Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura

doi:10.1016/S2095-3119(20)63167-7

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (6): 1492-1500.DOI: 10.1016/S2095-3119(20)63167-7

收稿日期:2019-10-15 出版日期:2020-06-01 发布日期:2020-04-26

Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura

WEN Liang*, GAO Gui-ping*, HUANG Zhi-qiang, ZHENG Si-chun, FENG Qi-li, LIU Lin

Institute of Insect Science and Technology, School of Life Sciences, South China Normal University/Guangzhou Key Laboratory of Insect Development Regulation and Applied Research/Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou 510631, P.R.China

Received:2019-10-15 Online:2020-06-01 Published:2020-04-26
Contact: Correspondence LIU Lin, E-mail: liul@scnu.edu.cn
About author: * These authors contributed equally to this study.
Supported by:
The study was supported by the National Natural Science Foundation of China (31330071 and 31772519) and the Natural Science Foundation of Guangdong Province, China (2017A030313210).

摘要/Abstract

Abstract:

Fatty acid-binding proteins (FABPs) are a family of lipid chaperones, which contribute to systemic metabolic regulation through diverse lipid signalings. In this study, a midgut-specific FABP gene (Slfabp2) was cloned from Spodoptera litura. RT-PCR and Western blot analysis indicated that RNA and protein levels of SlFABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage. The expression of SlFABP2 protein was induced by starvation treatment. In vitro binding assay revealed that the recombinant SlFABP2 had high affinities of binding long-chain fatty acids, such as palmitic acid, arachidonate and oleic acid. The results suggest that SlFABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis. This work provides experimental clues for understanding the potential function of SlFABP2 in fatty acid metabolism in S. litura.

Key words: Spodoptera litura , SlFABP2 , starvation treatment , binding assay

. [J]. Journal of Integrative Agriculture, 2020, 19(6): 1492-1500.

WEN Liang, GAO Gui-ping, HUANG Zhi-qiang, ZHENG Si-chun, FENG Qi-li, LIU Lin. Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura[J]. Journal of Integrative Agriculture, 2020, 19(6): 1492-1500.

参考文献

Caccia S, Grimaldi A, Casartelli M, Falabella P, Eguileor M D, Pennacchio F, Giordana B. 2012. Functional analysis of a fatty acid binding protein produced by Aphidius ervi teratocytes. Insect Physiology, 58, 621–627.
Cheng L, Jin X K, Li W W, Li S, Guo X N, Wang J, Gong Y N, He L, Wang Q. 2013. Fatty acid binding proteins FABP9 and FABP10 participate in antibacterial responses in Chinese mitten crab, Eriocheir sinensis. PLoS ONE, 8, e54053.
Chmurzynaka A. 2006. The multigene family of acid-binding proteins (FABPs): Function, structure and polymorphism. Journal of Applied Genetics, 47, 39–48.
Currie E, Schulze A, Zechner R, Walther T C, Farese R V. 2013. Cellular fatty acid metabolism and cancer. Cell Metabolism, 18, 153–161.
Esteves A, Ehrlich R. 2006. Invertebrate intracellular fatty acid-binding proteins. Comparative Biochemistry and Physiology (Part C: Toxicology & Pharmacology), 142, 262–274.
Falabella P, Perugino G, Caccialupi P, Riviello L, Varricchio P, Tranfaglia A, Rossi M, Malva C, Graziani F, Moracci M, Pennacchio F. 2005. A novel fatty acid binding protein produced by teratocytes of the aphid parasitoid Aphidius ervi. Insect Molecular Biology, 14, 195–205.
Furuhashi M. 2017. Fatty acid-binding proteins, a family of lipid chaperones. In: Geiger O, ed., Handbook of Hydrocarbon and Lipid Microbiology. Springer International Publishing, Switzerland. pp. 1–16.
Furuhashi M, Hotamisligil G S. 2008. Fatty acid-binding proteins: Role in metabolic diseases and potential as drug targets. Nature Reviews Drug Discovery, 7, 489–503.
Gao C, Bei Y, Chen T, Gu X. 2004. On factors causing the outbreak of Spodoptera litura (Fabricius). Acta Agriculturae Zhejiangensis, 16, 332–335. (in Chinese)
GB/T 17376-2008. 1998. Animal and Vegetable Fats and Oils - Preparation of Methyl Esters of Fatty Acids. National Standard of the People’s Republic of China. (in Chinese)
Gerstner J R, Vanderheyden W M, Shaw P J, Landry C F, Yin J C. 2011. Cytoplasmic to nuclear localization of fatty-acid binding protein correlates with specific forms of long-term memory in Drosophila. Communicative & Integrative Biology, 4, 623–626.
Hagopian K, Munday M R. 1997. The role of pyruvate dehydrogenase, phosphofructo-1-kinase and acetyl-CoA carboxylase in the regulation of fatty acid synthesis in the lactating rat mammary gland during the starved to re-fed transition. Biochimica et Biophysica Acta General Subjects, 1336, 474–484.
Haunerland N H, Andolfatto P, Chisholm J M, Wang Z, Chen X. 1992. Fatty-acid-binding protein in locust flight muscle: Developmental changes of expression, concentration and intracellular distribution. The FEBS Journal, 210, 1045–1051.
Haunerland N H, Chen X H, Andolfatto P, Chisholm J M, Wang Z. 1993. Developmental changes of FABP concentration, expression and intracellular distribution in locust flight muscle. Molecular & Cellular Biochemistry, 123, 153–158.
Huang Z Q, Zhou D H, Gao G P, Zheng S C, Feng Q L, Liu L. 2012. Cloning and characterization of midgut-specific fatty acid binding protein in Spodoptera litura. Archives of Insect Biochemistry & Physiology, 79, 1–17.
JY/T 003-1996. 1997. General Principles for Organic Mass Spectrometry. Educational Standard of the People’s Republic of China. (in Chinese)
Kane C D, Bernlohr D A. 1996. A simple assay for intracellular lipid-binding proteins using displacement of 1-anilinonaphthalene-8-sulfonic acid. Analytical Biochemistry, 233, 197–204.
Kaur M, Saraf I, Kumar R, Singh I P, Kaur S. 2019. Bioefficacy of hexane extract of Inula racemosa (Asteraceae) against Spodoptera litura (Lepidoptera: Noctuidae). Gesunde Pflanzen, 71, 165–174.
King-Siang G, Chia-Wei L, Dafydd J. 2011. A Photocytes-associated fatty acid-binding protein from the light organ of adult Taiwanese firefly, Luciola cerata. PLoS ONE, 6, e29576.
Liu Y, Zhou S, Ma L, Tian L, Wang S, Sheng Z, Jiang R J, Willian G, Bendena Li S. 2010. Transcriptional regulation of the insulin signaling pathway genes by starvation and 20-hydroxyecdysone in the Bombyx fat body. Journal of Insect Physiology, 56, 1436–1444.
Lucke C, Qiao Y, Moerkerk H T, Veerkamp J H, Hamilton J A. 2006. Fatty-acid-binding protein from the flight muscle of Locusta migratoria: Evolutionary variations in fatty acid binding. Biochemistry, 45, 6296–6305.
Sha R S, Kane C D, Xu Z H, Banaszak L J, Bernlohr D A. 1993. Modulation of ligand-binding affinity of the adipocyte lipid-binding protein by selective mutation - Analysis in vitro and in situ. Journal of Biological Chemistry, 268, 7885–7892.
Shogo S, Narumi K, Koichiro K, Akira A, Hiroshi F. 2018. Fatty acid-binding protein 5 (FABP5) promotes lipolysis of lipid droplets, de novo fatty acid (FA) synthesis and activation of nuclear factor-kappa B (NF-κB) signaling in cancer cells. Biochimica et Biophysica Acta (Molecular and Cell Biology of Lipids), 1863, 1057–1067.
Smathers R L, Petersen D R. 2011. The human fatty acid-binding protein family: Evolutionary divergences and functions. Human Genomics, 5, 170–191.
Smith A F, Kozo T, Hanneman E, Suzuki T C, Wells M A. 1992. Isolation, characterization, and cDNA sequence of two fatty acid-binding proteins from the midgut of Manduca sexta larvae. Journal of Biological Chemistry, 267, 380–384.
Storch J, Thumser A E. 2000. The fatty acid transport function of fatty acid-binding proteins. Biochimica et Biophysica Acta, 1486, 28–44.
Storch J, Thumser A E. 2010. Tissue-specific functions in the fatty acid-binding protein family. Journal of Biological Chemistry, 285, 32679–32683.
Tan S J, Zhang X, Jin X K, Li W W, Li J Y, Wang Q. 2015. Fatty acid binding protein FABP3 from Chinese mitten crab Eriocheir sinensis participates in antimicrobial responses. Fish & Shellfish Immunology, 43, 264–274.
Velkov T, Chuang S, Wielens J, Sakellaris H, Charman W N, Porter C J H, Scanlon M. 2005. The interaction of lipophilic drugs with intestinal fatty acid-binding protein. Journal of Biological Chemistry, 280, 17769–17776.
Zhang Y, King M O, Harmon E, Swanson D L. 2015. Summer-to-winter phenotypic flexibility of fatty acid transport and catabolism in skeletal muscle and heart of small birds. Physiological and Biochemical Zoology, 88, 535–549.
Zimmerman A W, Veerkamp J H. 2002. New insights into the structure and function of fatty acid-binding proteins. Cellular and Molecular Life Sciences, 59, 1096–1116.

Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura

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