Investigating structural impact of a valine to isoleucine substitution on anti-Müllerian hormone in silico and genetic association of the variant and AMH expression with egg production in chickens

doi:10.1016/S2095-3119(20)63176-8

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (6): 1635-1643.DOI: 10.1016/S2095-3119(20)63176-8

所属专题：动物科学合辑Animal Science

收稿日期:2019-05-30 出版日期:2020-06-01 发布日期:2020-04-26

Investigating structural impact of a valine to isoleucine substitution on anti-Müllerian hormone in silico and genetic association of the variant and AMH expression with egg production in chickens

DANG Li-ping¹, LIU Rui-fang¹, ZHAO Wen-yan¹, ZHOU Wen-xin¹, MIN Yu-na¹, WANG Zhe-peng^{1, 2}

¹ College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R.China
² Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling 712100, P.R.China

Received:2019-05-30 Online:2020-06-01 Published:2020-04-26
Contact: Correspondence WANG Zhe-peng, Tel: +86-29-87092102, E-mail: wangzhepeng-001@163.com
Supported by:
This work was supported by the Natural Science Basic Research Plan in Shaanxi Province of China (2018JM3002), the Fundamental Research Funds for the Central Universities (2452019202) and the China Scholarship Council Scholarship (201906305010).

摘要/Abstract

Abstract:

Anti-Müllerian hormone (AMH) acts in maintaining orderly cyclic recruitment of early follicles, suggesting that it is a promising candidate for influencing animal reproductive efficiency. This study aimed to elucidate the effect of a missense mutation of Val566Ile on the structure of AMH protein and the genetic association of Val566Ile and AMH expression with egg production in chickens. Structural perturbations of Val566Ile were predicted by homology modeling. The association of the variant with the number of eggs was tested using a quantitative trait transmission disequilibrium test model. AMH expression in granulosa cells in Lueyang black-boned chickens was compared with that in Nick chickens. The Val⁵⁶⁶ of AMH is a non-conservative amino acid among mammals and birds, but its hydrophobicity is completely conservative. The substitution of Val⁵⁶⁶ for Ile⁵⁶⁶ potentially disrupted hydrogen bonds and solvent accessibility of 22 residues and created a short α-helix in the C terminus of AMH. Despite having striking structure-disrupting potential, the variant was not statistically associated with the number of eggs (P>0.05) in the Lueyang black-boned chickens. We did not detect differential expression of AMH between Lueyang black-boned chickens and Nick chickens (P>0.05). These results confirmed the structural impact of Val566Ile, but suggested that Val566Ile and AMH expression might not be the major genetic determinants for egg production in Lueyang black-boned chickens.

Key words: chicken , egg production , anti-Müllerian hormone , follicle recruitment

. [J]. Journal of Integrative Agriculture, 2020, 19(6): 1635-1643.

DANG Li-ping, LIU Rui-fang, ZHAO Wen-yan, ZHOU Wen-xin, MIN Yu-na, WANG Zhe-peng. Investigating structural impact of a valine to isoleucine substitution on anti-Müllerian hormone in silico and genetic association of the variant and AMH expression with egg production in chickens[J]. Journal of Integrative Agriculture, 2020, 19(6): 1635-1643.

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Investigating structural impact of a valine to isoleucine substitution on anti-Müllerian hormone in silico and genetic association of the variant and AMH expression with egg production in chickens

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