Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions

doi:10.1016/S2095-3119(19)62825-X

摘要/Abstract

Abstract: Synthetic hexaploid wheat (SHW), possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties. We evaluated 10 root traits at seedling stage in 111 F₉ recombinant inbred lines derived from a F₂ population of a SHW line (SHW-L1) and a common wheat line, under normal (NC) and polyethylene glycol-simulated drought stress conditions (DC). We mapped quantitative trait loci (QTLs) for root traits using an enriched high-density genetic map containing 120 370 single nucleotide polymorphisms (SNPs), 733 diversity arrays technology markers (DArT) and 119 simple sequence repeats (SSRs). With four replicates per treatment, we identified 19 QTLs for root traits under NC and DC, and 12 of them could be consistently detected with three or four replicates. Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7% of the phenotypic variation respectively, and six novel QTLs for root fresh weight, the ratio of root water loss, total root surface area, number of root tips, and number of root forks under DC explained 8.5–14% of the phenotypic variation. Here seven of eight novel QTLs could be consistently detected with more than three replicates. Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.

Key words: synthetic hexaploid wheat , quantitative trait loci , drought stress , root traits

. [J]. Journal of Integrative Agriculture, 2020, 19(8): 1947-1960.

LIU Rui-xuan, WU Fang-kun, YI Xin, LIN Yu, WANG Zhi-qiang, LIU Shi-hang, DENG Mei, MA Jian, WEI Yu-ming, ZHENG You-liang, LIU Ya-xi. Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions[J]. Journal of Integrative Agriculture, 2020, 19(8): 1947-1960.

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