Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants

doi:10.1016/S2095-3119(20)63415-3

摘要/Abstract

摘要：

植物促生真菌具有产生生物活性物质、促进植物生长以及增强植物免疫抗性的能力，其作为有应用价值的有益微生物在作物栽培中受到越来越多关注。本研究从健康番茄植株根际土壤中分离筛选到一株木霉菌株TM2-4，将其鉴定为非洲哈茨木霉（Trichoderma afroharzianum）。菌株TM2-4发酵滤液中含有多种生物活性物质且对番茄种子发芽具有明显促进作用，发酵滤液100倍稀释液处理，番茄胚轴、胚根长度，种子活力指数分别增加28.7%、19.4% 和 62.1%。为了评价菌株TM2-4的促生作用及其相关机制，通过盆栽试验和转录组测序分析了非洲哈茨木霉菌制剂TM2-4处理对番茄植株生物学指标和基因表达型的影响。结果表明，非洲哈茨木霉TM2-4能够通过在植株根际土壤和根系有效定殖，显著提高番茄株高、干重、单株叶片数及根系活力等生物学指标。转录组分析发现，木霉菌制剂处理番茄根系较对照共获得984个差异表达基因，主要集中在激素平衡、抗氧化活性以及苯丙烷类生物合成和谷胱甘肽代谢等生物学过程。相关研究结果为阐明菌株TM2-4对番茄的促生作用机制提供有效信息，并为非洲哈茨木霉微生物菌剂在蔬菜作物生产中的进一步开发应用奠定理论基础。

Abstract:

Plant growth promoting fungi are receiving increased attention as valuable beneficial microorganisms in crop cultivation due to their capacity to produce bioactive substances, promote plant growth and enhance immune defense functions. In this study, a novel Trichoderma isolate, designated as TM2-4, was screened from healthy tomato rhizosphere soil and identified as Trichoderma afroharzianum. Culture filtrate of the isolate TM2-4 displayed obvious bioactive substance production and an evident effect in promoting tomato seed germination, with hypocotyl length, radical length and vigor index increased by 28.7, 19.4 and 62.1%, respectively, after a 100-fold dilution treatment. To assess the promotion effect and related mechanism of isolate TM2-4, the plant biological indexes and gene expression profiles of tomato plants treated with or without T. afroharzianum TM2-4 microbial agent were investigated by greenhouse pot experiment and RNA sequencing. The results demonstrated that T. afroharzianum TM2-4 significantly promoted tomato plant growth in terms of plant height, dry weight, number of leaves per plant and root activity, through efficient colonization in the rhizosphere and root system of the plants. Transcriptome analyses identified a total of 984 differentially expressed genes in T. afroharzianum microbial agent inoculated tomato roots, which were mainly engaged in the biological process of phytohormone homeostasis, antioxidant activity, as well as metabolic pathways including phenylpropanoid biosynthesis and glutathione metabolism. These findings provide useful information for understanding the mechanism of isolate TM2-4 for tomato plant growth promotion, which would facilitate further development of T. afroharzianum TM2-4 microbial agent for use in vegetable crop production.

Key words: Trichoderma afroharzianum , Solanum lycopersicum L. , plant growth promotion , transcriptome analyses , qRT-PCR

. [J]. Journal of Integrative Agriculture, 2021, 20(5): 1266-1276.

ZHAO Juan, LIU Ting, LIU Wei-cheng, ZHANG Dian-peng, DONG Dan, WU Hui-ling, ZHANG Tao-tao, LIU De-wen. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants[J]. Journal of Integrative Agriculture, 2021, 20(5): 1266-1276.

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