China Rice, 21, 99–102. (in Chinese)
Zeng X, Han B, Xu F, Huang J, Cai H, Shi L. 2012. Effect of optimized fertilization on grain yield of rice and nitrogen use efficiency in paddy fields with different basic soil fertilities. Scientia Agricultura Sinica, 45, 2886–2894. (in Chinese)
Zhang H, Wang X, Dai Q, Huo Z, Xu K. 2003. Effects of N-application rate on yield, quality and characters of nitrogen uptake of hybrid rice variety Liangyoupeijiu. Scientia Agricultura Sinica, 36, 800–806. (in Chinese)
Zhang J, Liu J, Zhang J, Zhao F, Cheng Y, Wang W. 2010. Effects of nitrogen application rates on translocation of dry matter and utilization of nitrogen in rice and wheat. Acta Agronomica Sinica, 36, 1736–1742. (in Chinese)
Zhang J, Yang G, Li Y, Fan Y, Jiang F, Chen J, Hu Y. 2017. Effect of nitrogen level on combining ability of yield traits of hybrid rice. Journal of Northwest A&F University (Natural Science Edition), 45, 37–44.
Zhang S, He Q, Li Y, Lü T, Jiang M, Sun Y, Ma J. 2017. Effects of different water-nitrogen management patterns on grain yield and use efficiency of water and nitrogen of indica hybrid rice Fyou 498. Hybrid Rice, 32, 72–81. (in Chinese)
Zhang Y. 2006. Genotypic differences in grain yields and nitrogen use efficiency in rice. MSc thesis, Nanjing Agricultural University, China. (in Chinese)
Zhang Y, Tang Q, Zou Y, Li D, Qin J, Yang S, Chen L, Xia B, Peng S. 2009. Yield potential and radiation use efficiency of “super” hybrid rice grown under subtropical conditions. Field Crops Research, 114, 91–98.
Zhang Y, Wu R, Jiang N, Liu G. 1989. Relationship between nitrogen use efficiency and variety type of rice. Plant Physiology Communications, 2, 45–47.
Zhao Q, Gao E, Huang P, Ling Q. 1999. Relation between bleeding potential in neck of spike and source-sink quality of rice. Scientia Agricultura Sinica, 2, 104–106. (in Chinese)
Zhong L, He H, Pan X. 2011. Characteristics of the differentiation and retrogression of branchand spikelet in indica hybrid rice restoring lines and their F1. Acta Agriculturae Universitatis Jiangxiensis, 33, 0211–0216. (in Chinese)
Zhou K, Wang X, Li S, Li P, Li H, Huang G, Liu T, Shen M. 1997. The study on heavy panicle type of inter-subspecific hybrid rice (Oryza sativa L.). Scientia Agricultura Sinica, 30, 91–93. (in Chinese)
Zhou Y, Huang S, Li Q, Wei Y, Zeng S, Yang J, Zheng Y. 2015. Combining ability analysis on grain yield and its components of several backbone restorer lines in three-line hybrid rice. Hybrid Rice, 30, 68–73. (in Chinese)
Zhu D, Zhang Y, Chen H, Xiang J, Zhang Y. 2015. Innovation and practice of high-yield rice cultivation technology in China. Scientia Agricultura Sinica, 48, 3404–3414. (in Chinese)