Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)

doi:10.1016/S2095-3119(19)62777-2

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (2): 528-540.DOI: 10.1016/S2095-3119(19)62777-2

所属专题：园艺-栽培生理/资源品质合辑Horticulture — Physiology · Biochemistry · Cultivation

收稿日期:2019-02-11 出版日期:2020-02-01 发布日期:2020-01-18

Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)

ZHU Ying-chun^{1, 2}, SUN De-xi², DENG Yun², AN Guo-lin², LI Wei-hua², SI Wen-jing², LIU Jun-pu², SUN Xiao-wu¹

¹College of Horticulture, Hunan Agricultural University, Changsha 410128, P.R.China
² Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.China

Received:2019-02-11 Online:2020-02-01 Published:2020-01-18
Contact: Correspondence LIU Jun-pu, Tel/Fax: +86-371-60247661, E-mail: liujunpu@caas.cn; SUN Xiao-wu, E-mail: sunxiaowu2007@126.com
About author: ZHU Ying-chun, E-mail: zhuyingchun@caas.cn;
Supported by:
This research was supported by the earmarked fund for China Agriculture Research System (CARS-25), the Fundamental Research Funds of the Chinese Academy of Agricultural Sciences (Y2018YJ15 and Y2019XK16-03), the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2018-ZFRI), and the National Key R&D Program of China (2018YFD0201310).

摘要/Abstract

Abstract:

In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding. However, because of low repeatability, embryoid induction rate and quality, the molecular mechanisms remain poorly understood. Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway, which induces the occurrence of haploid. In this study, unfertilized ovaries were heat shocked for 0 h (A0) before flowering and for 0 h (A1), 4 h (A3), 8 h (A5), 12 h (A7), and 24 h (A8), respectively, at 37°C at the first day of the flowering stage. The ovule enlargement rate was increased from 0% at 25°C to 96.8% at 37°C (24 h treatment). Thus, we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology. The results showed that compared with A3, A5, A7, and A8, the biosynthesis of amino acid, glycine, serine and threonine metabolic pathways in A1 has changed significantly. This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion. The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences. The results provide new information on the complex relationship between in vitro gynogenesis and temperature. This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.

Key words: watermelon , heat shock , unfertilized ovule , ovule enlargement , transcription factors

. [J]. Journal of Integrative Agriculture, 2020, 19(2): 528-540.

ZHU Ying-chun, SUN De-xi, DENG Yun, AN Guo-lin, LI Wei-hua, SI Wen-jing, LIU Jun-pu, SUN Xiao-wu.

Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)

[J]. Journal of Integrative Agriculture, 2020, 19(2): 528-540.

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Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)

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